CSN5/JAB1 suppresses the WNT inhibitor DKK1 in colorectal cancer cells

Jumpertz, Sandra; Hennes, Thomas; Asare, Yaw; Schütz, Anke K.; Bernhagen, Jürgen

doi:10.1016/j.cellsig.2017.02.013

Cited by 34 publications

(31 citation statements)

References 52 publications

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“…For instance, DKK1 was overexpressed in pancreas carcinoma and non-small cell lung cancer where it promotes cancer cells to migrate, invade and proliferate [22, 23]. A downregulation of DKK1 has been observed in melanoma and colon cancer where it functioned as a tumor suppressor gene [24, 25]. In this study, our results showed DKK1 overexpression resulted in decreased cell migration and invasion in breast cancer cells.…”

Section: Discussionsupporting

confidence: 51%

DKK1 inhibits breast cancer cell migration and invasion through suppression of β-catenin/MMP7 signaling pathway

Niu

Wang

et al. 2019

Cancer Cell Int

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Background DKK1 has been reported to act as a tumor suppressor in breast cancer. However, the mechanism of DKK1 inhibits breast cancer migration and invasion was still unclear. Methods Western blot and real time PCR was used to detect the expression of DKK1, β-catenin and MMP7 in breast cancer cells. Wound scratch assay and transwell assay was employed to examine migration and invasion of breast cancer cell. Results DKK1 overexpression dramatically inhibits breast cancer cell migration and invasion. Knockdown of DKK1 promotes migration and invasion of breast cancer cells. DKK1 suppressed breast cancer cell migration and invasion through suppression of β-catenin and MMP7 expression. XAV-939, an inhibitor of β-catenin accumulation could reverse DKK1 silencing-induced MMP7 expression in breast cancer cells. Meanwhile, XAV-939 also could reverse the increase in the cell number invaded through Matrigel when DKK1 was knockdown. Furthermore, depletion of MMP7 also could reverse DKK1 knockdown-induced increase in the cell number invaded through Matrigel. Conclusions DKK1 inhibits migration and invasion of breast cancer cell through suppression of β-catenin/MMP7 pathway, our findings offered a potential alternative for breast cancer prevention and treatment.

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Section: Discussionsupporting

confidence: 51%

DKK1 inhibits breast cancer cell migration and invasion through suppression of β-catenin/MMP7 signaling pathway

Niu

Wang

et al. 2019

Cancer Cell Int

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“…The researchers found that SIK1 silencing may promote the metastasis of HCC, cell migration and lung metastasis, and that the loss of SIK1 may activate the Wnt/β-catenin signaling pathway, which mediates HCC cell proliferation and invasion. The Wnt/β-catenin signaling pathway may influence cellular differentiation and is associated with a variety of human cancers, including CRC 39,40. In the present study, we further investigated the prognostic value of SIK1 in CRC.…”

Section: Discussionmentioning

confidence: 93%

<p>MicroRNA-17 promotes cell proliferation and migration in human colorectal cancer by downregulating SIK1</p>

Huang¹,

Liu²,

Xu³

et al. 2019

CMAR

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Purpose: There is mounting evidence to indicate that microRNA-17 (miR-17) is expressed and functionally involved in human cancers. However, the molecular mechanism underlying the role of miR-17 in colorectal cancer (CRC) remains largely unclear. This study aims to reveal the biological function of miR-17 in colorectal cancer. Materials and methods: The expression of miR-17 in CRC cells and tissues was examined using qRT-PCR. Cell proliferation and migration assays were performed after transfection with an miR-17 mimic and inhibitors. The potential gene targets of miR-17 were predicted by bioinformatics analysis and further validated by PCR, Western blot and dual luciferase reporter assays. Results: The expression of miR-17 was significantly upregulated in CRC cell lines and tissues and may imply poor prognosis. miR-17 upregulation promoted cell invasion and migration in CRC cell lines in vitro, while downregulation of miR-17 inhibited tumor progression. SIK1 was identified as a potential direct target of miR-17 by dual luciferase reporter assay, and its downregulation in CRC may suggest poor prognosis. Conclusions: Our study indicated that upregulated miR-17 may promote the progression of CRC and may exert its function as a tumor suppressor miRNA by targeting SIK1 .

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“…On the contrary, Rouanne et al (13) showe that the expression level of serum OPN is related to the pathological features of NSCLC and has some value in the diagnosis of NSCLC. DKK1 is a secretory glycoprotein and has been demonstrated to be expressed abnormally in colorectal cancer, gastric cancer, endometrial carcinoma and serum samples (14)(15)(16). It has been used as a new tumor marker and therapeutic target for esophageal and hepatic carcinoma (17).…”

Section: Introductionmentioning

confidence: 99%

Comparison of the diagnostic value of CEA combined with OPN or DKK1 in non‑small cell lung cancer

2020

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Carcinoma embryonic antigen (CEA), osteopontin (OPN), and Dickkopf-1 (DKK1) expressed in serum are associated with hypoxia in tumor progression. However, the role of these proteins in the plasma of patients with non-small cell lung cancer (NSCLC) is poorly understood. The diagnostic values of CEA combined with OPN or DKK1 were compared in non-small cell lung cancer. This study investigated the diagnostic value of CEA combined with OPN and DKK1, respectively, in NSCLC. Eighty patients with NSCLC (NSCLC group) and 60 patients with benign lung diseases (benign lung disease group) admitted to Shandong Provincial Third Hospital from May 2014 to January 2015 were selected as the study subjects. In addition, 60 healthy subjects undergoing normal physical examination were selected as healthy control group. The OPN and DKK1 in serum of the two groups were detected by enzyme linked immunosorbent assay (ELISA), and the CEA expression was measured by Electrochemical Photometric method. The diagnostic value of CEA combined with OPN and DKK1, respectively, in NSCLC was analyzed. The expression of CEA, OPN, and DKK1 in serum of NSCLC group was significantly higher than that of healthy control group and benign lung disease group (P<0.05). The expression of CEA, OPN and DKK1 in serum of NSCLC patients was correlated with tumor diameter, lymph node metastasis, degree of pathological differentiation and clinical stage (P<0.05). ROC curve for diagnosis of NSCLC was drawn by further combination of serum CEA and OPN. The AUC of combined diagnosis of CEA and OPN for NSCLC was 0.920 (95% CI, 0.875-0.964), and the diagnostic sensitivity and specificity were 87.50 and 86.67%, respectively; the AUC of combined diagnosis of CEA and DKK1 for NSCLC was 0.912 (95% CI, 0.866-0.958), and the diagnostic sensitivity and specificity were 92.50 and 76.67%, respectively. CEA, OPN and DKK1 may be involved in the occurrence and progression of NSCLC and have good sensitivity and specificity in the diagnosis of NSCLC and may be new biomarkers for the diagnosis of NSCLC.

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CSN5/JAB1 suppresses the WNT inhibitor DKK1 in colorectal cancer cells

Cited by 34 publications

References 52 publications

DKK1 inhibits breast cancer cell migration and invasion through suppression of β-catenin/MMP7 signaling pathway

DKK1 inhibits breast cancer cell migration and invasion through suppression of β-catenin/MMP7 signaling pathway

<p>MicroRNA-17 promotes cell proliferation and migration in human colorectal cancer by downregulating SIK1</p>

Comparison of the diagnostic value of CEA combined with OPN or DKK1 in non‑small cell lung cancer

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