CsIVP Modulates Low Nitrogen and High-Temperature Resistance in Cucumber

Abstract

Crop plants experience various abiotic stresses that reduce yield and quality. Although several adaptative physiological and defense responses to single stress have been identified, the behavior and mechanisms of plant response to multiple stresses remain underexamined. Herein, we determined that the leaf and vascular changes in CsIVP-RNAi cucumber plants can enhance resistance to nitrogen deficiency and high-temperature stress. CsIVP negatively regulated high nitrate affinity transporters (NRT2.1, NRT2.5) and reallocation transporters (NRT1.7, NRT1.9, NRT1.12) under low nitrogen stress. Furthermore, CsIVP-RNAi plants have high survival rate with low heat injury level under high temperature condition. Several key high-temperature regulators, including Hsfs, Hsps, DREB2C, MBF1b, and WRKY33 have significant expression in CsIVP-RNAi plants. CsIVP, act as a repressor, mediated high temperature responsive by physically interacting with CsDREB2C. Altogether, these results indicated that CsIVP integrates innate programming of plant development, nutrient transport, and high-temperature resistance, providing a potentially valuable target for breeding nutrient-efficient and heat-resistant crops.

Transcription Cofactor CsMBF1c Enhances Heat Tolerance of Cucumber and Interacts with Heat-Related Proteins CsNFYA1 and CsDREB2

“…As a TF, MBF1c may generally bind to specific DNA sequences . Since CsMBF1c has no transcriptional activation (Figure ), this suggests that CsMBF1c may act as a key transcriptional cofactor by combining TFs to regulate the tolerance of cucumber to heat. , CsMBF1c can interact with CsDREB2 and CsNFYA1 in organisms (Figures and S5). DREB2 plays an important role in the regulatory network of the responses to high temperatures. , In addition, DREB2 has been reported to coactivate heat-related genes with NFYA2/B3/DPB3-1 trimers in Arabidopsis . , NF-YA primarily regulates various life processes by binding to other proteins to form complexes in plants. − In cucumber, CsNFYA1 is involved in the regulation of flowering .…”

“…32 Since CsMBF1c has no transcriptional activation (Figure 7), this suggests that CsMBF1c may act as a key transcriptional cofactor by combining TFs to regulate the tolerance of cucumber to heat. 10,35 CsMBF1c can interact with CsDREB2 and CsNFYA1 in organisms (Figures 7 and S5). DREB2 plays an important role in the regulatory network of the responses to high temperatures.…”

“…9 CsIVP affects the physiological basis of cucumber and enhances its tolerance to heat. 10 The overexpression of CsLEA11 enhanced the resistance of Escherichia coli to heat. 11 Currently, little research has been conducted on the heat resistance of cucumber.…”

Genetic regulation of nitrogen use efficiency in Gossypium spp

“…Transgenic lines overexpressing NRT1.7 in Arabidopsis , rice and tobacco plants exhibited increased NO 3 − accumulation in growing tissues by recycling N from older leaves (Chen et al, 2020). Yan et al (2022) showed that RNAi‐mediated silencing of IRREGULAR VASCULATURE PATTERNING ( IVP ) in Cucumis sativus promotes plant tolerance to N deficiency and high‐temperature stress by negatively regulating the expression of NO 3 − transporters (NRT2.1 and NRT2.5) and recyclers (NRT1.7, NRT1.9 and NRT 1.12) under low NO 3 − conditions. NRT2.4 and NRT2.5 are highly expressed in major and minor veins for the removal of apoplastic NO 3 − under low N supply for phloem transport (Kiba et al, 2012; Lezhneva et al, 2014).…”

A quick and effective method for thermostability differentiation in cucumber (Cucumis sativus L.)

“…It prefers warm temperatures, but when the temperature surpasses 35°C, the growth of cucumbers is impeded (Woycicki et al, 2011; Chen et al, 2021). When ambient temperatures reach 50°C, leaf and stem undergo rapid wilting, root growth is retarded, pollen viability is diminished, the fruit is deformed, and it might even result in plant death, all leading to compromised fruit quality and commodity yield (Yu et al, 2018; Liu et al, 2021; Yan et al, 2022; Yu et al, 2022).…”