CsfG, a sporulation-specific, small non-coding RNA highly conserved in endospore formers

Marchais, Antonin; Duperrier, Sandra; Durand, Sylvain; Gautheret, Daniel; Stragier, Patrick

doi:10.4161/rna.8.3.14998

Cited by 31 publications

(33 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The identification of the "ylbH leader" thus seems to be an artifact caused by the antisense location of CsfG/S547. In addition, Marchais et al found that the sporulation-specific expression of CsfG/S547 is due to regulation by SigG and SigF (117). Importantly, their transcriptional fusion data are in accordance with the unsupervised promoter cluster analysis by Nicolas et al (15).…”

Section: Independently Expressed Small Rnas and Rna Antitoxinssupporting

confidence: 66%

Regulatory RNAs in Bacillus subtilis: a Gram-Positive Perspective on Bacterial RNA-Mediated Regulation of Gene Expression

Mars

Nicolas

Denham

et al. 2016

Microbiol Mol Biol Rev

View full text Add to dashboard Cite

SUMMARY Bacteria can employ widely diverse RNA molecules to regulate their gene expression. Such molecules include trans -acting small regulatory RNAs, antisense RNAs, and a variety of transcriptional attenuation mechanisms in the 5′ untranslated region. Thus far, most regulatory RNA research has focused on Gram-negative bacteria, such as Escherichia coli and Salmonella . Hence, there is uncertainty about whether the resulting insights can be extrapolated directly to other bacteria, such as the Gram-positive soil bacterium Bacillus subtilis . A recent study identified 1,583 putative regulatory RNAs in B. subtilis , whose expression was assessed across 104 conditions. Here, we review the current understanding of RNA-based regulation in B. subtilis , and we categorize the newly identified putative regulatory RNAs on the basis of their conservation in other bacilli and the stability of their predicted secondary structures. Our present evaluation of the publicly available data indicates that RNA-mediated gene regulation in B. subtilis mostly involves elements at the 5′ ends of mRNA molecules. These can include 5′ secondary structure elements and metabolite-, tRNA-, or protein-binding sites. Importantly, sense-independent segments are identified as the most conserved and structured potential regulatory RNAs in B. subtilis . Altogether, the present survey provides many leads for the identification of new regulatory RNA functions in B. subtilis .

show abstract

Section: Independently Expressed Small Rnas and Rna Antitoxinssupporting

confidence: 66%

Regulatory RNAs in Bacillus subtilis: a Gram-Positive Perspective on Bacterial RNA-Mediated Regulation of Gene Expression

Mars

Nicolas

Denham

et al. 2016

Microbiol Mol Biol Rev

View full text Add to dashboard Cite

show abstract

“…They regulate their target genes with similar mechanism, via binding with their targets by base pairing to regulate the expression of target genes. They usually share same sequences with their targets for base pairing, which are the basis of sRNA targets prediction by bioinformatics [3,[25][26][27]. Up to now, only limited essential sRNAs have been found in bacteria.…”

Section: Discussionmentioning

confidence: 99%

Esre: A novel essential non‐coding RNA in Escherichia coli

Chen¹,

Wang²,

Li³

et al. 2012

FEBS Letters

View full text Add to dashboard Cite

Edited by Tamas DalmayKeywords: Escherichia coli sRNA Essential sRNA Esre yigP a b s t r a c t YigP gene (GeneID: 948915) locates between ubiquinone biosynthetic genes ubiE and ubiB in Escherichia coli. GeneBank annotates yigP as a putative protein-coding gene. In this study, we found a new essential sRNA gene, esre, locates within the region of yigP. The E. coli strain with inactive esre must rely on a complementary plasmid to survive. Moreover, RACE experiments showed esre encodes an RNA molecule of 252 nt. Further experiments revealed esre gene is immune to frame shift mutations and the function of esre depends mostly on the RNA secondary structure, which are typical traits of sRNA. Since it is difficult to predict the target of an essential sRNA, more research is needed to reveal the function and mechanism of esre.

show abstract

“…VirX is not found in Clostridium tetani and C. difficile but is present in other clostridial species. Several sporulation-specific sRNAs, including SurA, SurC, and CsfG, have also been recently identified in B. subtilis, some of which are conserved in other endospore formers (99)(100)(101). However, their function during the sporulation process remains to be characterized.…”

Section: Discussionmentioning

confidence: 99%

Pleiotropic Role of the RNA Chaperone Protein Hfq in the Human Pathogen Clostridium difficile

et al. 2014

View full text Add to dashboard Cite

cClostridium difficile is an emergent human pathogen and the most common cause of nosocomial diarrhea. Our recent data strongly suggest the importance of RNA-based mechanisms for the control of gene expression in C. difficile. In an effort to understand the function of the RNA chaperone protein Hfq, we constructed and characterized an Hfq-depleted strain in C. difficile. Hfq depletion led to a growth defect, morphological changes, an increased sensitivity to stresses, and a better ability to sporulate and to form biofilms. The transcriptome analysis revealed pleiotropic effects of Hfq depletion on gene expression in C. difficile, including genes encoding proteins involved in sporulation, stress response, metabolic pathways, cell wall-associated proteins, transporters, and transcriptional regulators and genes of unknown function. Remarkably, a great number of genes of the regulon dependent on sporulation-specific sigma factor, SigK, were upregulated in the Hfq-depleted strain. The altered accumulation of several sRNAs and interaction of Hfq with selected sRNAs suggest potential involvement of Hfq in these regulatory RNA functions. Altogether, these results suggest the pleiotropic role of Hfq protein in C. difficile physiology, including processes important for the C. difficile infection cycle, and expand our knowledge of Hfq-dependent regulation in Gram-positive bacteria.

show abstract

CsfG, a sporulation-specific, small non-coding RNA highly conserved in endospore formers

Cited by 31 publications

References 26 publications

Regulatory RNAs in Bacillus subtilis: a Gram-Positive Perspective on Bacterial RNA-Mediated Regulation of Gene Expression

Regulatory RNAs in Bacillus subtilis: a Gram-Positive Perspective on Bacterial RNA-Mediated Regulation of Gene Expression

Esre: A novel essential non‐coding RNA in Escherichia coli

Pleiotropic Role of the RNA Chaperone Protein Hfq in the Human Pathogen Clostridium difficile

Contact Info

Product

Resources

About