CSF Protein Contents and Their Roles in Brain Development

Nabiuni, Mohammad; Shokohi, Rozmehr; Moghaddam, Parisa

doi:10.17795/zjrms-1042

Cited by 4 publications

(9 citation statements)

References 45 publications

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“…In this paper hDPSCs cultured with the medium supplemented with E-CSF, we hypothesized that adding E-CSF to the culture medium could provide a suitable molecules for differentiation of hDPSCs into neuron similar to a microenvironment provided in embryonic period. Also, in this study, the results showed that our treated cells survive in sequential passages without losing their ability to proliferate which is probably related to HGF/c-Met signaling that caused to development and maintenance of the nervous system [17], also, the results of MTT assay showed that the neuron-like cells viability after induction by E-CSF was significantly higher than control group. In healthy brain, the neurotransmitters travel across the synapse but, neurodegenerative disease destroys the way electrical charges travel whithin cells [31].…”

Section: Discussionsupporting

confidence: 52%

“…In current study, the differentiated cells in the treated group connect with each other through synaptic -like connections, which may serve as the functional properties of the differentiated cells. One possible explanation for this event is component of E-CSF such as memberanous particle that have an effect on the modulation of signal transduction [17]. Hence a profitable line of future research might be to confirm this evidence such as physiological monitoring.…”

Section: Discussionmentioning

confidence: 90%

“…In sterile conditions, CSF was collected from Cisterna magna region and transferred into sterile micro tubes. After centrifugation (14,000 rpm, 5 minutes, room temperature), the supernatant was kept in –80ºC temperature [ 1 , 16 , 17 ].…”

Section: Methodsmentioning

confidence: 99%

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Role of cerebrospinal fluid in differentiation of human dental pulp stem cells into neuron-like cells

et al. 2020

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Human dental pulp stem cells (hDPSCs) could be differentiated into neuron like-cells under particular microenvironments. It has been reported that a wide range of factors, presented in cerebrospinal fluid (CSF), playing part in neuronal differentiation during embryonic stages, we herein introduce a novel culture media complex to differentiate hDPSCs into neuron-like cells. The hDPSCs were initially isolated and characterized. The CSF was prepared from the Cisterna magna of 19-day-old Wistar rat embryos, embryonic cerebrospinal fluid (E-CSF). The hDPSCs were treated by 5% E-CSF for 2 days, then neurospheres were cultured in DMEM/F12 supplemented with 10-6 μm retinoic acid (RA), glialderived neurotrophic factor and brain-derived neurotrophic factor for 6 days. The cells which were cultured in basic culture medium were considered as control group. Morphology of differentiated cells as well as process elongation were examined by an inverted microscope. In addition, the neural differentiation markers (Nestin and MAP2) were studied employing immunocytochemistry. Neuronal-like processes appeared 8 days after treatment. Neural progenitor marker (Nestin) and a mature neural marker (MAP2) were expressed in treated group. Moreover Nissl bodies were found in the cytoplasm of treated group. Taking these together, we have designed a simple protocol for generating neuron-like cells using CSF from the hDPSCs, applicable for cell therapy in several neurodegenerative disorders including Alzheimer's disease.

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Section: Discussionsupporting

confidence: 52%

Section: Discussionmentioning

confidence: 90%

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Role of cerebrospinal fluid in differentiation of human dental pulp stem cells into neuron-like cells

et al. 2020

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“…Moreover, the fact that the protein fraction of the CSF is more complex in embryos than in adults, and that it is detected at higher concentrations [10], has led some authors to suggest that the E-CSF is involved in the regulation of neuroepithelial cell behavior. In addition, analysis of E-CSF shows that different mitogenic factors with various concentrations are present in this fluid including: nerve growth factor (NGF) [8], insulin like growth factors (IGFs) [24], vascular endothelial growth factor (VEGF) [25], and transforming growth factor-β1 (TGF-β1) [26]. In this study.…”

Section: Discussionmentioning

confidence: 75%

The Effect of Chick Embryo Cerebro-Spinal Fluid in Microwave Irradiated Collagen Guide Channel on Sciatic Nerve Regeneration in Rat

Ghavami

Dolatkhah

Farjah

2016

MOJAP

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The aim of this research was to evaluate the effect of chick embryo cerebrospinal fluid (e-CSF) across the irradiated collagen guide channel on sciatic nerve regeneration in comparison with autograft. Forty adult male Sprague-Dawley rats (250-300g) were randomized into (1) collagen channel + E-CSF, (2) collagen channel + normal saline (NS), (3) autograft (AG), and (4) sham surgery groups. The left sciatic nerve was exposed and a 10mm nerve segment was cut and removed. In the collagen groups, the proximal and distal cuts ends of sciatic nerve were telescoped into the nerve guides and E-CSF or NS injected into collagen conduit. To the autograft group, the 10 mm nerve segment was turned backwards and used an autologous nerve graft. All animals were evaluated by sciatic functional index (SFI) and electrophysiology testing. The improvements in SFI since the beginning of the last evaluation in experimental groups were measured. On days 49 and 60 post-operation, the mean SFI of the collagen + E-CSF group was significantly greater than the autograft group (P< 0.05). On day 90, the mean nerve conduction velocity (NCV) of the collagen + CSF group was greater than autograft group (P< 0.05). These findings showed that RPS in collagen nerve guide channel effectively enhances nerve regeneration and promotes functional recovery in injured sciatic nerve of rats.

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“…CSF contains cytokines, retinoic acid (RA), and diffusible factors such as transforming growth factor-ß (TGF-ß), nerve growth factor (NGF), brain derived neurotrophic factor (BDNF), insulin-like growth factor, and neurotrophin-3 which can regulate the survival, proliferation, and differentiation of neuroepithelium. Neurotrophins modulate various developmental processes such as neural differentiation in the adult and developing nervous system of vertebrates (Nabiuni et al, 2015). These factors have been shown to be involved in some brain functions such as connectivity of neurons and neurite outgrowth (Yoshi et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Effects of maternal thyroid hormone deficiency on differentiation of mesenchymal stem cells in CSF-exposed neonatal Wistar rats

Mafikandi¹,

Roodbari²,

Nabiuni³

et al. 2019

Acta Neurobiologiae Experimentalis

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Cerebrospinal fluid (CSF) contains growth and neurotrophic factors which regulate proliferation, differentiation, and neurogenesis.Thyroid hormones play a crucial role in the development of the nervous system and hypothyroidism during development of embryos leads to defects in the nervous system. This study aimed to survey the effects of rat neonatal CSF collected from induced hypothyroid mothers on differentiation of bone marrow mesenchymal stem cells (BM-MSCs). We hypothesized that hypothyroidism affected levels of growth factor in CSF. To induce hypothyroidism, pregnant Wistar rats received methimazole at the third day of gestation. BM-MSCs were obtained from rat femurs and tibias and cultured in medium. CSF was collected from the cisterna magna of newborn rats, and cells were subsequently exposed to CSF with concentrations of 5,7, and 10 /100 (v/v) for 72 h.MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay and real time polymerase chain reaction (RT-PCR) were used to quantify the cell viability and analyze the expression of neural markers, respectively. Our morphological studies showed that treatment with hypothyroidism CSF (HTH-CSF) resulted in a significant decrease in neurite growth and proliferation as compared to normal CSF (N-CSF). RT-PCR analysis also showed a significant decrease in expression of neural markers (i.e., Nestin, NeuN) in cells treated with HTH-CSF as compared with the N-CSF group. The most effective concentration of CSF for BM-MSC differentiation was 5% (V/V). Our results showed a significant decrease in differentiation of BM-MSCs in the presence of neonatal CSF of hypothyroid mothers compared with neonatal CSF of healthy mothers. Thus, thyroid hormones are essential in neural development and hypothyroid defects can affect development of the neonatal brain.

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CSF Protein Contents and Their Roles in Brain Development

Cited by 4 publications

References 45 publications

Role of cerebrospinal fluid in differentiation of human dental pulp stem cells into neuron-like cells

Role of cerebrospinal fluid in differentiation of human dental pulp stem cells into neuron-like cells

The Effect of Chick Embryo Cerebro-Spinal Fluid in Microwave Irradiated Collagen Guide Channel on Sciatic Nerve Regeneration in Rat

Effects of maternal thyroid hormone deficiency on differentiation of mesenchymal stem cells in CSF-exposed neonatal Wistar rats

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