CSDR Coupling with Exo III for Ultrasensitive Electrochemistry Determination of miR-145

Abstract

Recently, miRNAs have become a promising biomarker for disease diagnostics. miRNA-145 is closely related to strokes. The accuracy determination of miRNA-145 (miR-145) in stroke patients still remains challenging due to its heterogeneity and low abundance, as well as the complexity of the blood matrix. In this work, we developed a novel electrochemical miRNA-145 biosensor via subtly coupling the cascade strand displacement reaction (CSDR), exonuclease III (Exo III), and magnetic nanoparticles (MNPs). The developed electrochemical biosensor can quantitatively detect miRNA-145 ranging from 1 × 102 to 1 × 106 aM with a detection limit as low down as 100 aM. This biosensor also exhibits excellent specificity to distinguish similar miRNA sequences even with single-base differences. It has been successfully applied to distinguish healthy people from stroke patients. The results of this biosensor are consistent with the results of the reverse transcription quantitative polymerase chain reaction (RT-qPCR). The proposed electrochemical biosensor has great potential applications for biomedical research on and clinical diagnosis of strokes.

A facile auto-combustion pathway for creating Mn and Fe-doped TiO2 nanostructures and their photocatalytic activity

“…There have been several documented techniques for making doped TiO 2 , including hydrothermal 44 , microwave heating 45 , Sol-Gel 46 , precipitation 47 , and solution combustion methods. 48,49 In some studies, the photocatalytic performance of some dyes, including amaranth 50 , crystal violet 51,52 , methyl orang 53,54 , and methylene blue 55 , was examined.…”

Label-Free Ratiometric Homogeneous Electrochemical Strategy Based on Exonuclease III-Aided Signal Amplification for Facile and Rapid Detection of miR-378

“…Electrochemical detection, which is characterized by high sensitivity, rapid response, good selectivity [13], and simplicity of operation, has been employed by researchers for microRNA detection [14][15][16][17][18][19][20]. However, conventional heterogeneous electrochemical biosensors typically involve the immobilization of a probe on a working electrode, which requires laborious and time-consuming electrode pretreatment and probe immobilization steps [21][22][23][24][25].…”