Cs
 +
 Induces the
 kdp
 Operon of
 Escherichia coli
 by Lowering the Intracellular K
 +
 Concentration

Jung, Kirsten; Krabusch, Mechthild; Altendorf, Karlheinz

doi:10.1128/jb.183.12.3800-3803.2001

Cited by 39 publications

(39 citation statements)

References 20 publications

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“…It has been proposed that the kdp expression is regulated by the internal K ϩ concentration and medium osmolarity, 5,21) but no direct evidence for this has been provided until now. Our present study provided the direct evidence to show that kdp expression is regulated by the internal K ϩ concentration.…”

Section: Discussionmentioning

confidence: 99%

Expression and Activity of Kdp under Acidic Conditions in Escherichia coli

Yan

Fukamachi

Saito

et al. 2011

Biological & Pharmaceutical Bulletin

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Escherichia coli has three major K ؉ ؉ uptake systems, Trk, Kup, and Kdp, which have been studied extensively at near neutral pH. However, the function of these transporters under acidic conditions is not well understood, although growth and survival under acidic conditions are important for bacterial pathogenesis. In this study, we examined the expression and activity of Kdp under acidic conditions and found that the transport activity of Kdp is decreased at low pH and that the expression of kdp is regulated by the internal K ؉ ؉ concentration in a pH-independent manner. Consequently, the low activity of Kdp was compensated for by the induction of its elevated expression by low K ؉ ؉ accumulation via Kdp at acidic pH.

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Section: Discussionmentioning

confidence: 99%

Expression and Activity of Kdp under Acidic Conditions in Escherichia coli

Yan

Fukamachi

Saito

et al. 2011

Biological & Pharmaceutical Bulletin

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“…This technique reveals only relative induction levels by using a radioactively labeled probe that is specific for the mRNA of interest. The protocol used for Northern analysis was described previously (21). Briefly, 20-g samples of RNA were fractionated by electrophoresis on 1.2% (wt/vol) agarose gels containing 1.1% (vol/vol) formaldehyde in MOPS (4-morpholinepropanesulfonic acid) buffer.…”

Section: Methodsmentioning

confidence: 99%

Identification of a Target Gene and Activating Stimulus for the YpdA/YpdB Histidine Kinase/Response Regulator System in Escherichia coli

Fried¹,

Behr²,

Jung³

2012

Journal of Bacteriology

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Escherichia coli contains 30 two-component systems (TCSs), each consisting of a histidine kinase and a response regulator. Whereas most TCSs are well characterized in this model organism, little is known about the YpdA/YpdB system. To identify YpdB-regulated genes, we compared the transcriptomes of E. coli cells overproducing either YpdB or a control protein. Expression levels of 15 genes differed by more than 1.9-fold between the two strains. A comprehensive evaluation of these genes identified yhjX as the sole target of YpdB. Electrophoretic mobility shift assays with purified YpdB confirmed its interaction with the yhjX promoter. Specifically, YpdB binds to two direct repeats of the motif GGCATTTCAT separated by an 11-bp spacer in the yhjX promoter. yhjX encodes a cytoplasmic membrane protein of unknown function that belongs to the major facilitator superfamily of transporters. Finally, we characterized the pattern of yhjX expression and identified extracellular pyruvate as a stimulus for the YpdA/YpdB system. It is suggested that YpdA/YpdB contributes to nutrient scavenging before entry into stationary phase.

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“…The KdpD/KdpE system controls expression of the kdpFABCDE operon, which encodes the high-affinity K ϩ transporter, as well as KdpD and KdpE (18,77). The expression of this operon is inducible by both NaCl and CsCl via different mechanisms (33). The entire kdpFABCDE operon was deleted in the KdpD/KdpE mutants.…”

Section: Vol 185 2003mentioning

confidence: 99%

Phenotype MicroArray Analysis of Escherichia coli K-12 Mutants with Deletions of All Two-Component Systems

et al. 2003

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Two-component systems are the most common mechanism of transmembrane signal transduction in bacteria. A typical system consists of a histidine kinase and a partner response regulator. The histidine kinase senses an environmental signal, which it transmits to its partner response regulator via a series of autophosphorylation, phosphotransfer, and dephosphorylation reactions. Much work has been done on particular systems, including several systems with regulatory roles in cellular physiology, communication, development, and, in the case of bacterial pathogens, the expression of genes important for virulence. We used two methods to investigate two-component regulatory systems in Escherichia coli K-12. First, we systematically constructed mutants with deletions of all two-component systems by using a now-standard technique of gene disruption (K. A. Datsenko and B. L. Wanner, Proc. Natl. Acad. Sci. USA 97:6640-6645, 2000). We then analyzed these deletion mutants with a new technology called Phenotype MicroArrays, which permits assays of nearly 2,000 growth phenotypes simultaneously. In this study we tested 100 mutants, including mutants with individual deletions of all two-component systems and several related genes, including creBC-regulated genes (cbrA and cbrBC), phoBR-regulated genes (phoA, phoH, phnCDEFGHIJKLMNOP, psiE, and ugpBAECQ), csgD, luxS, and rpoS. The results of this battery of nearly 200,000 tests provided a wealth of new information concerning many of these systems. Of 37 different two-component mutants, 22 showed altered phenotypes. Many phenotypes were expected, and several new phenotypes were also revealed. The results are discussed in terms of the biological roles and other information concerning these systems, including DNA microarray data for a large number of the same mutants. Other mutational effects are also discussed.

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Cs ⁺ Induces the kdp Operon of Escherichia coli by Lowering the Intracellular K ⁺ Concentration

Cited by 39 publications

References 20 publications

Expression and Activity of Kdp under Acidic Conditions in Escherichia coli

Expression and Activity of Kdp under Acidic Conditions in Escherichia coli

Identification of a Target Gene and Activating Stimulus for the YpdA/YpdB Histidine Kinase/Response Regulator System in Escherichia coli

Phenotype MicroArray Analysis of Escherichia coli K-12 Mutants with Deletions of All Two-Component Systems

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Cs + Induces the kdp Operon of Escherichia coli by Lowering the Intracellular K + Concentration

Cited by 39 publications

References 20 publications

Expression and Activity of Kdp under Acidic Conditions in Escherichia coli

Expression and Activity of Kdp under Acidic Conditions in Escherichia coli

Identification of a Target Gene and Activating Stimulus for the YpdA/YpdB Histidine Kinase/Response Regulator System in Escherichia coli

Phenotype MicroArray Analysis of Escherichia coli K-12 Mutants with Deletions of All Two-Component Systems

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Cs ⁺ Induces the kdp Operon of Escherichia coli by Lowering the Intracellular K ⁺ Concentration