CRZ1 transcription factor is involved in cell survival, stress tolerance, and virulence in fungi

Gupta, Surabhi; Kumar, Ajeet; Tamuli, Ranjan

doi:10.1007/s12038-022-00294-3

Cited by 5 publications

(6 citation statements)

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“…This calcineurin-CRZ1 pathway is involved in many developmental functions ranging from the cell wall synthesis, cation homeostasis, lipids metabolism, stress response in eukaryotes (Rusnak and Mertz, 2000). Like calcineurin mutants, CRZ1 deletion mutants in a variety of fungi showed sensitivity to Ca 2+ and other ionic stresses (Gupta et al, 2022). In addition, crz1 showed defects in growth, morphology, conidiation, and sclerotium formation in B. cinerea (Schumacher et al, 2008) and V. dahlia (Xiong et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

“…In this study, we gained a genome-wide perspective of the direct downstream targets of PuCRZ1 signaling using a combined approach of DAP-seq and qRT-PCR. CRZ1 in fungi have common as well as unique roles compared to those of their yeast ortholog ( Gupta et al, 2022 ; Yang et al, 2022 ). As such, we find that the suite of genes regulated by PuCRZ1 contains a high percentage specific to P. umbellatus .…”

Section: Discussionmentioning

confidence: 99%

“…In fungal cells, Calcineurin responsive Zing finger transcription factor (CRZ1) was first identified in Saccharomyces cerevisiae ( Stathopoulos and Cyert, 1997 ). Subsequently, homologs of CRZ1 gene were identified in different species ( Gupta et al, 2022 ; Yang et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

“…All CRZ1 homologs have one or more signature C2H2 zinc finger domains, and their functions and regulatory genes differ among different species ( Gupta et al, 2022 ; Yang et al, 2022 ). Crz1- regulated genes are involved in sclerotia formation in Verticillium dahliae ( Xiong et al, 2015 ) and Valsa pyri ( He et al, 2016 ), impaired mycelium growth and abnormal branching in Botrytis cinerea ( Schumacher et al, 2008 ), spores and conidia produced in B. cinerea , Metarhizium acridum ( Chen et al, 2017 ) and Fusarium graminearum ( Chen et al, 2019 ), morphological transformation, irregular plasma membrane structure and abnormal organelles in Candida lusitaniae ( Zhang et al, 2012 ).…”

Section: Introductionmentioning

confidence: 99%

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PuCRZ1, an C2H2 transcription factor from Polyporus umbellatus, positively regulates mycelium response to osmotic stress

et al. 2023

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Polyporus umbellatus is an edible and medicinal mushroom with the capacity to produce sclerotia. However, the mechanism of P. umbellatus sclerotia formation is unclear. CRZ1 is a C2H2 family transcription factor involved in the Ca2+-calcineurin signaling pathway, which has the function of regulating sclerotia formation, maintaining ion homeostasis, and responding to stress. In this study, we identified 28 C2H2 transcription factors in P. umbellatus genome, 13 of which are differentially expressed between mycelium and sclerotia, including PuCRZ1. Combining DNA affinity purification and sequencing (DAP-seq) and quantitative real-time PCR (qRT-PCR), three genes (PuG10, PuG11, PuG12) were identified as putative PuCRZ1 target genes containing a putative binding motif (GTGGCG) within their promoter. Yeast single hybridization (Y1H) and EMSA further confirmed that PuCRZ1 can bind to the promoter region of PuG10, PuG11, and PuG12. PuCRZ1 gene could reduce the sensitivity of NaCl in yeast cells. Furthermore, overexpression of the PuCRZ1 target gene, especially the FVLY domain containing gene PuG11, could improve the mycelia growth rate and mannitol tolerance in P. umbellatus. These results demonstrate that PuCRZ1 in the Ca2+-calcineurin signaling pathway plays an important role in mycelia growth, as well as osmotic stress tolerance.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

PuCRZ1, an C2H2 transcription factor from Polyporus umbellatus, positively regulates mycelium response to osmotic stress

et al. 2023

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“…The development of these structures and the infection in plants is inhibited by cyclosporin (Viaud et al, 2002(Viaud et al, , 2003Schumacher et al, 2008). In these infections, calcium regulates growth rate, morphology, differentiation of the filamentous fungus and attachment and penetration in the plant (Gupta et al, 2022). This is a very complex phenomenon that requires many enzymes, and bioactive metabolites that play important roles in intercellular communications (Table 2), however, in only a few fungal infections there is clear evidence of the involvement of calcineurin regulation in secondary metabolites production.…”

Section: Calcineurin In Fungal Pathogenesis Related To Production Of ...mentioning

confidence: 99%

Interaction of calcium responsive proteins and transcriptional factors with the PHO regulon in yeasts and fungi

Martı́n

2023

Front. Cell Dev. Biol.

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Phosphate and calcium ions are nutrients that play key roles in growth, differentiation and the production of bioactive secondary metabolites in filamentous fungi. Phosphate concentration regulates the biosynthesis of hundreds of fungal metabolites. The central mechanisms of phosphate transport and regulation, mediated by the master Pho4 transcriptional factor are known, but many aspects of the control of gene expression need further research. High ATP concentration in the cells leads to inositol pyrophosphate molecules formation, such as IP3 and IP7, that act as phosphorylation status reporters. Calcium ions are intracellular messengers in eukaryotic organisms and calcium homeostasis follows elaborated patterns in response to different nutritional and environmental factors, including cross-talking with phosphate concentrations. A large part of the intracellular calcium is stored in vacuoles and other organelles forming complexes with polyphosphate. The free cytosolic calcium concentration is maintained by transport from the external medium or by release from the store organelles through calcium permeable transient receptor potential (TFP) ion channels. Calcium ions, particularly the free cytosolic calcium levels, control the biosynthesis of fungal metabolites by two mechanisms, 1) direct interaction of calcium-bound calmodulin with antibiotic synthesizing enzymes, and 2) by the calmodulin-calcineurin signaling cascade. Control of very different secondary metabolites, including pathogenicity determinants, are mediated by calcium through the Crz1 factor. Several interactions between calcium homeostasis and phosphate have been demonstrated in the last decade: 1) The inositol pyrophosphate IP3 triggers the release of calcium ions from internal stores into the cytosol, 2) Expression of the high affinity phosphate transporter Pho89, a Na+/phosphate symporter, is controlled by Crz1. Also, mutants defective in the calcium permeable TRPCa7-like of Saccharomyces cerevisiae shown impaired expression of Pho89. This information suggests that CrzA and Pho89 play key roles in the interaction of phosphate and calcium regulatory pathways, 3) Finally, acidocalcisomes organelles have been found in mycorrhiza and in some melanin producing fungi that show similar characteristics as protozoa calcisomes. In these organelles there is a close interaction between orthophosphate, pyrophosphate and polyphosphate and calcium ions that are absorbed in the polyanionic polyphosphate matrix. These advances open new perspectives for the control of fungal metabolism.

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The CRZ-1 transcription factor binds to specific sequences in the promoters of al-2, cao-2, and ylo-1 to regulate the expression of these carotenoid biosynthesis genes in Neurospora crassa

Kumar,

Tamuli

2024

Mycol Progress

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CRZ1 transcription factor is involved in cell survival, stress tolerance, and virulence in fungi

Cited by 5 publications

References 141 publications

PuCRZ1, an C2H2 transcription factor from Polyporus umbellatus, positively regulates mycelium response to osmotic stress

PuCRZ1, an C2H2 transcription factor from Polyporus umbellatus, positively regulates mycelium response to osmotic stress

Interaction of calcium responsive proteins and transcriptional factors with the PHO regulon in yeasts and fungi

The CRZ-1 transcription factor binds to specific sequences in the promoters of al-2, cao-2, and ylo-1 to regulate the expression of these carotenoid biosynthesis genes in Neurospora crassa

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