To investigate mechanical properties of Ti6321 titanium alloy, tensile tests at different strain rates are carried out. The changes of microstructure and properties at the range of 10−3 s−1–105 s−1 have been studied. Lower density and larger size of dimples produce when stretched at high strain rates of 103 s−1 compared to quasi-static stretching. The yield strengths of the alloy are 805, 1096 and 2440 MPa at strain rates of 10−3 s−1, 2 × 103 s−1 and 105 s−1, respectively with obvious strain rate strengthening effect. At 105 s−1 strain rate, parallel twins generate at grain boundaries to coordinate the deformation when dislocations cannot meet indicating the twinning mechanism comes into play.