Abstract:The crystal structure of a subtilisin-like serine proteinase from the psychrotrophic marine bacterium, Vibrio sp. PA-44, was solved by means of molecular replacement and refined at 1.84 Å resolution. This is the first structure of a cold adapted subtilase to be determined and its elucidation facilitates examination of the molecular principles underlying temperature adaptation of enzymes. The cold adapted Vibrio-proteinase was compared to known three-dimensional structures of homologous enzymes of meso-and ther… Show more
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