Crystallization studies of the keratin-like domain from<i>Arabidopsis thaliana</i>SEPALLATA 3

Acajjaoui, Samira; Zubieta, Chloé

doi:10.1107/s174430911302006x

Cited by 5 publications

(3 citation statements)

References 39 publications

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“…The construct comprising residues 75 to 178 (SEP3 75-178 ) was selected for further studies (Acajjaoui and Zubieta, 2013) (Figure 1B). This construct contained the complete K domain (91 to 173) and overlapped a portion of the I domain (residues 75 to 90) and the C domain (residues 174 to 178).…”

Section: Resultsmentioning

confidence: 99%

Structural Basis for the Oligomerization of the MADS Domain Transcription Factor SEPALLATA3 in Arabidopsis

et al. 2014

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In plants, MADS domain transcription factors act as central regulators of diverse developmental pathways. In Arabidopsis thaliana, one of the most central members of this family is SEPALLATA3 (SEP3), which is involved in many aspects of plant reproduction, including floral meristem and floral organ development. SEP3 has been shown to form homo and heterooligomeric complexes with other MADS domain transcription factors through its intervening (I) and keratin-like (K) domains. SEP3 function depends on its ability to form specific protein-protein complexes; however, the atomic level determinants of oligomerization are poorly understood. Here, we report the 2.5-Å crystal structure of a small portion of the intervening and the complete keratin-like domain of SEP3. The domains form two amphipathic alpha helices separated by a rigid kink, which prevents intramolecular association and presents separate dimerization and tetramerization interfaces comprising predominantly hydrophobic patches. Mutations to the tetramerization interface demonstrate the importance of highly conserved hydrophobic residues for tetramer stability. Atomic force microscopy was used to show SEP3-DNA interactions and the role of oligomerization in DNA binding and conformation. Based on these data, the oligomerization patterns of the larger family of MADS domain transcription factors can be predicted and manipulated based on the primary sequence.

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Section: Resultsmentioning

confidence: 99%

Structural Basis for the Oligomerization of the MADS Domain Transcription Factor SEPALLATA3 in Arabidopsis

et al. 2014

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“…These differences can further be attributed to the differences in structure. Although, crystallization studies would be ideal for these kind of studies, except SEPALLATA3 none of the Arabidopsis MADS box proteins have been crystallized (Acajjaoui and Zubieta 2013;Puranik et al 2014), largely due to difficulties in isolating high-quality protein in vitro.…”

Section: Discussionmentioning

confidence: 99%

C-terminal domain of APETALA1 is essential for its functional divergence from CAULIFLOWER in Arabidopsis

Saini

Yadav

2020

J. Plant Biochem. Biotechnol.

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APETALA1 (AP1) and CAULIFLOWER (CAL) are involved in floral meristem identity and suppress the inflorescence meristem program in flower meristem in Arabidopsis thaliana. Based on the ap1 mutant phenotype genetic studies demonstrated that it is involved in sepal and petal identity specification as well as bract suppression in flower. Despite having high sequence similarity with AP1, its closest paralog CAL does not show a defect in flower development. In ap1 cal1 double mutant, floral meristem turns into inflorescence meristem resulting in a cauliflower like phenotype. cal mutants appear like wild type, suggesting that AP1 compensates its function. Here, we report that AP1 can replace CAL by interacting its potential interactors. Functional studies using the truncated version of AP1 confirmed that the C-terminal domain of AP1 is exclusively involved in floral organ identity specification.Transcription factors PPIs Protein-protein interactions Y2H Yeast-two-hybrid Electronic supplementary material The online version of this article (

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“…It is expressed in all tissues, and is abundantly expressed in apical meristem, pollen and tapetum [40]. ACS9 has been confirmed to be expressed in stigma [41]. In higher plants, the MADS-box genes encode a large family of transcription factors (TFs) involved in key developmental processes, most notably plant reproduction, flowering and floral organ development.…”

Section: Plos Onementioning

confidence: 99%

Transcriptome sequencing and screening of genes related to sex determination of Trichosanthes kirilowii Maxim

Liao

Zhang

et al. 2020

PLoS ONE

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Trichosanthes kirilowii Maxim. (TK) is a dioecious plant in the Cucurbitaceae for which different sexes have separate medicinal uses. In order to study the genes related to sex determination, transcriptome sequencing was performed on flower buds of male and female plants using the high-throughput sequencing technology. A total of 145,975 unigenes and 7110 DEGs were obtained. There were 6776 DEGs annotated to 1234 GO terms and enriched to 18 functional groups, including five biological processes related to sugar metabolism. KEGG pathway analysis indicated genes involved in hormone transduction, hormone synthesis and carbohydrate metabolism. Many DEGs of TK are involved in reproductive organ formation, hormone signal transduction and regulatory networks. Combining the results of GO, KEGG and qRT-PCR, 11 sex determining candidate genes of TK were selected, including MYB80,

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Crystallization studies of the keratin-like domain fromArabidopsis thalianaSEPALLATA 3

Cited by 5 publications

References 39 publications

Structural Basis for the Oligomerization of the MADS Domain Transcription Factor SEPALLATA3 in Arabidopsis

Structural Basis for the Oligomerization of the MADS Domain Transcription Factor SEPALLATA3 in Arabidopsis

C-terminal domain of APETALA1 is essential for its functional divergence from CAULIFLOWER in Arabidopsis

Transcriptome sequencing and screening of genes related to sex determination of Trichosanthes kirilowii Maxim

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