Crystallization of Purines in the Vacuole of Candida utilis

Roush, Allan H.

doi:10.1038/190449a0

Cited by 31 publications

(12 citation statements)

References 5 publications

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“…Accumulation of these compounds depends on the yeast species, the growth conditions and the stage of growth. The vacuoles of the yeast Candida utilis contain crystals of ureic acid (Roush, 1961), adenine, isoguanine (Svihla et al, 1963) and S-adenosyl-Lmethionine (Svihla and Schlenk, 1960;Nakamura, 1973). The data on the size of the vacuolar pools of nucleosides are very scarce.…”

Section: Discussionmentioning

confidence: 99%

Transport of organic acid anions and guanosine into vacuoles of Saccharomyces pastorianus

Kulakovskaya

Matys

Okorokov

1991

Yeast

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Isolated vacuoles of the yeast Saccharomyces pastorianus accumulate citrate, α‐ketoglutarate, malate and guanosine. This accumulation is Mg ATP‐dependent and inhibited by protonophores. The ionophores monensin and A23187 (electroneutral Men+/nH+‐exchange) inhibit guanosine accumulation but fail to block citrate uptake. Mg2+ ions (2 mM) increase the values of both Δ\documentclass{article}\pagestyle{empty}\begin{document}$ \tilde \mu $\end{document}H+ components and stimulate the uptake of all the above compounds. Ca2+ ions (1 mM), hyperpolarizing the yeast vacuolar membrane and dissipating the pH gradient, inhibit guanosine uptake and stimulate that of citrate. It is concluded that guanosine is transported into yeast vacuoles by an H+/guanosine antiporter while citrate, malate and α‐ketoglutarate are translocated by a uniporter(s) at the expense of the membrane potential (positive inside).

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Section: Discussionmentioning

confidence: 99%

Transport of organic acid anions and guanosine into vacuoles of Saccharomyces pastorianus

Kulakovskaya

Matys

Okorokov

1991

Yeast

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“…More recently, Roush, Questiaux, and Domnas (1959) published an elaborate study of the uptake of purine bases by C. utilis. Roush (1961) observed crystals of various purines in the vacuole by conventional microscopy. This gave further encouragement to our cytological study.…”

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confidence: 99%

Ultraviolet Microscopy of Purine Compounds in the Yeast Vacuole

1963

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Ultraviolet microscopy of purine compounds in the yeast vacuole. J. Bacteriol. 85:399-409. 1962.-Yeast cells (Candida utilis and Saccharomyces cerevisiae) suspended in nitrogen-free medium were exposed to various ultravioletabsorbing biological compounds, particularly nucleic acid constituents. Ultraviolet photomicrography was used to locate these substances in the cells. Purines were taken up readily and concentrated in the vacuoles of C. utilis but not of S. cerevisiae. Crystallization occurred, as observed earlier by other techniques. Neither organism assimilated pyrimidine bases, or purine or pyrimidine nucleosides, at a detectable rate. From the selective uptake and release of some purine derivatives, it can be concluded that the properties of the vacuolar membrane and the cytoplasmic membrane differ in several respects. MATERIALS AND METHODS C. utilis ATCC 9950 and S. cerevisiae ATCC 7752 were cultured as specified earlier (Svihla, Schlenk, and Dainko, 1960, 1961). The washed cells were stored at 4 C until use. For experiments with the purines, pyrimidines, and other ultraviolet-absorbing compounds, the same salt medium was used without ammonium sulfate. Glu-399

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“…Some yeasts cannot assimilate the nitrogen of heterocyclic compounds (2,5). In others, induced enzymes (7,8,9) gradually provide nitrogen for metabolic purposes by breakdown of thepurine ring system, and a temporary nitrogen deficiency may prevail in the early phases of the culture.…”

Section: Figmentioning

confidence: 99%

Ultraviolet Microscopy of Purines and Amino Acids in the Vacuole of Candida albicans

Balish

Svihla

1968

J Bacteriol

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Ultraviolet (UV) microscopy was used to study the capacity of yeast (ATCC 10231 and 10261) and filamentous (ATCC 10259) strains of Candida albicans to accumulate UV-absorbing materials from a medium supplemented with purines, pyrimidines, amino acids, or related compounds as the main nitrogen source. All strains accumulated UV-absorbing compounds when adenine, adenosine, isoguanine, xanthine, or uric acid was supplied as a nitrogen source, but they did not accumulate UV-absorbing compounds when pyrimidines were supplied. The filamentous strain accumulated UV-absorbing material from medium supplemented with hypoxanthine, but the yeast strains did not. In contrast, the yeast strains accumulated more UV-absorbing material than did the filamentous strain when guanine was the nitrogen source. Yeast strain 10231 not only accumulated UVabsorbing material from tyrosine-supplemented medium, but it became filamentous in form as well. Yeast strain 10261 and filamentous strain 10259 did not accumulate detectable amounts of UV-absorbing material, nor was their morphology noticeably affected by the supplement. The two yeast strains accumulated more lipid than the filamentous strain when they were incubated in a nitrogen-deficient medium.

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Crystallization of Purines in the Vacuole of Candida utilis

Cited by 31 publications

References 5 publications

Transport of organic acid anions and guanosine into vacuoles of Saccharomyces pastorianus

Transport of organic acid anions and guanosine into vacuoles of Saccharomyces pastorianus

Ultraviolet Microscopy of Purine Compounds in the Yeast Vacuole

Ultraviolet Microscopy of Purines and Amino Acids in the Vacuole of Candida albicans

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