Crystallization of porcine insulin with carbon dioxide as acidifying agent

Hirata, Gisele Atsuko Medeiros; Bernardo, André; Miranda, Everson Alves

doi:10.1016/j.powtec.2009.08.017

Cited by 9 publications

(6 citation statements)

References 14 publications

(15 reference statements)

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“…The use of volatile electrolytes such as CO 2 , as an alternative to the use of acids in protein precipitation, overcomes these disadvantages and adds additional benefits to the process, such as preventing protein denaturation caused by localised pH extremes and greatly reduced saline effluent generation. CO 2 can be easily separated by pressure release and economically recycled for multiple precipitation cycles [395]. The mild acidic and anti-solvent properties of the liquid and supercritical CO 2 have been shown to aid the separation and purification of food proteins.…”

Section: Ultrasound and Supercritical Carbon Dioxide (Scrco 2 ) As Prmentioning

confidence: 99%

“…Strong interactions with various proteins in both aqueous [396,397] and organic solutions [398] of SCrCO 2 has been demonstrated, triggering the formation of protein particles and/or the precipitation of selected proteins via acidification or anti-solvent effects. These properties have been utilized to extract, isolate, or purify diverse types of proteins from different sources such as soybeans [399][400][401], milk [402,403], fish [404,405], fruits [406], porcine insulin [395], or model proteins [407]. The solubility of SCrCO 2 in protein solutions and the diverse effects of SCrCO 2 on the protein structure and pH have been studied [408][409][410][411].…”

Section: Ultrasound and Supercritical Carbon Dioxide (Scrco 2 ) As Prmentioning

confidence: 99%

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Protein Recovery from Underutilised Marine Bioresources for Product Development with Nutraceutical and Pharmaceutical Bioactivities

2020

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The global demand for dietary proteins and protein-derived products are projected to dramatically increase which cannot be met using traditional protein sources. Seafood processing by-products (SPBs) and microalgae are promising resources that can fill the demand gap for proteins and protein derivatives. Globally, 32 million tonnes of SPBs are estimated to be produced annually which represents an inexpensive resource for protein recovery while technical advantages in microalgal biomass production would yield secure protein supplies with minimal competition for arable land and freshwater resources. Moreover, these biomaterials are a rich source of proteins with high nutritional quality while protein hydrolysates and biopeptides derived from these marine proteins possess several useful bioactivities for commercial applications in multiple industries. Efficient utilisation of these marine biomaterials for protein recovery would not only supplement global demand and save natural bioresources but would also successfully address the financial and environmental burdens of biowaste, paving the way for greener production and a circular economy. This comprehensive review analyses the potential of using SPBs and microalgae for protein recovery and production critically assessing the feasibility of current and emerging technologies used for the process development. Nutritional quality, functionalities, and bioactivities of the extracted proteins and derived products together with their potential applications for commercial product development are also systematically summarised and discussed.

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Section: Ultrasound and Supercritical Carbon Dioxide (Scrco 2 ) As Prmentioning

confidence: 99%

Section: Ultrasound and Supercritical Carbon Dioxide (Scrco 2 ) As Prmentioning

confidence: 99%

Protein Recovery from Underutilised Marine Bioresources for Product Development with Nutraceutical and Pharmaceutical Bioactivities

2020

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“…Kwon et al manipulated a method to produce insulin crystals with a size of less than 5 μm. Carbon dioxide can be used as an acidifying agent to produce small insulin crystals with a size of less than 10 μm in a stirred tank . Such a process benefits from the slow addition of carbon dioxide to avoid extreme gradients in the pH, and the type of mixing can significantly affect the crystal size distribution (CSD) .…”

Section: Introductionmentioning

confidence: 99%

“…Carbon dioxide can be used as an acidifying agent to produce small insulin crystals with a size of less than 10 μm in a stirred tank . Such a process benefits from the slow addition of carbon dioxide to avoid extreme gradients in the pH, and the type of mixing can significantly affect the crystal size distribution (CSD) . In general, mixing conditions have to be chosen carefully, because protein stability and crystallization kinetics are sensitive to shear forces. , Control over shear forces is generally challenging in scaling up crystallizers that resemble well-mixed tanks.…”

Section: Introductionmentioning

confidence: 99%

Integrated Continuous Crystallization and Spray Drying of Insulin for Pulmonary Drug Delivery

Chen

Weng

Chow

et al. 2020

Crystal Growth & Design

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Pulmonary delivery of insulin in the form of a dry powder is an attractive alternative in comparison to conventional subcutaneous delivery. Insulin crystals have potential advantages such as sustained release and higher stability in comparison to amorphous particles. However, the design of crystallization processes that can reliably produce insulin crystals in a suitable size range for pulmonary delivery is challenging. A continuous process involving the integration of a tubular crystallizer and a spray dryer is characterized for the production of inhalable insulin. Supersaturation is created via cooling and a pH shift at the inlet of the crystallizer, aiming at a high nucleation rate. The recovery of insulin in the crystallizer exceeds 90%, and the insulin particles appear crystalline under most of the tested conditions. Integration with a spray dryer produces a dry powder with favorable properties for pulmonary drug delivery. The emitted fraction of insulin from a capsule in a commercial inhaler is in the range of 84.3−93.1%, which shows good powder dispersibility. Furthermore, the fine particle fraction of the insulin crystals ranges from 22.0% to 36.4%, which indicates a good potential for pulmonary delivery.

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“…sCO 2 has demonstrated strong interactions with various proteins both in aqueous [ 16 , 17 ] and organic solutions [ 18 ], triggering the formation of protein particles and/or the precipitation of select proteins out of solution via acidification or anti-solvent effects. These phenomena have been utilized to extract, isolate or purify diverse types of proteins such as soy proteins [ 19 – 23 ], fish proteins [ 24 , 25 ], fruit proteins [ 26 ], porcine insulin [ 27 ] or model protein [ 28 ].…”

Section: Introductionmentioning

confidence: 99%

Fractionation of Whey Protein Isolate with Supercritical Carbon Dioxide—Process Modeling and Cost Estimation

Yver

Bonnaillie

Yee

et al. 2011

IJMS

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An economical and environmentally friendly whey protein fractionation process was developed using supercritical carbon dioxide (sCO2) as an acid to produce enriched fractions of α-lactalbumin (α-LA) and β-lactoglobulin (β-LG) from a commercial whey protein isolate (WPI) containing 20% α-LA and 55% β-LG, through selective precipitation of α-LA. Pilot-scale experiments were performed around the optimal parameter range (T = 60 to 65 °C, P = 8 to 31 MPa, C = 5 to 15% (w/w) WPI) to quantify the recovery rates of the individual proteins and the compositions of both fractions as a function of processing conditions. Mass balances were calculated in a process flow-sheet to design a large-scale, semi-continuous process model using SuperproDesigner® software. Total startup and production costs were estimated as a function of processing parameters, product yield and purity. Temperature, T, pressure, P, and concentration, C, showed conflicting effects on equipment costs and the individual precipitation rates of the two proteins, affecting the quantity, quality, and production cost of the fractions considerably. The highest α-LA purity, 61%, with 80% α-LA recovery in the solid fraction, was obtained at T = 60 °C, C = 5% WPI, P = 8.3 MPa, with a production cost of $8.65 per kilogram of WPI treated. The most profitable conditions resulted in 57%-pure α-LA, with 71% α-LA recovery in the solid fraction and 89% β-LG recovery in the soluble fraction, and production cost of $5.43 per kilogram of WPI treated at T = 62 °C, C = 10% WPI and P = 5.5 MPa. The two fractions are ready-to-use, new food ingredients with a pH of 6.7 and contain no residual acid or chemical contaminants.

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Crystallization of porcine insulin with carbon dioxide as acidifying agent

Cited by 9 publications

References 14 publications

Protein Recovery from Underutilised Marine Bioresources for Product Development with Nutraceutical and Pharmaceutical Bioactivities

Protein Recovery from Underutilised Marine Bioresources for Product Development with Nutraceutical and Pharmaceutical Bioactivities

Integrated Continuous Crystallization and Spray Drying of Insulin for Pulmonary Drug Delivery

Fractionation of Whey Protein Isolate with Supercritical Carbon Dioxide—Process Modeling and Cost Estimation

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