Crystallization of lysozyme: From vapor diffusion experiments to batch crystallization in agitated ml-scale vessels

Hekmat, Dariusch; Hebel, Dirk; Schmid, Hans‐Joachim; Weuster‐Botz, Dirk

doi:10.1016/j.procbio.2007.10.001

Cited by 56 publications

(57 citation statements)

References 18 publications

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“…In other research, it was shown that the addition of a fluorinated pyrrolidinium-based IL to an aqueous lysozyme solution (without conventional crystallization agents) led to the formation of crystals [6]. Elsewhere, it was shown that the addition of the biocompatible IL, ethano-lammonium formate, to an aqueous solution containing a conventional crystallization agent in a mL-scale stirred crystallizer clearly led to larger lysozyme crystals compared to the solution without the IL [7].…”

Section: Introductionmentioning

confidence: 99%

Crystalline Proteins as an Alternative to Standard Formulations

2008

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Crystalline proteins may offer superior properties for drug delivery compared to standard protein formulations such as aqueous solutions or amorphous precipitated lyophilisates. In this study, a new approach using biocompatible, hydrophilic, substituted alkylammonium-based ionic liquids (ILs) as additives for the advanced crystallization of two exemplary proteins, lysozyme and lipase, was investigated. Sitting-drop vapor diffusion crystallization experiments revealed that the addition of some of the ILs resulted in less crystal polymorphism and precipitation was consistently avoided, even at larger concentrations of the conventional crystallization agent. The kinetics of lysozyme crystallization were significantly enhanced by a factor of up to 5.5 using ILs with strongly hydrated anions, i.e., formate or glycolate. In contrast, ILs with weakly hydrated anions, i.e., nitrate, led to undesirable spontaneous precipitation. In addition, lipase was crystallized preferentially using an IL with a strongly hydrated anion, i.e. dihydrogenphosphate. Large, sturdy crystals were formed at rates which were enhanced by a factor of up to 4.

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Section: Introductionmentioning

confidence: 99%

Crystalline Proteins as an Alternative to Standard Formulations

2008

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“…While batch crystallization has its process advantages, successful transfer of an existing vapor diffusion-based crystallization method to microbatch and then its scale-up to process level remains a challenging task. Hekmat and coworkers describe the successful transfer of vapor diffusion-based crystallization to agitated batch crystallization at milliliter scale using lysozyme as a model protein [19]. The same research group also describes a stirred-tank crystallization-based approach [53] towards successful scale-up of batch crystallization conditions using lysozyme, lipase [18], and therapeutic mAbs [17,52] as model proteins.…”

Section: Protein Crystallography: Principle History and Applicationsmentioning

confidence: 99%

Current Trends and Advances in Bulk Crystallization and Freeze-Drying of Biopharmaceuticals

Gutka

Prasad

2015

Lyophilized Biologics and Vaccines

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“…Crystallization conditions were adapted from Hekmat et al [10]. Firstly, the lysozyme is dissolved into a 25 mM sodium acetate buffer at a pH of 4.…”

Section: Crystallization and Detection Of Crystal Size Distribution (mentioning

confidence: 99%

Mechanical Stress on Lysozyme Crystals during Dynamic Cross‐Flow Filtration

2013

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The influence of mechanical stress on the crystal size distribution of lysozyme crystal slurries in a dynamic cross-flow device is discussed. The reduction of crystal size may have an influence on mass and resistance of the fouling layer. A filter test device with a filter area of 130 cm 2 is used for thickening. After thickening, a model impurity, namely bovine serum albumin, is washed out of the suspension. A reduction of crystal size due to stirring and a reduced fouling layer with increasing stirrer speed is observed. Furthermore, a higher stirring speed increases the specific filtrate flux. In the presence of the impurity, more crystal aggregates than single isometric crystals are formed and, hence, the decrease of crystal size due to shear forces is more pronounced than without impurity.

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Crystallization of lysozyme: From vapor diffusion experiments to batch crystallization in agitated ml-scale vessels

Cited by 56 publications

References 18 publications

Crystalline Proteins as an Alternative to Standard Formulations

Crystalline Proteins as an Alternative to Standard Formulations

Current Trends and Advances in Bulk Crystallization and Freeze-Drying of Biopharmaceuticals

Mechanical Stress on Lysozyme Crystals during Dynamic Cross‐Flow Filtration

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