Crystallization of calcium phosphates. A constant composition study

Koutsoukos, Petros G.; Amjad, Zahid; Tomson, Mason B.; Nancollas, G.H.

doi:10.1021/ja00525a015

Cited by 392 publications

(249 citation statements)

References 3 publications

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“…The pH was measured using a combined glass/Ag/AgCl electrode (Metrohm, 6.0202.100), standardized before and after each experiment with National Bureau of Standards (NBS) standard buffer solutions. The HAP seed crystals were prepared from solutions of Ca(NO 3 ) 2 , H 3 PO 4 , and NH 3 , aged in the mother liquor for 8 months, filtered, and dried as described previously (39). Their specific surface area was found to be 34.6 m 2 /g as determined by a multiple point Brunauer, Emmett, and Teller (BET) method (PerkinElmer Life Sciences, Sorptometer 212D).…”

Section: Methodsmentioning

confidence: 99%

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Serglycin Constitutively Secreted by Myeloma Plasma Cells Is a Potent Inhibitor of Bone Mineralization in Vitro

Theocharis

Seidel

Børset

et al. 2006

Journal of Biological Chemistry

115

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Although the biological significance of proteoglycans (PGs) has previously been highlighted in multiple myeloma (MM), little is known about serglycin, which is a hematopoietic cell granule PG. In this study, we describe the expression and highly constitutive secretion of serglycin in several MM cell lines. Serglycin messenger RNA was detected in six MM cell lines. PGs were purified from conditioned medium of four MM cell lines, and serglycin substituted with 4-sulfated chondroitin sulfate was identified as the predominant PG. Flow cytometry and confocal microscopy showed that serglycin was also present intracellularly and on the cell surface, and attachment to the cell surface was at least in part dependent on intact glycosaminoglycan side chains. Immunohistochemical staining of bone marrow biopsies showed the presence of serglycin both in benign and malignant plasma cells. Immunoblotting in bone marrow aspirates from a limited number of patients with newly diagnosed MM revealed highly increased levels of serglycin in 30% of the cases. Serglycin isolated from myeloma plasma cells was found to influence the bone mineralization process through inhibition of the crystal growth rate of hydroxyapatite. This rate reduction was attributed to adsorption and further blocking of the active growth sites on the crystal surface. The apparent order of the crystallization reaction was found to be n ‫؍‬ 2, suggesting a surface diffusion-controlled spiral growth mechanism. Our findings suggest that serglycin release is a constitutive process, which may be of fundamental biological importance in the study of MM.

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Section: Methodsmentioning

confidence: 99%

“…the number of moles of all the reactants was maintained constant during the reaction) (39). Experimental conditions were chosen to physicochemically resemble the biological ones (i.e.…”

Section: Methodsmentioning

confidence: 99%

Serglycin Constitutively Secreted by Myeloma Plasma Cells Is a Potent Inhibitor of Bone Mineralization in Vitro

Theocharis

Seidel

Børset

et al. 2006

Journal of Biological Chemistry

115

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“…The details are available elsewhere (Dorozhkin et al 2004) but, briefly, the first problem might be overcome by either a continuous flow of a supersaturated solution Vallet-Regí et al 2000) or using a constantcomposition (CC) technique (Nancollas and Wu 2000;Koutsoukos et al 1980;Tomson and Nancollas 1978). The second difference might be surpassed by a restrained diffusion of calcium and orthophosphate ions from the opposite directions in, for example, a double-diffusion (DD) crystallization device or in viscous gels (Busch et al 1999;Manjubala et al 2006;Cai et al 2010;Yokoi et al 2010;Sadjadi et al 2010).…”

Section: Biomimetic Crystallization Of Capomentioning

confidence: 99%

Calcium orthophosphates (CaPO 4 ): Occurrence and properties

Dorozhkin¹

2017

Morphologie

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“…Nearly constant supersaturation was achieved via simultaneous inflow of equimolar CaCl 2 and Na 2 SO 4 solutions that compensated for the precipitation of crystal lattice constituents, i.e. Ca and SO 4 as described in [36]. This approach distinguishes the present study from previous batch type growth research involving α-HH [29].…”

Section: Introductionmentioning

confidence: 99%

The crystal growth kinetics of alpha calcium sulfate hemihydrate in concentrated CaCl2–HCl solutions

Feldmann

Demopoulos²

2012

Journal of Crystal Growth

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The crystal growth kinetics of calcium sulfate α-hemihydrate (α-HH) in nearly constant supersaturated HCl-CaCl 2 solutions were investigated. Two types of solutions were used, the first had a low HCl (1.4 mol/L) and high CaCl 2 (2.8 mol/L) concentration and the second had a high HCl (5.6 mol/L) and low CaCl 2 (0.7 mol/L) concentration. These conditions were chosen to represent the first and last stage of a newly developed stage-wise HCl regeneration process. The seeded growth experiments were carried out in a stirred, temperature controlled semi-batch reactor in which supersaturation was kept constant by simultaneous addition of CaCl 2 and Na 2 SO 4 solutions. The influence of the following parameters on α-HH crystal growth was studied: temperature (70-95°C), specific power input of stirring (0.02-1.29 W/kg) and equimolar inflow rate of CaCl 2 and Na 2 SO 4 (0-0.6 mol/h). The crystal growth rate was derived from particle size distribution measurements made with the laser light diffraction technique. It was found that the surface area normalized crystal growth rate increased linearly with the molar inflow rate up to 0.3 mol/h, at higher inflow rates no further increase of the growth rate was observed. Temperature and specific power input, within the investigated ranges, did not show a marked effect on the growth rate, attributable to a diffusion/adsorption controlled growth process. An interesting finding of the present research is the establishment of a positive relationship between the narrowing of the width of the particle size distribution with increasing crystal growth rate. The results show that the resulting particle size distribution is positively related to the reagent inflow rate, a finding that can be applied to the industrial design and scale-up of the α-HH crystallization/HCl regeneration process.

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Crystallization of calcium phosphates. A constant composition study

Cited by 392 publications

References 3 publications

Serglycin Constitutively Secreted by Myeloma Plasma Cells Is a Potent Inhibitor of Bone Mineralization in Vitro

Serglycin Constitutively Secreted by Myeloma Plasma Cells Is a Potent Inhibitor of Bone Mineralization in Vitro

Calcium orthophosphates (CaPO 4 ): Occurrence and properties

The crystal growth kinetics of alpha calcium sulfate hemihydrate in concentrated CaCl2–HCl solutions

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