Crystallization and preliminary X-ray crystallographic analysis of the XoGroEL chaperonin from<i>Xanthomonas oryzae</i>pv.<i>oryzae</i>

Tran, Huyen Thi Thanh; Pham, Tan‐Viet; Ngo, H.P.T.; Hong, Myoung Ki; Kim, Jeong Gu; Lee, Sang Hee; Ahn, Yeh Jin; Kang, Lin Woo

doi:10.1107/s2053230x14006591

Cited by 5 publications

(3 citation statements)

References 27 publications

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“…The ability of bacteria to effectively degrade OTC is said to be associated with enzyme productions such as manganese peroxidase [ 59 ], laccase [ 60 ], glutathione S-transferases [ 61 ], and tetracycline inactivating monooxygenase [ 62 – 64 ]. Hence, the degradation of OTC by Lysinibacillus 3+I can be considered to share a similar degradation process.…”

Section: Discussionmentioning

confidence: 99%

Oxytetracycline Degrading Potential of Lysinibacillus sp. Strain 3+I Isolated from Poultry Manure

Sudha

Parthasarathi

Prabha

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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Oxytetracycline (OTC) which is a broad-spectrum veterinary tetracycline antibiotic is extensively used in poultry farms as a prophylactic, therapeutic, and growth stimulator. Upon administration, unmetabolized OTC is excreted from the animal body through droppings and accumulated in litter in the poultry industry. This study aimed at investigating the OTC degradation potential of an-OTC tolerant bacterial strain, isolated from poultry manure. The isolated strain’s morphology, biochemical properties, and 16S ribosomal RNA (rRNA) gene sequence confirmed that it belonged to the Lysinibacillus genus. To measure the residual OTC concentration, a high-performance liquid chromatography method was used. OTC degradation rates were 2.579 mg L−1d−1 with Lysinibacillus strain 3+I and 1.149 mg L−1d−1 without Lysinibacillus strain 3+I. In the presence of strain 3+I, the half-life significantly reduced to 2.68 days, compared to 6.03 days without strain 3+I. The strain demonstrated 85% removal with the OTC concentration of 10 μg/ml. The influence of pH, temperature, carbon sources, and nitrogen source, which influence degradation, were also investigated. The optimum condition favouring degradation was pH 6 at a temperature of 30°C. In addition, Lysinibacillus sp. strain 3+I’s ability to degrade OTC in poultry litter offers a promising approach to treat poultry manure and effluent containing OTC, preventing its contamination in the environment.

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Section: Discussionmentioning

confidence: 99%

Oxytetracycline Degrading Potential of Lysinibacillus sp. Strain 3+I Isolated from Poultry Manure

Sudha

Parthasarathi

Prabha

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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“…Gene cloning and protein expression and purification were performed as described in [10]. Basically, the XoGroEL (Xoo4288)-coding DNA sequence was amplified using the genomic DNA of Xanthomonas oryzae pv.…”

Section: Gene Cloning and Protein Expression And Purificationmentioning

confidence: 99%

“…Initial crystallization was performed as described in [10]. Crystals of XoGroEL were obtained on a submicroliter scale at 287 K by the sitting-drop vapor-diffusion method in 96-well Intelli plates (Hampton Research) using a Hydra II e-drop automated pipetting system (Matrix).…”

Section: Crystallization and X-ray Data Collectionmentioning

confidence: 99%

Crystal Structure of Chaperonin GroEL from Xanthomonas oryzae pv. oryzae

et al. 2019

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Xanthomonas oryzae pv. oryzae (Xoo) is a plant pathogen that causes bacterial blight of rice, with outbreaks occurring in most rice-growing countries. Thus far, there is no effective pesticide against bacterial blight. Chaperones in bacterial pathogens are important for the stabilization and delivery of effectors into host cells to cause disease. In bacteria, GroEL/GroES complex mediates protein folding and protects proteins against misfolding and aggregation caused by environmental stress. We determined the crystal structure of GroEL from Xanthomonas oryzae pv. oryzae (XoGroEL) at 3.2 Å resolution, which showed the open form of two conserved homoheptameric rings stacked back-to-back. In the open form structure, the apical domain of XoGroEL had a higher B factor than the intermediate and equatorial domains, indicating that the apical domain had a flexible conformation before the binding of substrate unfolded protein and ATP. The XoGroEL structure will be helpful in understanding the function and catalytic mechanism of bacterial chaperonin GroELs.

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Isolation, characterization and degradation performance of oxytetracycline degrading bacterium Planococcus sp. strain pw2

et al. 2022

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Crystallization and preliminary X-ray crystallographic analysis of the XoGroEL chaperonin fromXanthomonas oryzaepv.oryzae

Cited by 5 publications

References 27 publications

Oxytetracycline Degrading Potential of Lysinibacillus sp. Strain 3+I Isolated from Poultry Manure

Oxytetracycline Degrading Potential of Lysinibacillus sp. Strain 3+I Isolated from Poultry Manure

Crystal Structure of Chaperonin GroEL from Xanthomonas oryzae pv. oryzae

Isolation, characterization and degradation performance of oxytetracycline degrading bacterium Planococcus sp. strain pw2

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