Crystallization and preliminary X-ray crystallographic analysis of a thermostable organic solvent-tolerant lipase from<i>Bacillus</i>sp. strain 42

Khusaini, Mohd Saif; Rahman, Raja Noor Zaliha Raja Abd; Ali, Mohd Shukuri Mohamad; Leow, Adam Thean Chor; Basri, Mahiran; Salleh, Abu Bakar

doi:10.1107/s1744309111002028

Cited by 2 publications

(1 citation statement)

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“…Currently, only two thermostable lipases were reported grown by counter diffusion method, which are L42 lipase and L2 lipase. X-ray data of L42 lipase were collected at 2.0 Å and the crystal belonged to the monoclinic space group [ 15 ]. However thermostable L2 lipase crystal belonged to orthorhombic space group with resolution 2.7 Å [ 16 ].…”

Section: Resultsmentioning

confidence: 99%

Crystallographic Analysis of Ground and Space Thermostable T1 Lipase Crystal Obtained via Counter Diffusion Method Approach

Aris

Leow

Ali

et al. 2014

BioMed Research International

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Three-dimensional structure of thermostable lipase is much sought after nowadays as it is important for industrial application mainly found in the food, detergent, and pharmaceutical sectors. Crystallization utilizing the counter diffusion method in space was performed with the aim to obtain high resolution diffracting crystals with better internal order to improve the accuracy of the structure. Thermostable T1 lipase enzyme has been crystallized in laboratory on earth and also under microgravity condition aboard Progress spacecraft to the ISS in collaboration with JAXA (Japanese Aerospace Exploration Agency). This study is conducted with the aims of improving crystal packing and structure resolution. The diffraction data set for ground grown crystal was collected to 1.3 Å resolution and belonged to monoclinic C2 space group with unit cell parameters a = 117.40 Å, b = 80.95 Å, and c = 99.81 Å, whereas the diffraction data set for space grown crystal was collected to 1.1 Å resolution and belonged to monoclinic C2 space group with unit cell parameters a = 117.31 Å, b = 80.85 Å, and c = 99.81 Å. The major difference between the two crystal growth systems is the lack of convection and sedimentation in microgravity environment resulted in the growth of much higher quality crystals of T1 lipase.

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Section: Resultsmentioning

confidence: 99%