“…Assuming the presence of one subunit per asymmetric unit led to an empirically acceptable V M value of 2.79 Å 3 Da À1 , corresponding to a solvent content of 55.9% (Matthews, 1968). Since another S46 peptidase, bacterial DAP BII (Ogasawara et al, 1996;Sakamoto, Suzuki, Iizuka, Tateoka, Roppongi, Fujimoto et al, 2014;Sakamoto, Suzuki, Iizuka, Tateoka, Roppongi, Okada et al, 2014), was observed to be a dimer, we assume that PgDPP11 also forms a dimer. In that case, the molecular twofold axis of the dimeric PgDPP11 molecule must coincide with a crystallographic twofold axis of the C222 1 crystal of PgDPP11.…”