Crystallization and preliminary X-ray analysis of<i>Yersinia pseudotuberculosis</i>-derived mitogen

Donadini, Roberta; Wåhlberg, Malin; Kohsaka, Takao; Ito, Yasuhiko; Fields, Barry A.

doi:10.1107/s0907444903010710

Cited by 4 publications

(3 citation statements)

References 19 publications

(15 reference statements)

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“…they were not characterized further owing to suspected crystal twinning.' A further example is (13): 'The majority of crystals were twinned and it was difficult to identify single crystals solely based on appearance. Typically, only one crystal in ten was suitable for data collection, which severely hampered the search for heavy-atom derivatives.…”

Section: A Few Historical Examplesmentioning

confidence: 99%

Macromolecular crystal twinning, lattice disorders and multiple crystals1

Helliwell

2008

Crystallography Reviews

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The accuracy of the Content should not be relied upon and should be independently verified with primary sources of information. Taylor and Francis shall not be liable for any losses, actions, claims, proceedings, demands, costs, expenses, damages, and other liabilities whatsoever or howsoever caused arising directly or indirectly in connection with, in relation to or arising out of the use of the Content. This article may be used for research, teaching, and private study purposes. Any substantial or systematic reproduction, redistribution, reselling, loan, sub-licensing, systematic supply, or distribution in any form to anyone is expressly forbidden.

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Section: A Few Historical Examplesmentioning

confidence: 99%

Macromolecular crystal twinning, lattice disorders and multiple crystals1

Helliwell

2008

Crystallography Reviews

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“…The other SAg is Yersinia pseudotuberculosis mitogen (YPM), a virulence factor of Y. pseudotuberculosis 12 . The crystal structure of YPM is available, 13 but very little is known about its interaction with TCR and MHC class II molecule, because no clear results were obtained from directed mutation studies of YPM 14 …”

Section: Introductionmentioning

confidence: 99%

“…11 The other SAg is Yersinia pseudotuberculosis mitogen (YPM), a virulence factor of Y. pseudotuberculosis. 12 The crystal structure of YPM is available, 13 but very little is known about its interaction with TCR and MHC class II molecule, because no clear results were obtained from directed mutation studies of YPM. 14 Most SAgs have been isolated from the human pathogens S. aureus and S. pyogenes and are much more potent in humans than in mice, which to some extent reflects their better binding to human (human leucocyte antigen; HLA) than to mouse (H2) MHC class II molecules.…”

Section: Introductionmentioning

confidence: 99%

Superantigen‐presentation by rat major histocompatibility complex class II molecules RT1.B^l and RT1.D^l

et al. 2009

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SummaryRat major histocompatibility complex (MHC) class II molecules RT1.B l (DQ-like) and RT1.D l (DR-like) were cloned from the LEW strain using reverse transcription-polymerase chain reaction and expressed in mouse L929 cells. The transduced lines bound MHC class II-specific monoclonal antibodies in an MHC-isotype-specific manner and presented peptide antigens and superantigens to T-cell hybridomas. The T-cell-hybridomas responded well to all superantigens presented by human MHC class II, whereas the response varied considerably with rat MHC class II-transduced lines as presenters. The T-cell hybridomas responded to the pyrogenic superantigens Staphylococcus enterotoxin B (SEB), SEC1, SEC2 and SEC3 only at high concentrations with RT1.B l -transduced and RT1.D ltransduced cells as presenters. The same was true for streptococcal pyrogenic exotoxin A (SPEA), but this was presented only by RT1.B l and not by RT1.D l . SPEC was recognized only if presented by human MHC class II. Presentation of Yersinia pseudotuberculosis superantigen (YPM) showed no MHC isotype preference, while Mycoplasma arthritidis superantigen (MAS or MAM) was presented by RT1.D l but not by RT1.B l . Interestingly, and in contrast to RT1.B l , the RT1.D l completely failed to present SEA and toxic shock syndrome toxin 1 even after transduction of invariant chain (CD74) or expression in other cell types such as the surface MHC class II-negative mouse B-cell lymphoma (M12.4.1.C3). We discuss the idea that a lack of SEA presentation may not be a general feature of RT1.D molecules but could be a consequence of RT1.D l b-chain allelespecific substitutions (arginine 80 to lysine, asparagine 82 to aspartic acid) in the extremely conserved region flanking the Zn 2+ -binding histidine 81, which is crucial for high-affinity SEA-binding.

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Crystal and Solution Structures of a Superantigen from Yersinia pseudotuberculosis Reveal a Jelly-Roll Fold

Donadini¹,

Liew

Kwan

et al. 2004

Structure

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Superantigens are a class of microbial proteins with the ability to excessively activate T cells by binding to the T cell receptor. The staphylococcal and streptococcal superantigens are closely related in structure and possess an N-terminal domain that resembles an OB fold and a C-terminal domain similar to a beta-grasp fold. Yersinia pseudotuberculosis produces superantigens, YPMa, YPMb, and YPMc, which have no significant amino acid similarity to other proteins. We have determined the crystal and solution structures of YPMa, which show that the protein has a jelly-roll fold. The closest structural neighbors to YPMa are viral capsid proteins and members of the tumor necrosis factor superfamily. In the crystal structure, YPMa packs as a trimer, another feature shared with viral capsid proteins and TNF superfamily proteins. However, in solution YPMa behaves as a monomer, and any functional relevance of the trimer observed in the crystals is yet to be established.

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Crystallization and preliminary X-ray analysis ofYersinia pseudotuberculosis-derived mitogen

Cited by 4 publications

References 19 publications

Macromolecular crystal twinning, lattice disorders and multiple crystals1

Macromolecular crystal twinning, lattice disorders and multiple crystals1

Superantigen‐presentation by rat major histocompatibility complex class II molecules RT1.B^l and RT1.D^l

Crystal and Solution Structures of a Superantigen from Yersinia pseudotuberculosis Reveal a Jelly-Roll Fold

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Crystallization and preliminary X-ray analysis ofYersinia pseudotuberculosis-derived mitogen

Cited by 4 publications

References 19 publications

Macromolecular crystal twinning, lattice disorders and multiple crystals1

Macromolecular crystal twinning, lattice disorders and multiple crystals1

Superantigen‐presentation by rat major histocompatibility complex class II molecules RT1.Bl and RT1.Dl

Crystal and Solution Structures of a Superantigen from Yersinia pseudotuberculosis Reveal a Jelly-Roll Fold

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Superantigen‐presentation by rat major histocompatibility complex class II molecules RT1.B^l and RT1.D^l