Crystallization and preliminary crystallographic analysis of the central domain ofDrosophilaDribble, a protein that is essential for ribosome biogenesis

Abstract

Dribble (DBE) is a Drosophila protein that is essential for ribosome biogenesis. Bioinformatics analysis revealed a folded central domain of DBE which is flanked by structural disorder in the N-and C-terminal regions. The protein fragment spanning amino-acid residues ) was produced for structural determination. In this report, the crystallization and preliminary X-ray diffraction data analysis of the DBE protein domain are described. Crystals of DBE were grown by the sitting-drop vapour-diffusion method at 289 K using ammonium phosphate as a precipitant. The crystals belonged to space group P2 1 2 1 2 1 . Data were collected that extended to beyond 2 Å resolution.