2014
DOI: 10.1021/bi500244m
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Crystal Structures of the F and pSLT Plasmid TraJ N-Terminal Regions Reveal Similar Homodimeric PAS Folds with Functional Interchangeability

Abstract: In the F family of conjugative plasmids, TraJ is an essential transcriptional activator of the tra operon that encodes most of the proteins required for conjugation. Here we report for the first time the X-ray crystal structures of the TraJ N-terminal domains from the prototypic F plasmid (TraJF11–130) and from the Salmonella virulence plasmid pSLT (TraJpSLT1–128). Both structures contain similar Per-ARNT-Sim (PAS) folds, which further homodimerize through the N-terminal helix and the structurally conserved β-… Show more

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Cited by 7 publications
(15 citation statements)
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References 56 publications
(128 reference statements)
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“…However, in TraJ V the HTH domain is located in the N-terminal half of the protein. A comparison between the predicted 3D structure for TraJ V N-terminal domain and the solved structure for F plasmid TraJ N-terminal domain (pdb 4KQD) (Lu et al, 2014) showed no structural homology (Figure 5C). In fact, TraJ is predicted to be similar to the canonical LuxR protein, with an N-terminal effector binding domain and a C-terminal DBD.…”
Section: Resultsmentioning
confidence: 99%
“…However, in TraJ V the HTH domain is located in the N-terminal half of the protein. A comparison between the predicted 3D structure for TraJ V N-terminal domain and the solved structure for F plasmid TraJ N-terminal domain (pdb 4KQD) (Lu et al, 2014) showed no structural homology (Figure 5C). In fact, TraJ is predicted to be similar to the canonical LuxR protein, with an N-terminal effector binding domain and a C-terminal DBD.…”
Section: Resultsmentioning
confidence: 99%
“…The putative TraJ-binding inverted repeats in the F, R1, and R100 plasmids share little sequence homology, consistent with the previous finding that TraJ exclusively acts on its cognate P Y among these three plasmids. In contrast, the putative TraJ-binding repeats in R1 and pSLT plasmids are quite similar, explaining the cross-activity of TraJ between these two F-like plasmids (11,16).…”
Section: Figmentioning
confidence: 97%
“…Activation of P Y requires TraJ, a transcriptional activator encoded by the gene immediately upstream of the tra operon (14), and the activity of P Y is a function of the TraJ level (8). TraJ has an N-terminal PAS (Per-Arnt-Sim) domain responsible for dimerization and a C-terminal helix-turn-helix (HTH) motif that carries allelic specificity in recognizing its cognate plasmid DNA (11,(15)(16)(17). A previous DNase I footprinting experiment indicated that TraJ specifically binds to an inverted repeat sequence immediately upstream of the Ϫ35 region of P Y in the F-like plasmid R100 (18).…”
mentioning
confidence: 99%
“…TraJ contains a second domain that represents the DNA-binding part of the protein as suggested by the presence of a helix-turn-helix DNA-binding motif. It was also shown that this domain, located in the C-terminal half of TraJ, confers DNA-binding specificity (134). Both the PAS domain and the DNA-binding domain are essential for the function of TraJ (135), which also served to define subgroups within the group of MOB F12 A plasmids (see Table 1).…”
Section: Regulation Of Dna Transfer Genes: On and Off Switchesmentioning
confidence: 99%
“…Both the PAS domain and the DNA-binding domain are essential for the function of TraJ (135), which also served to define subgroups within the group of MOB F12 A plasmids (see Table 1). Importantly, the sequence differences between TraJ proteins result in plasmid subgroup-specific activation of the P Y promoter which differs in the proposed sequence (centered at −44 relative to the transcription start site) that is likely contacted by TraJ (15,128,134). Therefore, in situations where two compatible F-like plasmids reside in the same cell, TraJ escape from FinOP in one plasmid would result in transfer gene activation and subsequent DNA transfer of only one of the two plasmids.…”
Section: Regulation Of Dna Transfer Genes: On and Off Switchesmentioning
confidence: 99%