Crystal structures ofClostridium pasteurianumrubredoxin mutants V8A and V8N

Abstract

vapor diffusion method at 20•C. The crystals of azmin-I belong to the monoclinic crystal system and have space group C2 with unit cell parameters of a=l30.6A, b=54.4A, c=74.7 A, and b=96.l•. Four molecules are in the asymmetric unit. The crystals of azmin-II belong to the tetragonal crystal system and have space group ?4122 ,,;th unit cell p';rameters a=b=52.6A, c=100.7 A. Only one molecule is in the asymmet1ic unit. They diffract up to 2.8A and 2.0A resolutions, respectively. Their crystal structures were solved by molecular replacement method using AMoRe in the CCP4. Molecular structure of Azurin ll from Alcaligenes Xylosoxidans NCIB 11015 was chosen as a starting model for both azurins. The refinement for both azmins is being carried out by XPLOR.