Crystal structure of the protein At3g01520, a eukaryotic universal stress protein‐like protein from arabidopsis thaliana in complex with AMP

Abstract

Members of the universal stress protein (USP) family are conserved in a phylogenetically diverse range of prokaryotes, fungi, protists, and plants and confer abilities to respond to a wide range of environmental stresses. Arabidopsis thaliana contains 44 USP domain-containing proteins, and USP domain is found either in a small protein with unknown physiological function or in an N-terminal portion of a multi-domain protein, usually a protein kinase. Here, we report the first crystal structure of a eukaryotic USP-like protein encoded from the gene At3g01520. The crystal structure of the protein At3g01520 was determined by the single-wavelength anomalous dispersion method and refined to an R factor of 21.8% (Rfree = 26.1%) at 2.5 Å resolution. The crystal structure includes three At3g01520 protein dimers with one AMP molecule bound to each protomer, comprising a Rossmann-like α/β overall fold. The bound AMP and conservation of residues in the ATP-binding loop suggest that the protein At3g01520 also belongs to the ATP-binding USP subfamily members.

Virus-induced gene silencing of the RPC5-like subunit of RNA polymerase III caused pleiotropic effects in Nicotiana benthamiana

“…The up-regulated MPL43 protein participates in abscisic acid (ABA) signal transduction, which is critical for plant growth and responses to abiotic stress. The universal stress protein (USP), induced nearly 5-fold in VIGS plants, mediates cellular responses to a wide variety of stresses30. Overall, a significant number of DEGs related to stress responses in VIGS plants may indicate that silencing of the RPC5L affected not only core functions of the RNAPIII that is, production of small housekeeping RNAs, but also had a broader influence on other processes, including defense responses.…”

Exploring Machine Learning Algorithms to Unveil Genomic Regions Associated With Resistance to Southern Root-Knot Nematode in Soybeans

“…Universal Stress Proteins (USP) are involved in multiple cellular responses to biotic and abiotic stressors, ranging from ion scavenging, hypoxia responses, cellular mobility, and regulation of cell growth and development ( Chi et al, 2019 ). Glyma.10g013700 has been associated with the Arabidopsis thaliana AT3G01520 , an adenine nucleotide alpha hydrolases-like superfamily protein that is involved in N-terminal protein myristoylation ( Kim et al, 2015 ). The attachment of a myristoyl group enhances specific protein–protein interactions, thus playing an essential role in membrane targeting and signal transduction in plant responses to biotic and abiotic stressors ( Podell and Gribskov, 2004 ; Traverso et al, 2008 ; Udenwobele et al, 2017 ).…”

Physiological and transcriptomic analyses characterized high temperature stress response mechanisms in Sorbus pohuashanensis

“…Then, cAMP-activated protein kinase A (PKA). PKA regulates the activities of various proteins, including TFs, through phosphorylation, so as to regulate a variety of physiological molecules in cells such as ion channels, cytoskeleton www.nature.com/scientificreports/ proteins, and enzymes 42 . PLDδ may regulate signal transduction in the process of plant stress resistance through phosphatidic acid (PA), an important signaling molecule in plant cell signal transduction 43 .…”

“…Besides, some resistance proteins e.g. resistance gene analogs (RGAs), vicilin and universal stress proteins(USPs) seem to play a role in maintaining protein homeostasis of S. pohuashanensis under high-temperature stress 42,81 .…”