Crystal structure of the human calpain-5 catalytic core

Abstract

Calpain-5 (CAPN5) is a calcium-activated cysteine protease that is expressed throughout the central nervous system and retina. Mutations in the catalytic core of CAPN5 make it hyperactive and cause autosomal dominant neovascular inflammatory vitreoretinopathy (ADNIV; OMIM#193235), a severe, blinding eye disease. Although crystal structures have been solved for some classical calpains (e.g. CAPN1 and 2),