Crystal structure of intracellular family 1 β‐glucosidase BGL1A from the basidiomycete Phanerochaete chrysosporium

Abstract

The white-rot fungus Phanerochaete chrysosporium has two intracellular b-glucosidases (BGL1A and BGL1B) belonging to glycoside hydrolase (GH) family 1. BGL1B effectively hydrolyzes cellobiose and cellobionolactone, but BGL1A does not. We have determined the crystal structure of BGL1A in substrate-free and gluconolactone complexed forms. The overall structure and the characteristic of subsite À1 (glycone site) were similar to those of other known GH1 enzymes. The loop regions covering on the (b/a) 8 barrel was significantly deviated, and they form a unique subsite +1 (aglycone site) of BGL1A.

Differential Involvement of β-Glucosidases from Hypocrea jecorina in Rapid Induction of Cellulase Genes by Cellulose and Cellobiose

“…S2) (11). Specifically, while the surrounding residues at the glycone binding site are highly conserved, residues around the aglycone binding site, which have been proposed to be the basis of substrate specificity (24), are highly divergent. Residues with smaller side chains at the aglycone site have also been observed in BGL1B from P. chrysosporium, which is more efficient than BGL1A in hydrolysis of cellobiose (32).…”

Microbial β-Glucosidases: Screening, Characterization, Cloning and Applications

“…Similarly, β-glucosidases belonging to GH family 3 contain 600-900 amino acids in length with molecular weight of 65-90 kDa per subunit, but because these group of enzymes are usually glycosylated, their molecular mass ranges 110-130 kDa [44,157,158]. β-Glucosidase varies in quaternary structure arrangement, for example, monomeric [159], dimeric [160], trimeric [147], tetrameric [151] enzymes have been reported. Native molecular weight therefore may be higher than identified by SDS-PAGE [161].…”

Structural and Enzymatic Characterization of Os3BGlu6, a Rice β-Glucosidase Hydrolyzing Hydrophobic Glycosides and (1→3)- and (1→2)-Linked Disaccharides

“…Simplified phylogenetic tree of the amino acid sequences of eukaryotic GH1 proteins with known structures and those of rice and Arabidopsis GH1 gene products. The protein sequences of the eukaryotic proteins with known structures are marked with four-character PDB codes for one of their structures, including Trifolium repens cyanogenic b-glucosidase (1CBG; Barrett et al, 1995), Sinapsis alba myrosinase (1MYR; Burmeister et al, 1997), Zea mays ZmGlu1 b-glucosidase (1E1F; Czjzek et al, 2000), Sorghum bicolor Dhr1 dhurrinase (1V02; Verdoucq et al, 2004), Triticum aestivum b-glucosidase (2DGA; Sue et al, 2006), Rauvolfia serpentina strictosidine b-glucosidase (2JF6; Barleben et al, 2007), and Oryza sativa Os3BGlu7 (BGlu1) b-glucosidase (2RGL; Chuenchor et al, 2008) from plants, along with Brevicoryne brassicae myrosinase (1WCG; Husebye et al, 2005), Homo sapiens cytoplasmic (Klotho) b-glucosidase (2E9M; Hayashi et al, 2007), and Phanerochaete chrysosporium (2E3Z; Nijikken et al, 2007), while those encoded in the Arabidopsis and rice genomes are labeled with the systematic names given by Xu et al (2004) and Opassiri et al (2006), respectively. One or two example proteins from each plant are given for each of the eight clusters of genes shared by Arabidopsis (At) and rice (Os) and the Arabidopsis-specific clusters At I (b-glucosidases) and At II (myrosinases), with the number of Arabidopsis or rice enzymes in each cluster given in parentheses.…”