Crystal Structure of Grape Dihydroflavonol 4-Reductase, a Key Enzyme in Flavonoid Biosynthesis

Petit, P.; Granier, T.; d’Estaintot, Béatrice Langlois; Manigand, Claude; Bathany, Katell; Schmitter, Jean‐Marie; Lauvergeat, Virginie; Hamdi, Saı̈d; Gallois, B.

doi:10.1016/j.jmb.2007.02.088

Cited by 140 publications

(151 citation statements)

References 51 publications

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“…The DFRs (AB201759, AF029685, AY575027, AY695812, and AY695813) of Fragaria x ananassa, which accumulate pelargonidin, contain alanine in this position. These results agree with the view that residue 133 alone does not determine substrate specificity, as reported previously (Petit et al 2007).…”

supporting

confidence: 93%

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Generation of pink flower varieties from blue Torenia hybrida by redirecting the flavonoid biosynthetic pathway from delphinidin to pelargonidin

Nakamura

Fukuchi-Mizutani

Fukui

et al. 2010

Plant Biotechnology

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supporting

confidence: 93%

“…However, substituting Asp at position 133 for Asn in the grape DFR does not change the substrate specificity (Petit et al 2007). The pelargonium DFR isolated in this study has Asp in this position and catalyzes DHK.…”

mentioning

confidence: 99%

Generation of pink flower varieties from blue Torenia hybrida by redirecting the flavonoid biosynthetic pathway from delphinidin to pelargonidin

Nakamura

Fukuchi-Mizutani

Fukui

et al. 2010

Plant Biotechnology

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“…The activity of the wild-type enzyme and its variant N133D has been quantified in vitro, using dihydroquercetin or dihydrokaempferol. Results demonstrate that position 133 cannot be solely responsible for the recognition of the B-ring hydroxylation pattern of dihydroflavonols" [30]. This work may aid in future experiments for biomanufacture of anthocyanins and tannins via metabolic engineering [2,30].…”

Section: Understanding Biosynthesis Of Anthocyanins and Condensed Tanmentioning

confidence: 99%

“…Dihydroflavonol reductase (DHF) PDB code 2C29: "The nicotinamide adenine dinucleotide phosphate (NADPH)-dependent enzyme dihydroflavonol 4-reductase (DFR) catalyzes a late step in the biosynthesis of anthocyanins and condensed tannins, two flavonoid classes of importance to plant survival and human nutrition" (30). The crystal structure of Vitis vinifera DFR, was determined for detailed structure-function studies.…”

Section: Understanding Biosynthesis Of Anthocyanins and Condensed Tanmentioning

confidence: 99%

Structural Analysis of Flavonoid/Drug Target Complexes: Natural Products as Lead Compounds for Drug Development

Sosa¹,

Sosa²,

Phansalkar³

et al. 2017

Nat Prod Chem Res

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Plants produce pigments called flavonoids that are synthesized (de novo) primarily from the amino acid phenylalanine. Many natural products such as herb preparations for medicinal use contain flavonoids and so these diverse structural compounds have become potential lead compounds for a variety of illnesses ranging from specific cancers to gout. Recently, Biotechnology has been utilized through bioorganic engineering to manufacture flavonoid and modified flavonoids for medicinal applications. A growing number of protein-flavonoid complexes have been crystallized and their structures solved in the last decade. A summary of the protein-flavonoid complexes with medicinal relevance is presented herein. A detailed analysis of selected protein-flavonoid complexes is provided. The goal is to provide insights for modifications to known flavonoids that could be biomanufactured to generate more specific and efficient binding of flavonoids to protein targets with medicinal relevance.

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“…The zone type of restriction fragment length polymerphism (RFLP) indicated that DFR was a single copy gene in both potato and tomato (De et al, 2003;Bongue-Bartelsman et al, 1994). In grape, crystal structure of DFR protein is a complex with three subunits and has a specific NADPH domain (Petit and Granier, 2007).…”

Section: Introductionmentioning

confidence: 99%

Cloning and Bioinformatical Analysis of Dihydroflavonol 4-reductase Gene from Pigmented Potato

Xiao¹,

Wang²,

Li³

et al. 2012

pgt

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The main pigment which affects flower colors is the anthocyanin, and dihydroflavonol 4-reductase (DFR) is a key enzyme in anthocyanin synthesis. In this study, we employed the native pigmented potato varieties 'Jianchuanhong' and 'Zhuanxinwu' from Yunnan Province, and the introduced potato variety 'Heimeiren' as the experimental materials. The complete cDNAs sequence of DFR was cloned from their tubers epidermis by RT-PCR, and the bioinformatics analysis was carried out. The sequence analysis displayed that DFR gene of the three tested pigmented potato varieties encoded 381 amino acid and had a complete open reading frame (ORF). It also showed that DFR had a highly conserved NADP(H)-binding site and substrate specificity site. Homologous alignment result demonstrated that the nucleotide similarity and amino acid homology of the three kinds of pigmente potato species are 99.24%, 98.78%, respectively. And the amino acid homology of these pigmented potatoes with other Solanaceae plants, such as Nicotiana alata and Petunia x hybrida, were more than 86%. None DFR of these pigmente potatoes had signal peptide, obvious transmembrane structure region, we speculated that DFR might be a kind of hydrophilic protein which probably located in cytoplasm. We still found that α-helix and random coil were primary secondary structural components of DFR which belong to NADB-Rossmann superfamily.

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Crystal Structure of Grape Dihydroflavonol 4-Reductase, a Key Enzyme in Flavonoid Biosynthesis

Cited by 140 publications

References 51 publications

Generation of pink flower varieties from blue Torenia hybrida by redirecting the flavonoid biosynthetic pathway from delphinidin to pelargonidin

Generation of pink flower varieties from blue Torenia hybrida by redirecting the flavonoid biosynthetic pathway from delphinidin to pelargonidin

Structural Analysis of Flavonoid/Drug Target Complexes: Natural Products as Lead Compounds for Drug Development

Cloning and Bioinformatical Analysis of Dihydroflavonol 4-reductase Gene from Pigmented Potato

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