Crystal structure of chloroplastic thioredoxin z defines a type‐specific target recognition

Moigne, Théo Le; Gurrieri, Libero; Crozet, Pierre; Marchand, Christophe; Zaffagnini, Mirko; Sparla, Francesca; Lemaire, Stéphane D.; Henri, Julien

doi:10.1111/tpj.15300

Cited by 8 publications

(3 citation statements)

References 114 publications

(240 reference statements)

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“…4B , Setup 2) ( Petroutsos et al 2011 ; Wang et al 2016 ). Thioredoxin z (TRXz) has been shown to activate Calvin-Benson cycle protein phosphoribulokinase in vitro ( Le Moigne et al 2021 ). Plant TRXy interacts with 2-Cys peroxiredoxins ( Jurado-Flores et al 2020 ).…”

Section: Resultsmentioning

confidence: 99%

TurboID reveals the proxiomes of Chlamydomonas proteins involved in thylakoid biogenesis and stress response

Kreis,

König,

Misir

et al. 2023

Plant Physiology

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In Chlamydomonas (Chlamydomonas reinhardtii) the VESICLE-INDUCING PROTEINS IN PLASTIDS 1 and 2 (VIPP1 and VIPP2) play roles in the sensing and coping with membrane stress and in thylakoid membrane biogenesis. To gain more insight into these processes, we aimed to identify proteins interacting with VIPP1/2 in the chloroplast and chose proximity labeling (PL) for this purpose. We used the transient interaction between the nucleotide exchange factor CHLOROPLAST GRPE HOMOLOG 1 (CGE1) and the stromal HEAT SHOCK PROTEIN 70B (HSP70B) as test system. While PL with APEX2 and BioID proved to be inefficient, TurboID resulted in substantial biotinylation in vivo. TurboID-mediated PL with VIPP1/2 as baits under ambient and H2O2 stress conditions confirmed known interactions of VIPP1 with VIPP2, HSP70B, and the CHLOROPLAST DNAJ HOMOLOG 2 (CDJ2). Proteins identified in the VIPP1/2 proxiome can be grouped into proteins involved in the biogenesis of thylakoid membrane complexes and the regulation of photosynthetic electron transport, including PROTON GRADIENT REGULATION 5-LIKE 1 (PGRL1). A third group comprises 11 proteins of unknown function whose genes are upregulated under chloroplast stress conditions. We named them VIPP PROXIMITY LABELING (VPL1-11). In reciprocal experiments, we confirmed VIPP1 in the proxiomes of VPL2 and PGRL1. Our results demonstrate the robustness of TurboID-mediated PL for studying protein interaction networks in the chloroplast of Chlamydomonas and pave the way for analyzing functions of VIPPs in thylakoid biogenesis and stress responses.

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Section: Resultsmentioning

confidence: 99%

TurboID reveals the proxiomes of Chlamydomonas proteins involved in thylakoid biogenesis and stress response

Kreis,

König,

Misir

et al. 2023

Plant Physiology

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“…CAS also links a Ca 2+ signal to the high light-induced expression of the LHCSR3 gene that is crucial for nonphotochemical quenching (Petroutsos et al, 2011). Thioredoxin z (TRXz) has been shown to activate Calvin-Benson cycle protein phosphoribulokinase in vitro (Le Moigne et al, 2021). Plant TRXy interacts with 2-Cys peroxiredoxins but the physiological relevance of this interaction is not clear (Jurado-Flores et al, 2020).…”

Section: Resultsmentioning

confidence: 99%

TurboID reveals the proxiomes of CGE1, VIPP1, and VIPP2 inChlamydomonas reinhardtii

Kreis¹,

König²,

Sommer³

et al. 2022

Preprint

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In Chlamydomonas reinhardtii, VIPP1 and VIPP2 play a role in the sensing and coping with membrane stress and in thylakoid membrane biogenesis. To gain more insight into these processes, we aimed to identify proteins interacting with VIPP1/2 in the chloroplast and chose proximity labeling (PL) for this purpose. We used the transient interaction between the nucleotide exchange factor CGE1 and stromal HSP70B as test system. While PL with APEX2 and BioID proved to be inefficient, TurboID resulted in significant biotinylation in vivo. TurboID-mediated PL with VIPP1/2 as baits under ambient and H2O2 stress conditions confirmed known interactions of VIPP1 with VIPP2, HSP70B and CDJ2. Novel proteins in the VIPP1/2 interaction network can be grouped into proteins involved in the biogenesis of thylakoid membrane complexes and the regulation of photosynthetic electron transport. A third group comprises 11 proteins of unknown function whose genes are upregulated under chloroplast stress conditions. We named them VIPP PROXIMITY LABELING (VPL1-11) and confirmed the proximity of VIPP1 and VPL2 in a reciprocal experiment. Our results demonstrate the robustness of TurboID-mediated PL for studying protein interaction networks in the chloroplast of Chlamydomonas and pave the way for analyzing functions of VIPPs in thylakoid biogenesis and stress responses.

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“…2005 , Yoshida et al. 2015 , Le Moigne et al. 2021 ), which confer different target-recognition mechanisms on each Trx subtype.…”

Section: Functional Specificity and Redundancy Of Trx Subtypesmentioning

confidence: 99%

Current Insights into the Redox Regulation Network in Plant Chloroplasts

Yoshida

Hisabori

2023

Plant and Cell Physiology

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Thiol/disulfide-based redox regulation is a ubiquitous post-translational protein modification. In plant chloroplasts, this regulatory mechanism is tightly associated with the light-dependent activation of photosynthetic enzymes (e.g., Calvin–Benson cycle enzymes). A thioredoxin (Trx)-mediated pathway was discovered to transmit light signals as a reducing power about half a century ago; since then, it has been accepted as the basic machinery of chloroplast redox regulation. However, during the past two decades, it has been increasingly apparent that plants have acquired multiple Trx isoforms and Trx-like proteins in chloroplasts. Furthermore, proteomics-based analyses have identified various chloroplast enzymes as potential targets of redox regulation. These facts highlight the necessity to revisit the molecular basis and physiological importance of the redox regulation system in chloroplasts. Recent studies have revealed novel aspects of this system, including unprecedented redox-regulated processes in chloroplasts and the functional diversity of Trx family proteins. Of particular significance is the identification of protein-oxidizing pathways that turn off photosynthetic metabolism during light-to-dark transitions. In this review, we summarize current insights into the redox regulation network in chloroplasts.

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Crystal structure of chloroplastic thioredoxin z defines a type‐specific target recognition

Cited by 8 publications

References 114 publications

TurboID reveals the proxiomes of Chlamydomonas proteins involved in thylakoid biogenesis and stress response

TurboID reveals the proxiomes of Chlamydomonas proteins involved in thylakoid biogenesis and stress response

TurboID reveals the proxiomes of CGE1, VIPP1, and VIPP2 inChlamydomonas reinhardtii

Current Insights into the Redox Regulation Network in Plant Chloroplasts

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