Crystal structure of a novel putative sugar isomerase from the psychrophilic bacterium Paenibacillus sp. R4

Kwon, Sunghoon; Ha, Hyekyung; Kang, Yong Jun; Sung, Ji Hye; Hwang, Jisub; Lee, Min Ju; Lee, Jun Hyuck; Park, Hyun Ho

doi:10.1016/j.bbrc.2021.11.026

Cited by 2 publications

(1 citation statement)

References 31 publications

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“…These distinct conformations of the C-terminal region of TcaXI and TthXI caused different structural properties for the tetrameric assembly. These different conformations of the C-terminal hook-shaped region have been suggested to affect the tight tetrameric structure [47].…”

Section: Structural Comparison Of Savxi With Other XI Structuresmentioning

confidence: 99%

Structural Analysis of Xylose Isomerase from Streptomyces avermitilis

Nam

2024

Crystals

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Xylose isomerase (XI, also known as glucose isomerase) is an oxidoreductase that interconverts aldoses and ketoses. XI catalyzes the reversible isomerization of D-glucose and D-xylose into D-fructose and D-xylulose, respectively. The molecular function of XI is widely applied in producing high-fructose corn syrup (HFCS) in the food industry and bioethanol from hemicellulose in the biofuel industry. The structural information of XI from diverse strains is important for understanding molecular properties that can provide insights into protein engineering to improve enzyme efficiency. To extend the knowledge of the structural information on XI, the crystal structure of XI from Streptomyces avermitilis (SavXI) was determined at a 2.81 Å resolution. SavXI containing TIM barrel and extended α-helix domains formed the tetrameric assembly. The two metal-binding sites and their coordinating residues showed diverse conformations, providing the structural flexibility of the active site of SavXI. The structural comparison of SavXI and XI homologs exhibited unique metal-binding sites and conformations of the C-terminal α-helix domain. These structural results extend our knowledge of the molecular flexibility and mechanism of the XI family.

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Section: Structural Comparison Of Savxi With Other XI Structuresmentioning

confidence: 99%