Crystal‐Structure‐Guided Design of Self‐Assembling RNA Nanotriangles

Boerneke, Mark A.; Dibrov, Sergey M.; Hermann, Thomas

doi:10.1002/anie.201600233

Cited by 39 publications

(37 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Liposomes are known to accumulate in the liver, and the drug-loaded liposomes can cause significant liver toxicity 42 . Nucleic acids, beyond the native function for RNA interference 43–45 , enzyme-like activity 46 , DNA repair 47 , and other genome editing 48 , can also be designed and constructed with defined shape and structure 49,50 . Nucleic acid nanoparticles have great potential to overcome the liver accumulation limitations for in vivo application 23,24,27,28,31,51 , with the nature of negative surface charge and well defined particle shape and size.…”

Section: Discussionmentioning

confidence: 99%

RNA nanoparticles harboring annexin A2 aptamer can target ovarian cancer for tumor-specific doxorubicin delivery

Zhang

et al. 2017

Nanomedicine: Nanotechnology, Biology and Medicine

View full text Add to dashboard Cite

A novel modified nucleic acid nanoparticle harboring an annexin A2 aptamer for ovarian cancer cell targeting and a GC rich sequence for doxorubicin loading is designed and constructed. The system utilizes a highly stable three-way junction (3WJ) motif from phi29 packaging RNA as a core structure. A phosphorothioate-modified DNA aptamer targeting annexin A2, Endo28, was conjugated to one arm of the 3WJ. The pRNA-3WJ motif retains correct folding of attached aptamer, keeping its functions intact. It is of significant utility for aptamer-mediated targeted delivery. The DNA/RNA hybrid nanoparticles remained intact after systemic injection in mice and strongly bound to tumors with little accumulation in healthy organs 6 hours post-injection. The Endo28-3WJ-Sph1/Dox intercalates selectively enhanced toxicity to annexin A2 positive ovarian cancer cells in vitro. The constructed RNA/DNA hybrid nanoparticles can potentially enhance the therapeutic efficiency of doxorubicin at low doses for ovarian cancer treatment through annexin A2 targeted drug delivery.

show abstract

Section: Discussionmentioning

confidence: 99%

RNA nanoparticles harboring annexin A2 aptamer can target ovarian cancer for tumor-specific doxorubicin delivery

Zhang

et al. 2017

Nanomedicine: Nanotechnology, Biology and Medicine

View full text Add to dashboard Cite

show abstract

“…Copyright 2013 RNA society); (b) RNA square with tectoRNA motif (Reprinted with permission from Chworos et al (2004). Copyright 2004 The American Association for the Advancement of Science); (c) RNA triangle with IIa motif from SVV IRES (Reprinted with permission from Boerneke et al (2016) Dibrov et al (2011). Copyright 2011 National Academy of Sciences; (d) RNA triangle and square with k-turn motif (Reprinted with permission from Huang and Lilley (2016).…”

Section: Advantages Of Rna Nanotechnology For Cancer Targeting and mentioning

confidence: 99%

“…As a result of the highly influential research in previous traditional RNA biology, the field of RNA nanotechnology has experienced an exponential growth in the past decade (Jasinski et al, ; Li et al, ). Myriads of sophisticated RNA architectures with highly‐ordered structures and multivalent functionalities were assembled using RNA as building blocks, such as RNA polygons (Boerneke, Dibrov, & Hermann, ; Bui et al, ; Dibrov, McLean, Parsons, & Hermann, ; Huang & Lilley, ; Jasinski, Khisamutdinov, Lyubchenko, & Guo, ; Khisamutdinov et al, ; Ohno et al, ; Severcan, Geary, Verzemnieks, Chworos, & Jaeger, ), RNA polyhedrons (Afonin et al, ; Hao et al, ; Khisamutdinov et al, ; Li et al, ; Severcan et al, ; Xu et al, ; Yu, Liu, Jiang, Wang, & Mao, ), RNA rings (Geary, Chworos, Verzemnieks, Voss, & Jaeger, ; Grabow et al, ; Shu et al, ), RNA dendrimers (Sharma et al, ), jigsaw puzzles (Chworos et al, ), and RNA filaments (Nasalean, Baudrey, Leontis, & Jaeger, ) (Figure ).…”

Section: Introductionmentioning

confidence: 99%

Tuning the size, shape and structure of RNA nanoparticles for favorable cancer targeting and immunostimulation

Guo

Yin

et al. 2019

WIREs Nanomed Nanobiotechnol

View full text Add to dashboard Cite

The past decade has shown exponential growth in the field of RNA nanotechnology. The rapid advances of using RNA nanoparticles for biomedical applications, especially targeted cancer therapy, suggest its potential as a new generation of drug. After the first milestone of small molecule drugs and the second milestone of antibody drugs, it was predicted that RNA drugs, either RNA itself or chemicals/ligands that target RNA, will be the third milestone in drug development. Thus, a comprehensive assessment of the current therapeutic RNA nanoparticles is urgently needed to meet the drug evaluation criteria. Specifically, the pharmacological and immunological profiles of RNA nanoparticles need to be systematically studied to provide insights in rational design of RNA‐based therapeutics. By virtue of its programmability and biocompatibility, RNA molecules can be designed to construct sophisticated nanoparticles with versatile functions/applications and highly tunable physicochemical properties. This intrinsic characteristic allows the systemic study of the effects of various properties of RNA nanoparticles on their in vivo behaviors such as cancer targeting and immune responses. This review will focus on the recent progress of RNA nanoparticles in cancer targeting, and summarize the effects of common physicochemical properties such as size and shape on the RNA nanoparticles' biodistribution and immunostimulation profiles. This article is categorized under: Biology‐Inspired Nanomaterials > Nucleic Acid‐Based Structures Diagnostic Tools > in vivo Nanodiagnostics and Imaging Therapeutic Approaches and Drug Discovery > Nanomedicine for Oncologic Disease

show abstract

“…The last decade has seen significant advances in the design, characterization, and application of DNA crystals. It is now routine to produce robust DNA crystals from specific motifs, and there continue to be advances in RNA crystal design [85]. Both the conventional Watson-Crick paired tensegrity triangle and the novel parallel GGA-GGA motif can be used to produce crystalline arrangements, and new designs and motifs will further expand the exploration of DNA crystals.…”

Section: Future Directions and Applicationsmentioning

confidence: 99%

3D DNA Crystals and Nanotechnology

Paukstelis

Seeman

2016

Crystals

View full text Add to dashboard Cite

DNA's molecular recognition properties have made it one of the most widely used biomacromolecular construction materials. The programmed assembly of DNA oligonucleotides has been used to create complex 2D and 3D self-assembled architectures and to guide the assembly of other molecules. The origins of DNA nanotechnology are rooted in the goal of assembling DNA molecules into designed periodic arrays, i.e., crystals. Here, we highlight several DNA crystal structures, the progress made in designing DNA crystals, and look at the current prospects and future directions of DNA crystals in nanotechnology.

show abstract

Crystal‐Structure‐Guided Design of Self‐Assembling RNA Nanotriangles

Cited by 39 publications

References 22 publications

RNA nanoparticles harboring annexin A2 aptamer can target ovarian cancer for tumor-specific doxorubicin delivery

RNA nanoparticles harboring annexin A2 aptamer can target ovarian cancer for tumor-specific doxorubicin delivery

Tuning the size, shape and structure of RNA nanoparticles for favorable cancer targeting and immunostimulation

3D DNA Crystals and Nanotechnology

Contact Info

Product

Resources

About