Crystal structure and RNA-binding analysis of the archaeal transcription factor NusA

Shibata, Rie; Bessho, Yoshitaka; Shinkai, Akeo; Nishimoto, Madoka; Fusatomi, Emiko; Terada, Takaho; Shirouzu, Mikako; Yokoyama, Shigeyuki

doi:10.1016/j.bbrc.2007.01.119

Cited by 17 publications

(17 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The transcriptional regulation-related proteins corresponded to a putative transcription regulator (spot 32) and the transcription factor NusA (spot 33) involved in the intrinsic termination of transcription [57]. NusA has been also reported to display important functions in the cellular response to adverse factors [58].…”

Section: Resultsmentioning

confidence: 99%

Molecular Characterization of Copper and Cadmium Resistance Determinants in the Biomining Thermoacidophilic ArchaeonSulfolobus metallicus

Orell

Remonsellez

Arancibia

et al. 2013

Archaea

View full text Add to dashboard Cite

Sulfolobus metallicus is a thermoacidophilic crenarchaeon used in high-temperature bioleaching processes that is able to grow under stressing conditions such as high concentrations of heavy metals. Nevertheless, the genetic and biochemical mechanisms responsible for heavy metal resistance in S. metallicus remain uncharacterized. Proteomic analysis of S. metallicus cells exposed to 100 mM Cu revealed that 18 out of 30 upregulated proteins are related to the production and conversion of energy, amino acids biosynthesis, and stress responses. Ten of these last proteins were also up-regulated in S. metallicus treated in the presence of 1 mM Cd suggesting that at least in part, a common general response to these two heavy metals. The S. metallicus genome contained two complete cop gene clusters, each encoding a metallochaperone (CopM), a Cu-exporting ATPase (CopA), and a transcriptional regulator (CopT). Transcriptional expression analysis revealed that copM and copA from each cop gene cluster were cotranscribed and their transcript levels increased when S. metallicus was grown either in the presence of Cu or using chalcopyrite (CuFeS2) as oxidizable substrate. This study shows for the first time the presence of a duplicated version of the cop gene cluster in Archaea and characterizes some of the Cu and Cd resistance determinants in a thermophilic archaeon employed for industrial biomining.

show abstract

Section: Resultsmentioning

confidence: 99%

Molecular Characterization of Copper and Cadmium Resistance Determinants in the Biomining Thermoacidophilic ArchaeonSulfolobus metallicus

Orell

Remonsellez

Arancibia

et al. 2013

Archaea

View full text Add to dashboard Cite

show abstract

“…Archaeal genomes do not encode Rho‐like factors and archaeal NusA lacks the N‐terminal RNAP interaction domain. However, the two KH domains are highly conserved and it has recently been shown that the archaeal Aeropyrum pernix NusA binds to transcript sequences located at the 3′ terminus of rRNA with high affinity ( K d ∼60 nM; Shibata et al ., 2007). Transcription termination in the archaea is mediated by T‐rich sequences at the 3′ end of genes but appears to be independent of specific RNA secondary structures (Santangelo and Reeve, 2006).…”

Section: Post‐initiation Regulation Of Transcriptionmentioning

confidence: 99%

“…Archaeal genomes do not encode Rho-like factors and archaeal NusA lacks the N-terminal RNAP interaction domain. However, the two KH domains are highly conserved and it has recently been shown that the archaeal Aeropyrum pernix NusA binds to transcript sequences located at the 3′ terminus of rRNA with high affinity (K d~60 nM; Shibata et al, 2007).…”

Section: Post-initiation Regulation Of Transcriptionmentioning

confidence: 99%

Structure and function of archaeal RNA polymerases

Werner¹

2007

Molecular Microbiology

View full text Add to dashboard Cite

SummaryRNA polymerases (RNAPs) are essential to all life forms and therefore deserve our special attention. The archaeal RNAP is closely related to eukaryotic RNAPII in terms of subunit composition and architecture, promoter elements and basal transcription factors required for the initiation and elongation phase of transcription. RNAPs of this class are large and sophisticated enzymes that interact in a complex manner with DNA/RNA scaffolds, substrates NTPs and a plethora of transcription factors -interactions that often result in an allosteric regulation of RNAP activity. The 12 subunits of RNAP play distinct roles including RNAP assembly and stability, catalysis and functional contacts with exogenous factors. Due to the availability of structural information of RNAPs at high-resolution and wholly recombinant archaeal transcription systems, we are beginning to understand the molecular mechanisms of archaeal RNAPs and transcription in great detail.

show abstract

“…These stems presumably maintain the looped conformation to provide time for rRNA folding to complete before processing by dedicated RNases (12). The association of the KH domain with rRNA biogenesis is consistent with its distribution pattern: Archaea, but not eukaryotes, harbor a factor that displays high sequence identity to KH-KH repeats of bacterial NusA and similar spacerless head-to-tail packing (116). Intriguingly, the presumed loss of KH domains in eukaryotes coincides with the evolution of a specialized RNAP for rRNA synthesis and, perhaps more importantly, a principally different rRNA folding mechanism (124).…”

Section: Nus Factors and Rrna Antitermination Complexesmentioning

confidence: 62%

Regulation of Transcript Elongation

Belogurov

Artsimovitch

2015

Annu. Rev. Microbiol.

View full text Add to dashboard Cite

Bacteria lack subcellular compartments and harbor a single RNA polymerase that synthesizes both structural and protein-coding RNAs, which are cotranscriptionally processed by distinct pathways. Nascent rRNAs fold into elaborate secondary structures and associate with ribosomal proteins, whereas nascent mRNAs are translated by ribosomes. During elongation, nucleic acid signals and regulatory proteins modulate concurrent RNA-processing events, instruct RNA polymerase where to pause and terminate transcription, or act as roadblocks to the moving enzyme. Communications among complexes that carry out transcription, translation, repair, and other cellular processes ensure timely execution of the gene expression program and survival under conditions of stress. This network is maintained by auxiliary proteins that act as bridges between RNA polymerase, ribosome, and repair enzymes, blurring boundaries between separate information-processing steps and making assignments of unique regulatory functions meaningless. Understanding the regulation of transcript elongation thus requires genome-wide approaches, which confirm known and reveal new regulatory connections.

show abstract

Crystal structure and RNA-binding analysis of the archaeal transcription factor NusA

Cited by 17 publications

References 30 publications

Molecular Characterization of Copper and Cadmium Resistance Determinants in the Biomining Thermoacidophilic ArchaeonSulfolobus metallicus

Molecular Characterization of Copper and Cadmium Resistance Determinants in the Biomining Thermoacidophilic ArchaeonSulfolobus metallicus

Structure and function of archaeal RNA polymerases

Regulation of Transcript Elongation

Contact Info

Product

Resources

About