Crystal Structure and Interaction of Phycocyanin with &amp;#946;-Secretase: A Putative Therapy for Alzheimer’s Disease

Singh, Niraj Kumar; Hasan, Syed Shahzad; Kumar, Jitendra; Raj, Isha; Pathan, Amrin A.; Parmar, Asha; Shakil, Shazi; Gourinath, S.; Madamwar, Datta

doi:10.2174/1871527313666140228114456

Cited by 31 publications

(26 citation statements)

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“…Moreover, Singh et al has reported the crystal structure and in silico affinity of PC αβ dimer with β-secretase ( Figure 5), the potential drug target for Alzheimer's disease [41]. The same was also evidenced experimentally by using the Alzheimer model C. elegans CL4176 [41].…”

Section: Pbps As Anti-aging Moleculesmentioning

confidence: 65%

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Antioxidant Potential of Phycobiliproteins: Role in Anti-Aging Research

Sonani¹,

Rastogi²,

Madamwar³

2015

Biochem Anal Biochem

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Aging research has made significant progress over recent years, particularly after the formulation of 'Oxidative stress theory of aging'. According to this theory, aging and its associated abnormalities may be prevented, at least to some extent, by application of certain antioxidants. Cyanobacterial phycobiliproteins (PBPs), the major light harvesting pigment proteins are widely characterized for their in vivo and in vitro antioxidant activity. Since, reactive oxygen species (ROS) are considered as important factors to cause aging, PBPs can be used as an effective free radical scavengers and be a potent candidate to develop the anti-aging drug. The use of PBPs in preventing the oxidative stress mediated abnormalities or aging is rationally debated. The present review enlightens the recent advances in the field of antioxidant function of PBPs and major challenges in the application of these pigment proteins in anti-aging research. Also included is the possible mechanism behind the anti-aging capacity of these ecologically as well as economically important biomolecules.

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Section: Pbps As Anti-aging Moleculesmentioning

confidence: 65%

“…1FKN is found to dock in close proximity of the β-subunit while the 3UQP is found to dock in an α-subunit proximal location. (Adapted from Singh et al [41].…”

Section: Conclusion and Future Perspectivementioning

confidence: 99%

Antioxidant Potential of Phycobiliproteins: Role in Anti-Aging Research

Sonani¹,

Rastogi²,

Madamwar³

2015

Biochem Anal Biochem

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“…A09DM) in wild type and transgenic Caenorhabditis elegans (C. elegans) ( Figure 6). Singh et al [63] (2014) have recently given the putative therapy of Alzheimer's disease (AD) by in silico molecular docking between the structures of PC and βsecretase (molecule assumed to Sonani RR et al . Purification, production and applications of PBPs be possible drug target for AD) ( Figure 6C).…”

Section: Pharmaceutical and Neutraceuticalmentioning

confidence: 99%

“…A: Phycoerythrin-feeding increases the mean life span of wild type N2 C. elegans in dose-dependent manner [43] ; B: Phycoerythrin-feeding increases mean survival percentage of C. elegans in under normal and oxidative (paraquat) stressed conditions [43] ; C: PE-treatment moderates the heat-induced Alzheimer associated paralysis in the muscles of CL4176 C. elegans [63] ; D: Representative images of CL4176 animal grown at 16 ℃, at 25 ℃ and at 25 ℃ with phycoerythrin. Phycoerythrin effectively moderates the Alzheimer associated phenotype in CL4176 C. elegans [63] . PE: Phycoerythrin; C. elegans: Caenorhabditis elegans.…”

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confidence: 99%

Recent advances in production, purification and applications of phycobiliproteins

Sonani

Rastogi

Patel

et al. 2016

WJBC

151

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light-harvesting, PBPs are found to involve in several life sustaining phenomena including photoprotection in cyanobacteria. The unique spectral features (like strong absorbance and fluorescence), proteineous nature and, some imperative properties like hepato-protective, antioxidants, anti-inflammatory and anti-aging activity of PBPs enable their use in food, cosmetics, pharmaceutical and biomedical industries. PBPs have been also noted to show beneficial effect in therapeutics of some disease like Alzheimer and cancer. Such large range of applications increases the demand of PBPs in commodity market. Therefore, the large-scale and coast effective production of PBPs is the real need of time. To fulfil this need, many researchers have been working to find the potential producer of PBPs for the production and purification of PBPs. Results of these efforts have caused the inventions of some novel techniques like mixotrophic and heterotrophic strategies for production and aqueous two phase separation for purification purpose. Overall, the present review summarises the recent findings and identifies gaps in the field of production, purification and applications of this biological and economically important proteins. Core tip: The present review compiles the overall research that has been done so far in the field of produ ction, purification and application of phycobiliptoteins (PBPs). The comparative accounts for different methods for PBPs production, purification have been discussed. The potential applications of PBPs in various industries including food, cosmetics and pharmaceutical have been debated rationally. Remaining questions and rese arch gaps in respected field have been highlighted as conclusion. Recent advances in production, purification and applications of phycobiliproteins AbstractAn obligatory sunlight requirement for photosynthesis has exposed cyanobacteria to different quantity and quality of light. Cyanobacteria can exhibit efficient photosynthesis over broad region (450 to 650 nm) of solar spectrum with the help of brilliantly coloured pigment proteins called phycobiliproteins (PBPs). Besides REVIEW 100February 26, 2016|Volume 7|Issue 1|

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“…Especially, the phycobiliproteins (PBPs) are the major metabolic product and harvest the sunlight for photosynthesis in cyanobacteria. PBPs are widely used in food, cosmetics, and biomedical industries due to their unique color; amino acid composition; and other imperative biological properties including anticancer, antiinflammatory, and hepatoprotective activities (Sonani et al 2014aLiu et al 2000;Singh et al 2014;Kumar et al 2015;Rimbau et al 1999;Riss et al 2007;Romay et al 2003;Bei et al 2000).…”

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confidence: 99%

Phycoerythrin averts intracellular ROS generation and physiological functional decline in eukaryotes under oxidative stress

Sonani

Rastogi

Singh³

et al. 2016

Protoplasma

Self Cite

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In vitro antioxidant virtue and life-prolonging effect of phycoerythrin (PE; a pigment protein isolated from Phormidium sp. A09DM) have been revealed in our previous reports (Sonani et al. in Age 36:9717, 2014a; Sonani et al. in Process Biochem 49:1757-1766, 2014b). It has been hypothesized that the PE expands life span of Caenorhabditis elegans (bears large resemblance with human aging pathways) due to its antioxidant virtue. This hypothesis is tested in present study by checking the effect of PE on intracellular reactive oxygen species (ROS) generation and associated physiological deformities using mouse and human skin fibroblasts, C. elegans, and Drosophila melanogaster Oregon R and by divulging PE's structural attributes responsible for its antioxidant asset. PE treatment displayed noteworthy decrease of 67, 48, and 77 % in ROS level in mouse fibroblast (3T3-L1), human fibroblast, and C. elegans N2, respectively, arisen under chemical-induced oxidative stress. PE treatment delayed the development of paraquat-induced Alzheimer phenotype by 14.5 % in C. elegans CL4176. Furthermore, PE improved the locomotion of D. melanogaster Oregon R under oxidative stress with simultaneous up-regulation in super-oxide dismutase and catalase activities. The existence of 52 Glu + Asp + His + Thr residues (having metal ion sequestration capacity), 5 phycoerythrobilin chromophores (potential electron exchangers) in PE's primary structure, and significant hydrophobic patches on the surface of its α- and β-subunits are supposed to collectively contribute in the antioxidant virtues of PE. Altogether, results support the hypothesis that it is the PE's antioxidant asset, which is responsible for its life-prolonging effect and thus could be exploited in the therapeutics of ROS-associated abnormalities including aging and neurodegeneration in eukaryotes.

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Crystal Structure and Interaction of Phycocyanin with β-Secretase: A Putative Therapy for Alzheimer’s Disease

Cited by 31 publications

References 0 publications

Antioxidant Potential of Phycobiliproteins: Role in Anti-Aging Research

Antioxidant Potential of Phycobiliproteins: Role in Anti-Aging Research

Recent advances in production, purification and applications of phycobiliproteins

Phycoerythrin averts intracellular ROS generation and physiological functional decline in eukaryotes under oxidative stress

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