Crystal structure and enzyme engineering of the broad substrate spectrum l‐amino acid oxidase 4 from the fungus Hebeloma cylindrosporum
Simon Koopmeiners,
Dominic Gilzer,
Christiane Widmann
et al.
Abstract:l‐Amino acid oxidases (LAAOs) catalyze the oxidative deamination of l‐amino acids to α‐keto acids. Recombinant production of LAAOs with broad substrate spectrum remains a formidable challenge. We previously achieved this for the highly active and thermostable LAAO4 of Hebeloma cylindrosporum (HcLAAO4). Here, we crystallized a proteolytically truncated surface entropy reduction variant of HcLAAO4 and solved its structure in substrate‐free form and in complex with diverse substrates. The ability to support the a… Show more
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