Cryptosporidium parvum subtypes from diarrheic dairy calves in Israel

Yasur-Landau, Daniel; Zilberberg, Matan; Markovich, Michal Perry; Behar, Adi; Fleiderovitz, Ludmila; Mazuz, Monica L.

doi:10.1016/j.vprsr.2021.100608

Cited by 5 publications

(9 citation statements)

References 29 publications

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“…Cryptosporidium parvum was the most numerically predominant species present, representing 51.8% of total infections, followed by C. ryanae at 31.1% and C. bovis at 21.7%. Our results on the predominance of C. parvum are in agreement with those from surrounding regions on both healthy and diarrheic animals [31,35,36,[46][47][48]. The predominance of C. parvum specifically in pre-weaned cattle has also been reported in the USA [12].…”

Section: Discussionsupporting

confidence: 89%

“…The IIaA12G1R1 (orange, Figure 2 ) subtype comprised 25.4% of the C. parvum -positive samples and was only identified in a single farm. Previously, IIaA12G1R1 has been detected in cows in Israel, making this report the second one in the region [ 47 ]. The zoonotic potential of this subtype remains to be determined, having so far only been reported in animals.…”

Section: Discussionmentioning

confidence: 91%

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High Occurrence of Zoonotic Subtypes of Cryptosporidiumparvum in Cypriot Dairy Farms

et al. 2022

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Cryptosporidium parvum is one of the major causes of neonatal calf diarrhoea resulting in reduced farm productivity and compromised animal welfare worldwide. Livestock act as a major reservoir of this parasite, which can be transmitted to humans directly and/or indirectly, posing a public health risk. Research reports on the prevalence of Cryptosporidium in ruminants from east Mediterranean countries, including Cyprus, are limited. This study is the first to explore the occurrence of Cryptosporidium spp. in cattle up to 24 months old on the island of Cyprus. A total of 242 faecal samples were collected from 10 dairy cattle farms in Cyprus, all of which were screened for Cryptosporidium spp. using nested-PCR amplification targeting the small subunit of the ribosomal RNA (18S rRNA) gene. The 60 kDa glycoprotein (gp60) gene was also sequenced for the samples identified as Cryptosporidium parvum-positive to determine the subtypes present. The occurrence of Cryptosporidium was 43.8% (106/242) with at least one positive isolate in each farm sampled. Cryptosporidium bovis, Cryptosporidium ryanae and C. parvum were the only species identified, while the prevalence per farm ranged from 20‒64%. Amongst these, the latter was the predominant species, representing 51.8% of all positive samples, followed by C. bovis (21.7%) and C. ryanae (31.1%). Five C. parvum subtypes were identified, four of which are zoonotic—IIaA14G1R1, IIaA15G1R1, IIaA15G2R1 and IIaA18G2R1. IIaA14G1R1 was the most abundant, representing 48.2% of all C. parvum positive samples, and was also the most widespread. This is the first report of zoonotic subtypes of C. parvum circulating in Cyprus. These results highlight the need for further research into the parasite focusing on its diversity, prevalence, host range and transmission dynamics on the island.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 91%

High Occurrence of Zoonotic Subtypes of Cryptosporidiumparvum in Cypriot Dairy Farms

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Our results on predominance of C. parvum are in agreement with those from surrounding regions in both healthy and diarrheic animals [31,35,36,[46][47][48]. The predominance of C. parvum specifically in pre-weaned cattle has also been reported in the USA [12].…”

Section: Discussionsupporting

confidence: 89%

“…The IIaA12G1R1 (orange, Figure 2) comprised 25.4% of C. parvum positive samples and it was identified only in a single farm. Previously, IIaA12G1R1 was detected in cows in Israel, making this report the second one in the region [47]. Zoonotic potential of this subtype remains to be determined, having so far only been reported in animals.…”

Section: Discussionmentioning

confidence: 75%

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High Occurrence of Zoonotic Subtypes of <em>Cryptosporidium parvum</em> in Cypriot Dairy Farms

Hoque¹,

Mavrides²,

Pinto³

et al. 2022

Preprint

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Cryptosporidium parvum is one of the major causes of neonatal calf diarrhoea resulting in reduced farm productivity and compromised animal welfare worldwide. Livestock act as a major reservoir of this parasite, which can be transmitted to humans directly and/or indirectly, posing a public health risk. Research reports on Cryptosporidium prevalence from the east Mediterranean region are scarce with even less originating from Cyprus. This study is the first to explore occurrence of Cryptosporidium spp. in young dairy calves on the island. A total of 242 faecal samples were collected from 10 dairy cattle farms in Cyprus, all of which were screened for Cryptosporidium spp. using nested-PCR amplification targeting the small subunit of the ribosomal RNA (18S rRNA) gene. The 60 kDa glycoprotein (gp60) gene was also sequenced for the samples identified as C. parvum positive to determine the subtypes present. Occurrence of Cryptosporidium was 43.8% (106/242) with at least one positive isolate in each farm sampled. Prevalence per farm ranged from 20¬‒64%, while C. bovis, C. ryanae and C. parvum were the only species identified. Amongst those, the latter was the predominant species, representing 51.8% of all positive samples. Four zoonotic subtypes were identified – IIaA14G1R1, IIaA15G1R1, IIaA15G2R1 and IIaA18G2R1. IIaA14G1R1 was the most abundant; representing 48.2% of all C. parvum positive samples and was also the most widespread. This is the first report of zoonotic subtypes of C. parvum circulating in Cyprus. These results highlight the need for further research into the parasite focusing on its diversity, prevalence, host-range and transmission dynamics on the island.

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Cryptosporidium parvum gp60 subtypes in diarrheic lambs and goat kids from Israel

Tako,

Fleiderovitz,

Markovich

et al. 2023

Parasitol Res

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Cryptosporidium parvum subtypes from diarrheic dairy calves in Israel

Cited by 5 publications

References 29 publications

High Occurrence of Zoonotic Subtypes of Cryptosporidiumparvum in Cypriot Dairy Farms

High Occurrence of Zoonotic Subtypes of Cryptosporidiumparvum in Cypriot Dairy Farms

High Occurrence of Zoonotic Subtypes of <em>Cryptosporidium parvum</em> in Cypriot Dairy Farms

Cryptosporidium parvum gp60 subtypes in diarrheic lambs and goat kids from Israel

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