Cryptosporidium parvum Infection Depletes Butyrate Producer Bacteria in Goat Kid Microbiome

Mammeri, Mohamed; Obregón, Dasiel; Chevillot, Aurélie; Polack, Bruno; Julien, Christine; Pollet, Thomas V.; Cabezas-Cruz, Alejandro; Adjou, Karim

doi:10.3389/fmicb.2020.548737

Cited by 24 publications

(20 citation statements)

References 60 publications

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“…However, it should be noted that in the current study, the mice did not show clinical signs of cryptosporidiosis, making them possibly asymptomatic carriers of the parasite. However, in other studies involving goats, which showed mild to severe clinical symptoms of cryptosporidiosis (diarrhoea, hypothermia, growth retardation, and mortality), bacterial species associated with SCFA production have been shown to be depleted, thereby impacting overall SCFA biosynthesis pathways [31].…”

Section: Cryptosporidiosis Dynamics In the Gutmentioning

confidence: 96%

Cryptosporidiosis Modulates the Gut Microbiome and Metabolism in a Murine Infection Model

et al. 2021

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Cryptosporidiosis is a major human health concern globally. Despite well-established methods, misdiagnosis remains common. Our understanding of the cryptosporidiosis biochemical mechanism remains limited, compounding the difficulty of clinical diagnosis. Here, we used a systems biology approach to investigate the underlying biochemical interactions in C57BL/6J mice infected with Cryptosporidium parvum. Faecal samples were collected daily following infection. Blood, liver tissues and luminal contents were collected 10 days post infection. High-resolution liquid chromatography and low-resolution gas chromatography coupled with mass spectrometry were used to analyse the proteomes and metabolomes of these samples. Faeces and luminal contents were additionally subjected to 16S rRNA gene sequencing. Univariate and multivariate statistical analysis of the acquired data illustrated altered host and microbial energy pathways during infection. Glycolysis/citrate cycle metabolites were depleted, while short-chain fatty acids and D-amino acids accumulated. An increased abundance of bacteria associated with a stressed gut environment was seen. Host proteins involved in energy pathways and Lactobacillus glyceraldehyde-3-phosphate dehydrogenase were upregulated during cryptosporidiosis. Liver oxalate also increased during infection. Microbiome–parasite relationships were observed to be more influential than the host–parasite association in mediating major biochemical changes in the mouse gut during cryptosporidiosis. Defining this parasite–microbiome interaction is the first step towards building a comprehensive cryptosporidiosis model towards biomarker discovery, and rapid and accurate diagnostics.

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Section: Cryptosporidiosis Dynamics In the Gutmentioning

confidence: 96%

Cryptosporidiosis Modulates the Gut Microbiome and Metabolism in a Murine Infection Model

et al. 2021

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“…Studies comparing some of the methods (i.e., PICRUSt, PICRUSt2, and Tax4Fun) revealed that no method was superior to another [ 76 ], and PICRUSt2 has been applied on samples from various animals, including arthropods (e.g., Ixodes spp. [ 77 ], nematodes (e.g., Caenorhabditis elegans , [ 78 ]), birds (e.g., Serinus canaria domestica [ 79 ]), and mammals (e.g., humans [ 39 ], goats [ 80 ], and bats [ 21 ], among others. In our study, analysis of the functional prediction for multiple bat species, which are taxonomically different and have distinct feeding strategies, showed that frugivorous bats are very different from insectivorous, carnivores, or blood-feeders.…”

Section: Discussionmentioning

confidence: 99%

Structural differences in the gut microbiome of bats using terrestrial vs. aquatic feeding resources

et al. 2023

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Bat gut microbiomes are adapted to the specific diets of their hosts. Despite diet variation has been associated with differences in bat microbiome diversity, the influence of diet on microbial community assembly have not been fully elucidated. In the present study, we used available data on bat gut microbiome to characterize the microbial community assembly of five selected bat species (i.e., Miniopterus schreibersii, Myotis capaccinii, Myotis myotis, Myotis pilosus, and Myotis vivesi), using network analysis. These bat species with contrasting habitat and food preferences (i.e., My. capaccinii and My. pilosus can be piscivorous and/or insectivorous; Mi. schreibersii and My. myotis are exclusively insectivorous; while My. vivesi is a marine predator) offer an invaluable opportunity to test the impact of diet on bat gut microbiome assembly. The results showed that My. myotis showed the most complex network, with the highest number of nodes, while My. vivesi has the least complex structured microbiome, with lowest number of nodes in its network. No common nodes were observed in the networks of the five bat species, with My. myotis possessing the highest number of unique nodes. Only three bat species, My. myotis, My. pilosus and My. vivesi, presented a core microbiome and the distribution of local centrality measures of nodes was different in the five networks. Taxa removal followed by measurement of network connectivity revealed that My. myotis had the most robust network, while the network of My. vivesi presented the lowest tolerance to taxa removal. Prediction of metabolic pathways using PICRUSt2 revealed that Mi. schreibersii had significantly higher functional pathway’s richness compared to the other bat species. Most of predicted pathways (82%, total 435) were shared between all bat species, while My. capaccinii, My. myotis and My. vivesi, but no Mi. schreibersii or My. pilosus, showed specific pathways. We concluded that despite similar feeding habits, microbial community assembly can differ between bat species. Other factors beyond diet may play a major role in bat microbial community assembly, with host ecology, sociality and overlap in roosts likely providing additional predictors governing gut microbiome of insectivorous bats.

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“…C. parvum was orally administered to parasite-naïve goats, and infection was monitored for 26 days in fecal samples. C. parvum decreased the abundance of butyrate-producing pathways in bacteria and increased mucosal inflammation and tissue repair [ 110 ].…”

Section: Microbiota and Cryptosporidium Sppmentioning

confidence: 99%

Cryptosporidium: Still Open Scenarios

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Putignani

2022

Pathogens

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Cryptosporidiosis is increasingly identified as a leading cause of childhood diarrhea and malnutrition in both low-income and high-income countries. The strong impact on public health in epidemic scenarios makes it increasingly essential to identify the sources of infection and understand the transmission routes in order to apply the right prevention or treatment protocols. The objective of this literature review was to present an overview of the current state of human cryptosporidiosis, reviewing risk factors, discussing advances in the drug treatment and epidemiology, and emphasizing the need to identify a government system for reporting diagnosed cases, hitherto undervalued.

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Cryptosporidium parvum Infection Depletes Butyrate Producer Bacteria in Goat Kid Microbiome

Cited by 24 publications

References 60 publications

Cryptosporidiosis Modulates the Gut Microbiome and Metabolism in a Murine Infection Model

Cryptosporidiosis Modulates the Gut Microbiome and Metabolism in a Murine Infection Model

Structural differences in the gut microbiome of bats using terrestrial vs. aquatic feeding resources

Cryptosporidium: Still Open Scenarios

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