Cryptosporidium parvum GP60 subtypes in dairy cattle from Buenos Aires, Argentina

Coco, Valeria Fernanda Del; Córdoba, María Alejandra; Bilbao, G.; Castro, Aldana Pinto de Almeida; Basualdo, Juan Ángel; Fayer, Ronald; Santı́n, Mónica

doi:10.1016/j.rvsc.2013.12.010

Cited by 24 publications

(29 citation statements)

References 28 publications

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“…Cryptosporidium parvum infection in calves is widespread (Brook et al 2008;Trotz-Williams et al 2008;Al Mawly et al 2015;Qi et al 2015), and also in the present study, C. parvum was, in agreement with previous reports in Argentina, the only species identified in calves (Tomazic et al 2013;Del Coco et al 2014). However, in contrast to previous studies done in Argentina, a considerably higher overall prevalence was found (Del Coco et al 2008;Tiranti et al 2011;Garro et al 2016), probably due to an additional oocysts concentration step prior to microscopic examination significantly increasing the sensitivity of oocyst detection.…”

Section: Discussionsupporting

confidence: 90%

“…High genetic variability has been found in dairy calves in Argentina. Tomazic et al (2013) reported the six subtypes IIaA17G1R1, IIaA18G1R1, IIaA20G1R1, (Tomazic et al 2013, Del Coco et al 2014 England (2 of 51) (Brook et al 2009) The Netherlands (2 of 129) (Wielinga et al 2008) Sweden (3 of 171) Czech Republic (3 of 137) (Kváč et al 2011) Hungary ( (Tomazic et al 2013;Del Coco et al 2014). In England, this subtype has been also identified in humans (Chalmers et al 2011), strongly suggesting a zoonotic transmission risk.…”

Section: Dairy Herdmentioning

confidence: 99%

“…Molecular characterization of C. parvum at the subtype level in dairy calves, important to determine zoonotic potential, have been conducted in several countries (Santín et al 2008;Xiao and Fayer 2008;Amer et al 2010;Silverlås et al 2013;Smith et al 2014;Kaupke and Rzeżutka 2015). In Argentina, two previous studies have reported C. parvum as the only species present in 120 preweaned dairy calves and subtypes IIaA16G1R1 IIaA17G1R1, IIaA18G1R1, IIaA19G1R1, IIaA20G1R1, IIaA21G1R1, IIaA22G1R1, and IIaA23G1R1 have been identified (Tomazic et al 2013;Del Coco et al 2014). Whereas an association between subtypes and different clinical manifestations has been demonstrated in humans, few studies have tested the hypothesis that C. parvum-subtypes are associated with diarrhea intensity in dairy calves Insulander et al 2013;Adamu et al 2014).…”

Section: Introductionmentioning

confidence: 99%

“…Number of positive samples and the examined study group is given IIaA21G1R1, IIaA22G1R1, and IIaA23G1R1 from 45 dairy calves. DelCoco et al (2014) described the seven subtypes IIaA16G1R1, IIaA18G1R1, IIaA19G1R1, IIaA20G1R1, IIaA21G1R1, IIaA22G1R1, and IIaA23G1R1 from 73 dairy calves. The predominant subtype IIaA20G1R1 detected in this study, was also found with relatively high frequency in dairy calves studied in other areas of Argentina(Tomazic et al 2013;Del Coco et al 2014), apart from Sweden, Serbia, and Montenegro(Misic and Abe 2007).…”

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confidence: 99%

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Prevalence of Cryptosporidium parvum in dairy calves and GP60 subtyping of diarrheic calves in central Argentina

et al. 2019

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Cryptosporidiosis of calves is caused by the enteroprotozoan Cryptosporidium spp. The disease results in intense diarrhea of calves associated with substantial economic losses in dairy farming worldwide. The aim of this study was to determine calf, herd, and within-herd Cryptosporidium prevalence and identify Cryptosporidium species and subtypes in calves with diarrhea in intensive dairy herds in central Argentina. A total of 1073 fecal samples were collected from 54 randomly selected dairy herds. Cryptosporidium-oocysts were isolated and concentrated from fecal samples using formol-ether and detected by light microscopy with the modified Ziehl-Neelsen technique. Overall prevalence of oocyst-excreting calves was found to be 25.5% (274/ 1073) (95% C.I. 22.9; 28.1%). Of the herds studied, 89% (48/54) included at least one infected calf, whereas within-herd prevalence ranged from the absence of infection to 57% (20/35). A highly significant association was found between the presence of diarrhea and C. parvum infection (χ 2 = 55.89, p < 0.001). For species determination, genomic DNA isolated from oocystpositive fecal samples was subjected to PCR-RFLP of the 18S rRNA gene resulting exclusively in Cryptosporidium parvum identification. C. parvum isolates of calves displaying diarrhea and high rate of excretion of oocysts were subtyped by PCR amplification and direct sequencing of the 60 kDa glycoprotein (GP60) gene. Altogether five GP60 subtypes, designated IIaA18G1R1, IIaA20G1R1, IIaA21G1R1, IIaA22G1R1, and IIaA24G1R1 were identified. Interestingly, IIaA18G1R1 and IIaA20G1R1 were predominant in calves with diarrhea and high infection intensity. Notably, IIaA24G1R1 represents a novel, previously unrecognized C. parvum subtype. The subtype IIaA18G1R1, frequently found in this study, is strongly implicated in zoonotic transmission. These results suggest that calves might be an important source for human cryptosporidiosis in Argentina.

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Section: Discussionsupporting

confidence: 90%

Section: Dairy Herdmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

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Prevalence of Cryptosporidium parvum in dairy calves and GP60 subtyping of diarrheic calves in central Argentina

et al. 2019

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“…In our study, we identified two strains of the IIa family in 16 stool samples: strains IIaA20G1R1 and IIaA17G2R1, which not been reported in studies in this same country. The most common strain in our study, IIaA20G1R1, has been detected in cattle in some regions of the world: Serbia and Montenegro, Sweden, and Argentina [73,74,75,76], but there are no reports of this strain in humans. The second most common strain identified in stool samples, IIaA17G2R1, has been detected in other studies on cattle [77,78] and on humans [79,80].…”

Section: Discussionmentioning

confidence: 62%

Cryptosporidium spp. and Giardia spp. in feces and water and the associated exposure factors on dairy farms

et al. 2017

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The aims of this study were to verify the prevalence of Cryptosporidium spp. and Giardia spp. in animal feces and drinking water on dairy farms and to identify a possible relation between the exposure factors and the presence of these parasites. Fecal samples from cattle and humans and water samples were collected on dairy farms in Paraná, Brazil. Analysis of (oo)cysts in the feces was performed by the modified Ziehl-Neelsen staining and centrifugal flotation in zinc sulfate. Test-positive samples were subjected to nested PCR amplification of the 18SSU ribosomal RNA gene for identification of Cryptosporidium and Giardia and of the gp60 gene for subtyping of Cryptosporidium. Microbiological analysis of water was carried out by the multiple-tube method and by means of a chromogenic substrate, and parasitological analysis was performed on 31 samples by direct immunofluorescence and nested PCR of the genes mentioned above. Identification of the species of Cryptosporidium was performed by sequencing and PCR with analysis of restriction fragment length polymorphisms. The prevalence of Giardia and Cryptosporidium was higher in calves than in adults. Among the samples of cattle feces, Cryptosporidium parvum was identified in 41 (64%), C. ryanae in eight (12.5%), C. bovis in four (6.3%), C. andersoni in five (7.8%), and a mixed infection in 20 samples (31.3%). These parasites were not identified in the samples of human feces. Thermotolerant coliform bacteria were identified in 25 samples of water (45.5%). Giardia duodenalis and C. parvum were identified in three water samples. The gp60 gene analysis of C. parvum isolates revealed the presence of two strains (IIaA20G1R1 and IIaA17G2R2) in the fecal samples and one (IIaA17G2R1) in the water samples. The presence of coliforms was associated with the water source, structure and degradation of springs, rain, and turbidity. The prevalence of protozoa was higher in calves up to six months of age. C. parvum and G. duodenalis were identified in the water of dairy farms, as were thermotolerant coliforms; these findings point to the need for guidance on handling of animals, preservation of water sources, and water treatment.

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Multiple Cryptosporidium parvum subtypes detected in a unique isolate of a Chilean neonatal calf with diarrhea

et al. 2015

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To further understand the composition of population of parasite in a single host, we analyzed the GP60 gene of Cryptosporidium parvum amplified from DNA of a randomly selected isolate found in the feces of a diarrheic calf from a dairy farm in Central Chile. Direct sequencing of the amplicon yield the IIaA17G4R1 C. parvum subtype. The same amplicon was cloned in Escherichia coli (22 clones) and sequenced, yielding three different GP60 subtypes, IIaA17G4R1 (16/22), IIaA16G4R1 (1/22), and IIaA15G4R1 (1/22), and four sequences with nucleotide substitutions in the serine repeats, which subtype would be otherwise IIaA17G4R1. It is thus possible to determine allelic polymorphism using Sanger sequencing with an additional step of bacterial cloning. The results also indicate the necessity to further characterize parasite populations in a single host to better understand the dynamics of Cryptosporidium epidemiology.

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Cryptosporidium parvum GP60 subtypes in dairy cattle from Buenos Aires, Argentina

Cited by 24 publications

References 28 publications

Prevalence of Cryptosporidium parvum in dairy calves and GP60 subtyping of diarrheic calves in central Argentina

Prevalence of Cryptosporidium parvum in dairy calves and GP60 subtyping of diarrheic calves in central Argentina

Cryptosporidium spp. and Giardia spp. in feces and water and the associated exposure factors on dairy farms

Multiple Cryptosporidium parvum subtypes detected in a unique isolate of a Chilean neonatal calf with diarrhea

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