Cryptosporidium andersoni from a Danish cattle herd: identification and preliminary characterisation

Enemark, Heidi L.; Ahrens, Peter; Lowery, Colm; Thamsborg, Stig Milan; Enemark, J. M. D.; Bille‐Hansen, Vivi; Lind, Peter

doi:10.1016/s0304-4017(02)00083-3

Cited by 71 publications

(50 citation statements)

References 26 publications

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“…Contamination of the watershed presumably occurred either as a result of direct fecal inputs or through manure application with subsequent transportation of fecal contamination to waterways via tile drainage and overland flow. C. parvum, C. bovis, C. andersoni, and the deer-like genotype have been found to infect cattle (5,7,17,18,29,39), yet the data show widespread occurrence of C. andersoni with a limited occurrence of C. parvum and no observed occurrence of the other two. This may be explained by the sequential infection of cattle with various species and genotypes as they increase in age.…”

Section: Discussionmentioning

confidence: 68%

Tracking Host Sources of Cryptosporidium spp. in Raw Water for Improved Health Risk Assessment

Ruecker

Braithwaite

Topp

et al. 2007

Appl Environ Microbiol

109

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Recent molecular evidence suggests that different species and/or genotypes of Cryptosporidium display strong host specificity, altering our perceptions regarding the zoonotic potential of this parasite. Molecular forensic profiling of the small-subunit rRNA gene from oocysts enumerated on microscope slides by U.S. Environmental Protection Agency method 1623 was used to identify the range and prevalence of Cryptosporidium species and genotypes in the South Nation watershed in Ontario, Canada. Fourteen sites within the watershed were monitored weekly for 10 weeks to assess the occurrence, molecular composition, and host sources of Cryptosporidium parasites impacting water within the region. Cryptosporidium andersoni, Cryptosporidium muskrat genotype II, Cryptosporidium cervine genotype, C. baileyi, C. parvum, Cryptosporidium muskrat genotype I, the Cryptosporidium fox genotype, genotype W1, and genotype W12 were detected in the watershed. The molecular composition of the Cryptosporidium parasites, supported by general land use analysis, indicated that mature cattle were likely the main source of contamination of the watershed. Deer, muskrats, voles, birds, and other wildlife species, in addition to sewage (human or agricultural) may also potentially impact water quality within the study area. Source water protection studies that use land use analysis with molecular genotyping of Cryptosporidium parasites may provide a more robust source-tracking tool to characterize fecal impacts in a watershed. Moreover, the information is vital for assessing environmental and human health risks posed by water contaminated with zoonotic and/or anthroponotic forms of Cryptosporidium.

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Section: Discussionmentioning

confidence: 68%

Tracking Host Sources of Cryptosporidium spp. in Raw Water for Improved Health Risk Assessment

Ruecker

Braithwaite

Topp

et al. 2007

Appl Environ Microbiol

109

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“…Thomaz et al (2007) found 88.9% of C. parvum and 11.1% of C. bovis in cattle, whereas Huber et al (2005) found C. parvum only. In other regions of the world C. parvum and C. andersoni were the two most common genotypes in bovines (XIAO; HERD, 1994;WADE et al, 2000;HUETINK et al, 2001;ENEMARK et al, 2002;PENG et al, 2003). In Maryland, United States, C. parvum accounted for 97% of infections in pre-weaned calves but only 4 and 0% of infections in post-weaned calves and heifers, respectively (SANTÍN et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

Occurrence and molecular characterization of Cryptosporidium spp. isolated from domestic animals in a rural area surrounding Atlantic dry forest fragments in Teodoro Sampaio municipality, State of São Paulo, Brazil

Sevá

Funada

Souza

et al. 2010

Rev. Bras. Parasitol. Vet.

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The aim of this study was to assess the occurrence of Cryptosporidium in domestic animals in rural properties surrounding rain forest fragments within the municipality of Teodoro Sampaio, southeastern Brazil. Conventional sucrose flotation method followed by molecular characterization of the parasites by sequencing PCR products amplified from SSU rRNA gene were used. Stool samples were collected from domestic animals raised as pets and livestock in all rural properties surrounding three forest fragments. Samples from cattle (197), equine (63), pigs (25), sheep (11), and dogs (28) were collected from 98 rural properties. The frequency of occurrence of Cryptosporidium within each animal species was 3.0% (6/197) among cattle and 10.7% (3/28) among dogs. Cryptosporidium was not detected in stool samples from equine, sheep, and pigs. All sequences obtained from the six samples of calves showed molecular identity with Cryptosporidium andersoni while all sequences from dog samples were similar to C. canis. The frequency of occurrence of Cryptosporidium in these domestic animal species was low. The absence of C. parvum in the present study suggests that the zoonotic cycle of cryptosporidiosis may not be relevant in the region studied. The presence of Cryptosporidium species seldom described in humans may be, otherwise, important for the wild fauna as these animals are a source of infection and dissemination of this protozoan to other animal species. The impact and magnitude of infection by C. andersoni in wild ruminants and C. canis in wild canids have to be assessed in future studies to better understand the actual importance of these species in this region.

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“…oocysts (natural infections). No lambs shed Cryptosporidium oocysts by DPI 28 (primary infection), which was longer than the prepatent period (25 days) experimentally verified in a calf (Enemark et al 2002). All experimental lambs were reinfected with Cryptosporidium oocysts but all faecal staining proved negative during the length of the experiment.…”

mentioning

confidence: 97%

Failed attempt of Cryptosporidium andersoni infection in lambs

Kváč¹,

Ditrich²,

Kouba³

et al. 2004

FOLIA PARASIT

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Abstract. Four lambs 4 months old were inoculated with Cryptosporidium andersoni oocysts (bovine isolate) in a dose of 5 × 10 6 per animal followed later by 1 × 10 7 oocysts per animal. No animal shed Cryptosporidium oocysts during 56 days post infection, no lamb showed clinical symptoms of cryptosporidiosis and no macroscopic changes were detected in the abomasum immediately after autopsy. Histological examinations did not demonstrate the occurrence of Cryptosporidium in the abomasum and other selected organs.

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Cryptosporidium andersoni from a Danish cattle herd: identification and preliminary characterisation

Cited by 71 publications

References 26 publications

Tracking Host Sources of Cryptosporidium spp. in Raw Water for Improved Health Risk Assessment

Tracking Host Sources of Cryptosporidium spp. in Raw Water for Improved Health Risk Assessment

Occurrence and molecular characterization of Cryptosporidium spp. isolated from domestic animals in a rural area surrounding Atlantic dry forest fragments in Teodoro Sampaio municipality, State of São Paulo, Brazil

Failed attempt of Cryptosporidium andersoni infection in lambs

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