Cryptosporangium eucalypti sp. nov., an actinomycete isolated from Eucalyptus camaldulensis roots

Abstract

A novel actinomycete, designated strain EURKPP3H10T, was isolated from surface-sterilized roots of Eucalyptus camaldulensis Dehnh., collected from Kamphaengphet Silvicultural Research Station, Kamphaengphet province, Thailand. The taxonomic position of strain EURKPP3H10T was studied using a polyphasic approach. Phylogenetic evaluation based on 16S rRNA gene sequence analysis showed that strain EURKPP3H10T belongs to the genus Cryptosporangium, with the highest sequence similarity to Cryptosporangium cibodasense LIPI11-2-Ac046T (99.2 %). Colonies of strain EURKPP3H10T were orange yellow. Spherical sporangia with motile spores were observed. The strain contained meso-diaminopimelic acid and acofriose, arabinose, galactose, glucose, mannose, xylose and ribose in whole-cell hydrolysates. The predominant menaquinones were MK-9(H8) and MK-9(H6). The major fatty acids were iso-C16 : 0, C17 : 1ω8c, C18 : 1ω9c and C17 : 0. The polar lipids of the strain were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol and unknown lipids. The DNA G+C content of the genomic DNA was 71.5 mol%. Based on comparative analysis of physiological, biochemical and chemotaxonomic data, including DNA-DNA hybridization, strain EURKPP3H10T represents a novel species of the genus Cryptosporangium, for which the name Cryptosporangium eucalypti sp. nov. is proposed. The type strain is EURKPP3H10T (=BCC 77605T=NBRC 111482T).

Taxonomic and Metabolite Diversities of Moss-Associated Actinobacteria from Thailand

“…Among the seven genera, Cryptosporangium spp. has also been isolated from plant litter [102,103] or as endophytes of plants [104]. Due to the close phylogenetic relationships among known species and between many of our samples and those known species, the final taxonomic placements of most of our isolates, especially the yellow-highlighted ones, such as S3-21 and S3-31, in Figure 6, await further investigation using either multilocus sequence typing or whole-genome sequencing.…”

Streptomyces silvisoli sp. nov., a polyene producer, and Streptomyces tropicalis sp. nov., two novel actinobacterial species from peat swamp forests in Thailand

“…A 0.1 ml aliquot of the various dilutions was spread on humic acid–vitamin agar [9], final pH 7.0, supplemented with nalidixic acid (25 µg ml −1 ) and nystatin (50 µg ml −1 ). After incubation at 28 °C for 14 days, colonies of the strains were isolated and purified on glucose–yeast extract agar (GYEA) [10]. Mycelia and spores of the strains were preserved in sterile glycerol solution (20 % v/v) at −20 °C.…”

Kitasatospora humi sp. nov., isolated from a tropical peat swamp forest soil, and proposal for the reclassification of Kitasatospora psammotica as a later heterotypic synonym of Kitasatospora aureofaciens

“…A 0.1 ml aliquot of the various dilutions was spread on humic acid-vitamin agar [5], final pH 7.0, supplemented with nalidixic acid (25 µg ml −1 ) and nystatin (50 µg ml −1 ). After the plates were incubated at 28 °C for 14 days, the single colony of strain RB6PN24 T was transferred and purified on glucose-yeast extract agar (GYEA) [6]. The strain was subsequently maintained and preserved on GYEA slant at 30 °C.…”