Cryptogein and capsicein promote defence responses in Quercus suber against Phytophthora cinnamomi infection

Medeira, C.; Quartin, V. L.; Maia, I.; Diniz, Inês; Matos, Marlene; Semedo, José N.; Campos, Paula Scotti; Ramalho, José C.; Pais, Isabel P.; Ramos, Paula Acácia Silva; Melo, Elvira; Leitão, António E.; Cravador, A.

doi:10.1007/s10658-012-9972-x

Cited by 12 publications

(14 citation statements)

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“…In addition, recent cytological and physiological studies showed that cryptogein (elicitin secreted by Phytophthora cryptogea) and capsicein (secreted by Phytophthora capsici) induced a resistance response on cork oak against infection by P. cinnamomi (Medeira et al 2012a) and that α-CIN has the same effect in Castanea sativa (Medeira et al 2012b). In the present work we show that α-CIN induces a defence response to the infection by P. cinnamomi in Q. suber and in Q. ilex as well.…”

Section: Discussionsupporting

confidence: 68%

“…Their action is not genus-or cultivar-specific and not only elicitins from Phytophthora but also from Pythium, like oligandrin play this role. Plants in the family Fagaceae as shown here and in other reports (Medeira et al 2012a andMedeira et al 2012b) using various approaches, display defence responses induced by elicitins.…”

Section: Discussionsupporting

confidence: 66%

“…Previous cytohistological observations have shown that P. cinnamomi colonizes Q. suber and Q. ilex penetrating to the phloem and xylem vessels and leading to occlusion, at least in 2-to 6-month-old plants (Pires et al 2008;Horta et al 2010;Medeira et al 2012a;Ruiz-Gómez et al 2014). In addition, recent cytological and physiological studies showed that cryptogein (elicitin secreted by Phytophthora cryptogea) and capsicein (secreted by Phytophthora capsici) induced a resistance response on cork oak against infection by P. cinnamomi (Medeira et al 2012a) and that α-CIN has the same effect in Castanea sativa (Medeira et al 2012b).…”

Section: Discussionmentioning

confidence: 99%

“…More recently it has been reported in cork oak that P. cinnamomi hyphae spread inter-and intracellularly throughout the cortical parenchyma and invaded the vascular cylinder. Hyphae also filled the xylem and phloem vessels, leading to their occlusion (Medeira et al 2012a;Horta et al 2010).…”

Section: Introductionmentioning

confidence: 99%

“…However, oligandrin, an elicitin-like protein secreted by Pythium oligandrum, was shown to induce systemic resistance to Fusarium oxysporum and Botrytis cinerea in tomato and grapevine, respectively (Benhamou et al 2001;Mohamed et al 2007). Also, recent histological and ultra-structural studies (Medeira et al 2012a(Medeira et al , 2012b, assessing of changes in fatty acid composition of roots and leaves, and gas exchanges and fluorescence analysis (Medeira et al 2012a) have documented that cryptogein and capsicein can trigger cellular responses in cork oak roots (Medeira et al 2012a) and α-cinnamomin (α-CIN) in Castanea sativa roots (Medeira et al 2012b) during P. cinnamomi infection. This strongly suggests that a resistance defence arose against the pathogen upon elicitin treatment.…”

Section: Introductionmentioning

confidence: 99%

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Induction of defence responses by cinnamomins against Phytophthora cinnamomi in Quercus suber and Quercus ilex subs. rotundifolia

et al. 2015

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The strong association between Phytophthora cinnamomi and the mortality and decline of Quercus suber and Q. ilex subsp. rotundifolia has been known for two decades. The ability of elicitins secreted by this pathogen to trigger defence responses in these Quercus against itself was evaluated in this work. Biomass quantification by quantitative real-time PCR revealed a significant decrease in pathogen colonization of Q. suber roots after 24 h pre-treatment with α-and β-cinnamomin. In Q. suber and Q. ilex roots pre-treated with α-cinnamomin, hyphae were unable to reach and colonize the vascular cylinder and showed cytoplasmic disorganization in all the roots observed as contrasted with non-pre-treated roots. The pathogen was restricted to the intercellular spaces of the cortical parenchyma and the concomitant accumulation of electron dense materials was observed in contact with the hyphae. Furthermore, ROS (reactive oxygen species) production and the enzymatic activities of superoxide dismutase, catalase and peroxidase were compared in infected and non-infected Quercus roots in time course trials. There was a significant increase in the production of hydrogen peroxide (H2O2) and superoxide anion (O •-) and an enhanced activity of the enzymes in infected roots was observed at each time point. When comparing with elicitin non-treated roots, the α-cinnamomin-treated roots in interaction with P. cinnamomi showed a decrease in ROS accumulation and an increase of the enzyme activities. The overall results were consistent with an induction by the cinnamomins which initiated defence responses against the pathogen invasion of roots. Finally, elicitins were immunolocalized in the contact zone of P. cinnamomi hyphae with epidermal host cells, plasmalemma outer cytoplasm and around the intracellular hyphae in the vacuoles of invaded epidermal cells.

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Section: Discussionsupporting

confidence: 68%

Section: Discussionsupporting

confidence: 66%