Cryptogein affects expression of α3, α6 and β1 20S proteasome subunits encoding genes in tobacco

Abstract

Twelve alpha and beta 20S proteasome subunits cDNAs showing 70-82% identity with the corresponding genes in Arabidopsis or rice, and features of eukaryotic proteasome subunits were cloned in tobacco. Only beta1-tcI 7, alpha3 and alpha6, 20S proteasome subunits encoding genes were up-regulated by cryptogein, a proteinaceous elicitor of plant defence reactions. These results led to the hypothesis that the activation of beta1-tcI 7, alpha3 and alpha6 could induce a specific proteolysis involved in the hypersensitive response and systemic acquired resistance monitored by cryptogein.

Tomato susceptibility to Fusarium crown and root rot: Effect of grafting combination and proteomic analysis of tolerance expression in the rootstock

“…Proteasome subunit a type-3 and 6 are components of the 20S plant proteasome complex, which is involved in many cellular processes (Baharami and Gray, 1999), both developmental and adaptative, including defense responses (Becker et al, 2000). Interestingly, it has been shown that the fungal elicitor cryptogein triggers a-3 and 6 proteasome subunit expression in tobacco (Dahan et al, 2001), thus suggesting that specific proteolytic events may be involved in the expression of the plant response to the pathogen. In our conditions, levels of a 3 and 6 proteasome subunits were increased only in the susceptible interaction.…”

Ubiquitination during Plant Immune Signaling

“…In tobacco, expression of three genes encoding subunits of the 20S proteasome (a3, a6, b1) is induced after treatment with the elicitor cryptogein (Dahan et al, 2001;Suty et al, 2003). Tobacco cell lines overexpressing the b1 subunit showed a drastic reduction of the NtRbohD (NADPH oxidase) gene induction and of its associated oxidative burst after cryptogein treatment, suggesting that the b1 subunit acts as a negative regulator of early plant responses to cryptogein (Lequeu et al, 2005).…”

Protein Polyubiquitination Plays a Role in Basal Host Resistance of Barley

“…First, several genes encoding pathway components like E2 or RING E3 ligases have been found to be transcriptionally upregulated by pathogen-derived elicitors (Dahan et al, 2001;Takai et al, 2002). Second, the RPM1 (for resistance to Pseudomonas syringae pv maculicola 1) resistance protein in Arabidopsis thaliana has been found to become destabilized upon pathogen attack, suggesting enhanced proteasome-mediated turnover (Boyes et al, 1998).…”