Cryptococcus neoformans strains isolated from church towers in Rio de Janeiro City, RJ, Brazil

Baroni, Francisco de Assis; Paula, Claudete Rodrigues; Silva, Eriques Gonçalves da; Viani, Flávio Cesar; Rivera, Irma Nelly Gutierrez; Oliveira, Maria Tereza Barreto de; Gambale, Walderez

doi:10.1590/s0036-46652006000200003

Cited by 48 publications

(53 citation statements)

References 29 publications

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“…Oligonucleotides of the microsatellite-specific sequence (GACA) 4 were used as single primers in the PCR, as described by MEYER et al 38 . The amplification reactions were performed in a volume of 25 µL, using the Ready-to-Go RAPD Kit according to the manufacturer's instructions.…”

Section: Rapd and Pcr-fingerprintingmentioning

confidence: 99%

“…The PLB1-RFLP and (GACA) 4 -PCR fingerprinting analysis were carried out to separate the strains of C. neoformans into eight major molecular types ( Fig. 2A).…”

Section: Plb1-rflp Pcr-fingerprinting and Rapd Analysismentioning

confidence: 99%

“…In Brazil, from 1980 to 2002, 215,810 AIDS patients were registered, among whom 6% had cryptococcosis at the time of diagnosis 9 and São Paulo is the state with the highest rate of this disease. The majority of the Brazilian clinical and environmental isolates have shown to be serotype A with exception in north and northeastern regions where the serotype B is endemic 1,4,41,46 .…”

Section: Introductionmentioning

confidence: 99%

“…Several molecular typing methods have been used in epidemiological analyses of clinical and/or environmental isolates of C. neoformans, including karyotyping, PCR fingerprinting, random amplified polymorphic DNA (RAPD), restriction fragment length polymorphism (RFLP) and amplified fragment length polymorphism (AFLP) 5,7,8,10,12,16,22,26,37,45 . PCR-fingerprinting using mini (M13) or microsatellite [(GACA) 4 ] specific sequences as single primers and RFLP (PLB1 and URA5 genes) methods have grouped global isolates of C. neoformans into eight major molecular types 32,37,38 . Likely, BOEKHOUT et al using AFLP analysis grouped into six major AFLP groups 8 .…”

Section: Introductionmentioning

confidence: 99%

“…In light of this, we analyzed clinical isolates of C. neoformans from several regions of São Paulo State to evaluate serotypes, genetic variability using PCRfingerprinting with (GACA) 4 oligonucleotide, RAPD with primer 6, PLB1-RFLP and mating type analysis by PCR.…”

Section: Introductionmentioning

confidence: 99%

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Genotyping, serotyping and determination of mating-type of Cryptococcus neoformans clinical isolates from São Paulo State, Brazil

Matsumoto

Fusco‐Almeida

Baeza

et al. 2007

Rev. Inst. Med. trop. S. Paulo

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SUMMARYThe basidiomycetous yeast Cryptococcus neoformans is an important fungal pathogen mainly in immunocompromised patients. In this study, 47 clinical isolates of C. neoformans from regions of São Paulo State were studied serologically by using the Crypto Check Iatron RM 304-K kit, their genetic diversity was estimated by PCR-fingerprinting with a microsatellite-specific sequence (GACA) 4 , RAPD with primer 6 (Amersham Pharmacia Biotech), PCR-restriction fragment length polymorphism (RFLP) analysis of the phospholipase B gene (PLB1) digested with AvaI and mating type analysis by PCR. All 47 strains isolated from HIV positive patients included in this study were serotype A and MATα. The majority of the isolates (45/47) were VNI and only two were VNII by PCR-fingerprinting and PCR-RFLP analysis. High degree of homogeneity was observed when (GACA) 4 was used, being highly correlated (> 0.9). In contrast, the RAPD analysis was more heterogeneous with higher number of molecular profiles. By PCR-RFLP, no new molecular type was found, enhancing the suggestion that the differences based on conserved gene as PLB1, can be resultant of ongoing divergent evolution within the C. neoformans complex, into the current eight subtypes. Our results furnish new information on the molecular epidemiology of C. neoformans in the southeast region of Brazil.

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Section: Rapd and Pcr-fingerprintingmentioning

confidence: 99%

“…The PLB1-RFLP and (GACA) 4 -PCR fingerprinting analysis were carried out to separate the strains of C. neoformans into eight major molecular types ( Fig. 2A).…”

Section: Plb1-rflp Pcr-fingerprinting and Rapd Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Genotyping, serotyping and determination of mating-type of Cryptococcus neoformans clinical isolates from São Paulo State, Brazil

Matsumoto

Fusco‐Almeida

Baeza

et al. 2007

Rev. Inst. Med. trop. S. Paulo

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Mycoses in HIV/AIDS Patients

Shen

Liu

Pan

et al. 2020

Mucocutaneous Manifestations of HIV/AIDS

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First Environmental Isolations of Cryptococcus neoformans and Cryptococcus gattii in Tunisia and Review of Published Studies on Environmental Isolations in Africa

Mseddi¹,

Sellami²,

Jarboui³

et al. 2011

Mycopathologia

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Cryptococcus neoformans and Cryptococcus gattii are pathogenic yeasts that cause cryptococcosis. These fungi were commonly associated with pigeon droppings and plant materials. The habitat of these pathogens has not been yet studied in Tunisia, although the ecology of these yeasts must be elucidated in order to establish surveillance programs and to prevent infections. The aim of this survey was to recover C. neoformans and C. gattii environmental isolates from pigeon droppings and plant materials in different areas of Sfax region, Tunisia. Nine hundred and fifty samples from leaves, wood, flowers, fruits and soil around trunk bases of 40 almond (Prunus dulcis) and 60 eucalyptus trees were collected as well as 250 pigeon droppings samples from different sites: buildings (n = 150), houses (n = 50) and zoo (n = 50). The identification of Cryptococcus neoformans complex was confirmed using the ID32C auxanogram panel (BioMérieux, Marcy l'Etoile, France); species were determined by multiplex PCR using the CN70 and CN49 primers, and mating type was determined by PCR. C. neoformans was recovered from 26 specimens of pigeon droppings (10.4%). This yeast was obtained more frequently from dry droppings (9.2%) than from moist droppings (1.2%). The mating type was determined. All the 31 environmental strains of C. neoformans and C. gattii were MATα. Out of 700 samples tested from 100 trees, only 5 isolates of Cryptococcus neoformans species complex were recovered (0.6%), two isolates of C. gattii and one isolate of C. neoformans were recovered from the wood of E. camaldulensis trees, and only two isolates of C. gattii were recovered from the wood of almond trees (Prunus dulcis Mill. var. zaaf and var. achek). These two Tunisian almond tree varieties were recorded for the first time in Africa as hosts for C. gattii. These results add new information to the ecology and epidemiology of C. neoformans species complex in Tunisia.

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Cryptococcus neoformans strains isolated from church towers in Rio de Janeiro City, RJ, Brazil

Cited by 48 publications

References 29 publications

Genotyping, serotyping and determination of mating-type of Cryptococcus neoformans clinical isolates from São Paulo State, Brazil

Genotyping, serotyping and determination of mating-type of Cryptococcus neoformans clinical isolates from São Paulo State, Brazil

Mycoses in HIV/AIDS Patients

First Environmental Isolations of Cryptococcus neoformans and Cryptococcus gattii in Tunisia and Review of Published Studies on Environmental Isolations in Africa

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