Cryptochrome mediated magnetic sensitivity in Arabidopsis occurs independently of light-induced electron transfer to the flavin

Hammad, Merfat; Albaqami, Maria; Pooam, Marootpong; Kernevez, E.; Witczak, Jacques; Ritz, Thorsten; Martino, Carlos F.; Ahmad, Margaret

doi:10.1039/c9pp00469f

Cited by 51 publications

(65 citation statements)

References 47 publications

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“…The static magnetic field exposure conditions are essentially as previously described [28,29], with the exception that all experiments were conducted in the dark, and in the CO 2 incubator. The Control condition was the culture of HEK293 exposed to the local magnetic fields inside the CO 2 incubator (40 μT).…”

Section: Static Magnetic Field and Low-level Field Exposure Conditionsmentioning

confidence: 99%

“…The higher intensity static magnetic fields (500 μT and 2 mT) were generated by a square Helmholtz coil (20 cm per side) placed inside the incubator as described previously [23,28,29]. Each coil consisted of 20 turns of copper wire with the two coils separated by 11.5 cm.…”

Section: Static Magnetic Field and Low-level Field Exposure Conditionsmentioning

confidence: 99%

“…To generate the low-level static field exposure (LLF), the samples were placed within two concentric μ-metal cylinders with walls 0.2 mm thick, as described previously [29]. The inner cylinder was 11.5 cm diameter, and the outer one was 16 cm diameter, and both were 30 cm in height.…”

Section: Static Magnetic Field and Low-level Field Exposure Conditionsmentioning

confidence: 99%

“…was suggested by the fact that cryptochrome, a putative static magnetic field receptor, had been implicated in PEMF responses [23,31], and by the fact that the static magnetic fields emitted transiently by PEMF devices occur within a range of intensities known to have physiological consequences [28][29][30]. Our initial expectation was that exposure to the higher magnetic fields (0.5 and 2 mT) might result in an enhanced biological response as compared to exposure to the earth's geomagnetic field (40 μT).…”

Section: Plos Onementioning

confidence: 99%

“…Intriguingly, an evolutionarily conserved flavoprotein receptor known as cryptochrome [27], also undergoes redox chemistry thought compatible with the Radical Pair Mechanism for magnetic sensitivity [28]. Cryptochrome has been linked to static field magnetosensing in many biological systems [28][29][30], and has also been implicated in sensitivity to PEMF exposure in human HEK cells [23] and mouse organotypic brain culture [31].…”

Section: Introductionmentioning

confidence: 99%

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HEK293 cell response to static magnetic fields via the radical pair mechanism may explain therapeutic effects of pulsed electromagnetic fields

Pooam

Jourdan²,

Esawi

et al. 2020

PLoS ONE

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PEMF (Pulsed Electromagnetic Field) stimulation has been used for therapeutic purposes for over 50 years including in the treatment of memory loss, depression, alleviation of pain, bone and wound healing, and treatment of certain cancers. However, the underlying cellular mechanisms mediating these effects have remained poorly understood. In particular, because magnetic field pulses will induce electric currents in the stimulated tissue, it is unclear whether the observed effects are due to the magnetic or electric component of the stimulation. Recently, it has been shown that PEMFs stimulate the formation of ROS (reactive oxygen species) in human cell cultures by a mechanism that requires cryptochrome, a putative magnetosensor. Here we show by qPCR analysis of ROS-regulated gene expression that simply removing cell cultures from the Earth’s geomagnetic field by placing them in a Low-Level Field condition induces similar effects on ROS signaling as does exposure of cells to PEMF. This effect can be explained by the so-called Radical Pair mechanism, which provides a quantum physical means by which the rates and product yields (e.g. ROS) of biochemical redox reactions may be modulated by magnetic fields. Since transient cancelling of the Earth’s magnetic field can in principle be achieved by PEMF exposure, we propose that the therapeutic effects of PEMFs may be explained by the ensuing modulation of ROS synthesis. Our results could lead to significant improvements in the design and therapeutic applications of PEMF devices.

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Section: Static Magnetic Field and Low-level Field Exposure Conditionsmentioning

confidence: 99%

Section: Static Magnetic Field and Low-level Field Exposure Conditionsmentioning

confidence: 99%

Section: Static Magnetic Field and Low-level Field Exposure Conditionsmentioning

confidence: 99%

Section: Plos Onementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

HEK293 cell response to static magnetic fields via the radical pair mechanism may explain therapeutic effects of pulsed electromagnetic fields

Pooam

Jourdan²,

Esawi

et al. 2020

PLoS ONE

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The Effects of Uniform and Nonuniform Magnetic Fields in Plant Growth: A Meta‐Analysis Approach

Tapia-Belmonte

Concha

Poupin

2023

Bioelectromagnetics

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Magnetic field (MF) effects have been reported in plants' growth, seed germination, gene expression, and water consumption. Accordingly, magnetic treatments have been proposed as a sustainable alternative to improve yields. Nevertheless, a comprehensive quantitative assessment is needed to understand whether their effects are general, species‐specific, or dependent on the experimental setting. We conducted a multilevel meta‐analysis of 45 articles that studied 29 different plant species. A positive and neutral effect of a nonuniform MF was found on fresh weight and germination rate, respectively. A significant association was found between a uniform MF and germination. These results suggest that MFs improve plant growth. However, the effects are highly dependent on the experimental setting. This opens exciting questions about the biophysical mechanisms underlying the perception and transduction of this environmental cue and about the possible translation to agricultural practices. © 2023 Bioelectromagnetics Society.

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A novel strategy of selective gene delivery by using a uniform magnetic field

Dateki

Imamura

Arai

et al. 2021

Biotechnology Journal

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The application of a magnetic field to enhance the transfection efficiency has been reported to be mainly dependent on the magnetic force generated by a magnetic field gradient to attract paramagnetic bead-conjugated carrier and polynucleotide complexes. This strategy has the advantage of targeting a point or an area on the culture vessel. However, it is difficult to target deeply placed tissues in vivo. Uniform magnetic field-correlated effect is applicable to such a purpose. Here, we attempted to establish a novel procedure for uniform magnetic field-dependent enhancement of transfection efficiency. We examined the effect of a 1.5 mT uniform magnetic field on cellular reactive oxygen species (ROS) level and transfection efficiency mediated by a ROS-sensitive transfection carrier. Our experimental results revealed that a 1.5 mT uniform magnetic field transiently decreased cellular ROS levels and strongly enhanced transfection efficiency mediated by polyethylenimine (PEI). The uniform magnetic field-dependent enhancement of PEI-mediated in vivo transfection was confirmed in the livers of mice. Local intensification of a uniform magnetic field in a culture dish resulted in selective gene delivery into cells on the target area. Although further examination and improvement are necessary for this procedure, our findings provide a novel option for spatial control of gene delivery. K E Y W O R D S polyethylenimine, reactive oxygen species, selective gene delivery, transfection, uniform magnetic field 1 INTRODUCTION To analyze the physiological roles of the genes of interest, researchers experience growing demands for selective gene delivery. Techniques for photodynamic control of DNA/RNA delivery have become available and possess great potential for future applications in research Abbreviations: 8-OHdG, 8-hydroxy-2'-deoxyguanosine; AIPcS 2a , aluminum phthalocyanine disulfonate; CBA, cell-based assay; DAPI, 4' ,6-diamidino-2-phenylindole; eGFP, enhanced green fluorescent protein; ES, embryonic stem; N/P, nitrogen/phosphate; NAC, n-acetyl cysteine; PBS, phosphate-buffered saline; PEI, polyethylenimine; qPCR, quantitative polymerase chain reaction; qRT-PCR, quantitative reverse transcription PCR; ROS, reactive oxygen species; SEM, standard error of mean and therapy. DNA/RNA-nanocarrier complexes enter cells mainly by clathrin-mediated endocytosis. Endocytic trafficking of the complexes includes the following four stages: cell binding, endocytosis, endosomal escape, and cytosolic transit. [1] Essential for the efficient DNA/RNA delivery among these stages is the endosomal escape, which avoids lysosomal degradation. Photodynamic enhancement of endosomal escape mediated by photosensitizers and light irradiation results in large improvements in transfection efficiency. [2-7] The strategy of photodynamic enhancement has disadvantages because of poor light transmission through tissues. In contrast, magnetic fields can penetrate living organs and initiate cellular and molecular reactions. [8] It has been shown that the onset of...

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Cryptochrome mediated magnetic sensitivity in Arabidopsis occurs independently of light-induced electron transfer to the flavin

Abstract: Arabidopsis cryptochrome-dependent magnetosensitivity occurs via a reaction that does not require light. This excludes radical pairs formed during light-triggered electron transfer to the flavin.

Cited by 51 publications

References 47 publications

HEK293 cell response to static magnetic fields via the radical pair mechanism may explain therapeutic effects of pulsed electromagnetic fields

HEK293 cell response to static magnetic fields via the radical pair mechanism may explain therapeutic effects of pulsed electromagnetic fields

The Effects of Uniform and Nonuniform Magnetic Fields in Plant Growth: A Meta‐Analysis Approach

A novel strategy of selective gene delivery by using a uniform magnetic field

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