Cryptochrome 1 regulates growth and development in <i><scp>B</scp>rassica</i> through alteration in the expression of genes involved in light, phytohormone and stress signalling

Sharma, Pooja; Chatterjee, Mithu; Burman, Naini; Khurana, Jitendra P.

doi:10.1111/pce.12212

Cited by 52 publications

(36 citation statements)

References 118 publications

(287 reference statements)

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“…In the same study, using overexpression, it was shown that phototropin (PHOT2) was involved in blue light-induced anthocyanin accumulation. Also, cryptochromes (CRY1 and CRY2) control the blue lightinduced anthocyanin accumulation response [39,40]. In the present study, the highest CBG and THC concentrations were measured in the NS1 treatment, which had the highest portion of blue and UV-A wavelengths in the spectrum compared to the other treatments.…”

Section: Plant Morphology and Flower Yieldmentioning

confidence: 58%

The Effect of Light Spectrum on the Morphology and Cannabinoid Content of Cannabis sativa L.

Magagnini

Grassi

Kotiranta³

2018

Med Cannabis Cannabinoids

103

117

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Cannabis sativa L. flowers are the main source of Δ-9-tetrahydrocannabinol (THC) used in medicine. One of the most important growth factors in cannabis cultivation is light; light quality, light intensity, and photoperiod play a big role in a successful growth protocol. The aim of the present study was to examine the effect of 3 different light sources on morphology and cannabinoid production. Cannabis clones were grown under 3 different light spectra, namely high-pressure sodium (HPS), AP673L (LED), and NS1 (LED). Light intensity was set to ∼450 µmol/m 2 /s measured from the canopy top. The photoperiod was 18L: 6D/21 days during the vegetative phase and 12L: 12D/46 days during the generative phase, respectively. At the end of the experiment, plant dry weight partition, plant height, and cannabinoid content (THC, cannabidiol [CBD], tetrahydrocannabivarin [THCV], cannabigerol [CBG]) were measured under different light treatments. The experiment was repeated twice. The 3 light treatments (HPS, NS1, AP673L) resulted in differences in cannabis plant morphology and in cannabinoid content, but not in total yield of cannabinoids. Plants under HPS treatment were taller and had more flower dry weight than those under treatments AP673L and NS1. Treatment NS1 had the highest CBG content. Treatments NS1 and AP673L had higher CBD and THC concentrations than the HPS treatment. Results were similar between experiments 1 and 2. Our results show that the plant morphology can be manipulated with the light spectrum. Furthermore, it is possible to affect the accumulation of different cannabinoids to increase the potential of medicinal grade cannabis. In conclusion, an optimized light spectrum improves the value and quality of cannabis. Current LED technology showed significant differences in growth habit and cannabinoid profile compared to the traditional HPS light source. Finally, no difference of flowering time was observed under different R:FR (i.e., the ratio between red and far-red light).

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Section: Plant Morphology and Flower Yieldmentioning

confidence: 58%

The Effect of Light Spectrum on the Morphology and Cannabinoid Content of Cannabis sativa L.

Magagnini

Grassi

Kotiranta³

2018

Med Cannabis Cannabinoids

103

117

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“…Similar to that found in Arabidopsis, decreased or increased expression of BnCRY1 results in decreased or increased blue light sensitivity in the light inhibition of hypocotyl elongation [60]. Overexpression of BnCRY1 also caused enhanced anthocyanin accumulation, increased expansion in cotyledons and leaves, as well as reduced internode elongation of the stem [146]. Furthermore, BnCRY1 overexpression alters the mRNA abundance of genes associated with phytohormones and stress responses, arguing a broader effect of BnCRY1 in plant growth [146].…”

Section: Physiological Responses Mediated By Plant Cryptochromesmentioning

confidence: 83%

Cryptochrome-Mediated Light Responses in Plants

Wang

Nguyen

et al. 2014

Signaling Pathways in Plants

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Cryptochromes (CRY) are photolyase-like flavoproteins that have been found in all evolutionary lineages. Plant and animal cryptochromes are no longer DNA repairing enzymes but they apparently gained other biochemical functions in evolution. Plant cryptochromes are UV-A/blue light photoreceptors and play a pivotal role in plant growth and development, whereas animal cryptochromes act as either photoreceptors or transcription regulators. The first cryptochrome gene was isolated from Arabidopsis thaliana, which regulate stem growth, flowering time, stomatal opening, circadian clock, and other light responses. Cryptochromes are also found in all major crops investigated, with additional functions discovered, such as seed germination, leaf senescence, and stress responses. In this chapter, we will review some aspects of cryptochrome-mediated light responses in plants. Readers are referred to other review articles for photochemistry and signal transduction mechanism of plant cryptochromes [1-3].

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“…The supernatant was centrifuged again at 13,000 rpm and 4°C, and the concentration of proteins was estimated by the Bradford assay (Bradford, 1976). Equal concentrations of proteins were loaded on SDS-PAGE gels, and the protein extracts were subjected to western blotting (Sharma et al, 2014). For detection of OsbZIP48 protein, peptide antibody synthesis was done.…”

Section: Western-blot Analysismentioning

confidence: 99%

“…Southern analysis was carried out as described by Sharma et al (2014) with some modifications. A 10-mg aliquot of genomic DNA from each transgenic line, vector control, and the wild-type plant was restriction digested using EcoRI (Roche Molecular Bio Labs) and probed with the hptII gene cassette (850 bp).…”

Section: Southern Analysismentioning

confidence: 99%

OsbZIP48, a HY5 Transcription Factor Ortholog, Exerts Pleiotropic Effects in Light-Regulated Development

2017

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Plants have evolved an intricate network of sensory photoreceptors and signaling components to regulate their development. Among the light signaling components identified to date, HY5, a basic leucine zipper (bZIP) transcription factor, has been investigated extensively. However, most of the work on HY5 has been carried out in Arabidopsis (Arabidopsis thaliana), a dicot. In this study, based on homology search and phylogenetic analysis, we identified three homologs of AtHY5 in monocots; however, AtHYH (HY5 homolog) homologs are absent in the monocots analyzed. Out of the three homologs identified in rice (Oryza sativa), we have functionally characterized OsbZIP48. OsbZIP48 was able to complement the Athy5 mutant. OsbZIP48 protein levels are developmentally regulated in rice. Moreover, the OsbZIP48 protein does not degrade in dark-grown rice and Athy5 seedlings complemented with OsbZIP48, which is in striking contrast to AtHY5. In comparison with AtHY5, which does not cause any change in hypocotyl length when overexpressed in Arabidopsis, the overexpression of full-length OsbZIP48 in rice transgenics reduced the plant height considerably. Microarray analysis revealed that OsKO2, which encodes ent-kaurene oxidase 2 of the gibberellin biosynthesis pathway, is down-regulated in OsbZIP48OE and up-regulated in OsbZIP48 KD transgenics as compared with the wild type. Electrophoretic mobility shift assay showed that OsbZIP48 binds directly to the OsKO2 promoter. The RNA interference lines and the T-DNA insertional mutant of OsbZIP48 showed seedling-lethal phenotypes despite the fact that roots were more proliferative during early stages of development in the T-DNA insertional mutant. These data provide credible evidence that OsbZIP48 performs more diverse functions in a monocot system like rice in comparison with its Arabidopsis ortholog, HY5.

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Cryptochrome 1 regulates growth and development in Brassica through alteration in the expression of genes involved in light, phytohormone and stress signalling

Cited by 52 publications

References 118 publications

The Effect of Light Spectrum on the Morphology and Cannabinoid Content of Cannabis sativa L.

The Effect of Light Spectrum on the Morphology and Cannabinoid Content of Cannabis sativa L.

Cryptochrome-Mediated Light Responses in Plants

OsbZIP48, a HY5 Transcription Factor Ortholog, Exerts Pleiotropic Effects in Light-Regulated Development

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