Cryptic speciation in Triatoma sordida (Hemiptera: Reduviidae) from the Bolivian Chaco

Abstract

Eight natural Bolivian populations of two closely related species of Triatominae, Triatoma sordida and T. guasayana, were analysed by multilocus enzyme electrophoresis at 20 loci. Both species were readily separated and no natural hybrid was observed. Among the silvatic sample of T. sordida, strong departure from panmixia within and between loci was detected in two sites of the Chaco, suggesting two reproductively separate populations easily recognized at Idh2 and Mdh2 loci. Genetic distance between them was in agreement with the hypothesis of distinct species. However, the detection of 3% of putative hybrids suggested a recent evolutionary divergence.

Chromosome homogeneity in populations of Triatoma brasiliensis Neiva 1911 (Hemiptera - Reduviidae - Triatominae)

“…We detected an extensive polymorphism of C-heterochromatin in natural populations of Triatoma infestans and Triatoma sordida . In the latter species, the chromosome differences led to the revalidation of Triatoma garciabesi, subsequently confirmed by isoenzymatic (Noireau et al, 1998) and morphometric ( Jurberg et al, 1998) markers. However, in T. brasiliensis, we could not detect any cytogenetic differences among specimens with distinct color patterns.…”

Triatoma vandae sp. n. do complexo oliveirai encontrada no estado de Mato Grosso, Brasil (Hemiptera: Reduviidae: Triatominae)

“…e 6-fosfogluconate desidrogenase (6-PGDH, EC 1.1.1.44.). As condições de migração e coloração são derivadas de Richardson et al (1986) modificadas por Noireau et al (1998).…”

Mitochondrial DNA variation of Triatoma infestans populations and its implication on the specific status of T. melanosoma

“…However, discrepancies may arise in the case of sibling (Lent et al 1993, Panzera et al 1997, Noireau et al 1998), or polytypic species (Monteiro et al 1998. Recently, information coming from the employment of molecular tools is increasing our knowledge of the specific status in the Triatominae.…”

“…Thus we decide to undertake a more comprehensive analysis of these two closely related species employing other morphologic and molecular (cytogenetics and allozymes) methods that have also been successfully used to investigate taxonomic questions in the Triatominae (e.g. Panzera et al 1995, Noireau et al 1998. Additionally, to further clarify their status, in terms of genetic compatibility, T. melanosoma and T. infestans were submitted to cross-mating experiments.…”