Cryptic Anuran Biodiversity in Bangladesh Revealed by Mitochondrial 16S rRNA Gene Sequences

Hasan, Mahmudul; Islam, Mohammed Mafizul; Khan, Md. Mukhlesur Rahman; Alam, Mohammad Shafiqul; Kurabayashi, Atsushi; Igawa, Takeshi; Kuramoto, Mitsuru; Sumida, Masayuki

doi:10.2108/zsj.29.162

Cited by 45 publications

(75 citation statements)

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“…Several researchers (Kosuch et al, 2001;Kurabayashi et al, 2005;Frost et al, 2006;Sumida et al, 2007;Kotaki et al, 2010;Hasan et al, 2012a) have argued that members of the genus Fejervarya could be separated into 2 distinct groups-Southeast and South Asian-based on molecular data. This view was further supported by researchers who clearly showed complete hybrid inviability between these 2 groups by crossing experiments Djong et al, 2007;Islam et al, 2008b).…”

Section: Discussionmentioning

confidence: 99%

“…2.3. PCR and sequencing PCR amplification was performed using a primer set of 12S_3´end_Fow1 and 12S_3´end Fow2 (Hasan et al, 2012a). The primers were also used for DNA sequencing.…”

Section: Dna Extractionmentioning

confidence: 99%

“…The primers were also used for DNA sequencing. We used the following additional primers for sequencing when necessary: F51 and R51 (Sumida et al, 2002), 16S_R530 and 16S_R723 (Hasan et al, 2012a), R1051 (5´-ATGTTTTTGGTAAACAGGCGGG-3´), FR60 (5´-AAGCAAGTCGTAACATGGTA-3´), 16S_Rev1_SMA (5´-GGTTATTGTTAAGCTTTAACGC-3´), and 16S_Rev2_ CANCRI (5´-GAGAAATTAAGCTTTAACGC-3´). The length of the resultant 16S fragments varied from 1287 to 1354 bp between taxa.…”

Section: Dna Extractionmentioning

confidence: 99%

“…PCR mixtures were prepared with the TaKaRa Ex Taq Kit (Takara Bio, Inc., Shiga, Japan), according to the manufacturer's protocol. DNA amplification and sequencing strategies were followed according to the procedure by Hasan et al (2012a), and PCR products were purified using MicroSpin S-300 HR columns (GE Healthcare, Buckinghamshire, UK). The amplified 16S segments were directly sequenced on both strands using the BigDye Terminator Cycle Sequencing Kit (ABI) with an automated DNA sequencer (3100-Avant; ABI, Brooklyn, NY, USA).…”

Section: Dna Extractionmentioning

confidence: 99%

“…Although it is not sufficient to define a biological species on the basis of molecular data alone (Ferguson, 2002), the utility of molecular data in taxonomic studies (e.g., Tautz et al, 2003), especially in searching for cryptic species (i.e. 2 or more species sharing similar external morphology) (e.g., Bickford et al, 2007 [review]; Hasan et al, 2012a;Mila et al, 2012), has been demonstrated. One such procedure is called the "candidate species approach" (Vences et al, 2005a;Fouquet et al, 2007;Vieites et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

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Genetic divergences of South and Southeast Asian frogs: a case study of several taxa based on 16S ribosomal RNA gene data with notes on the generic name Fejervarya

Hasan¹,

ISLAM²,

KHAN³

et al. 2014

Turk J Zool

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Section: Discussionmentioning

confidence: 99%

“…2.3. PCR and sequencing PCR amplification was performed using a primer set of 12S_3´end_Fow1 and 12S_3´end Fow2 (Hasan et al, 2012a). The primers were also used for DNA sequencing.…”

Section: Dna Extractionmentioning

confidence: 99%

Section: Dna Extractionmentioning

confidence: 99%

Section: Dna Extractionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Genetic divergences of South and Southeast Asian frogs: a case study of several taxa based on 16S ribosomal RNA gene data with notes on the generic name Fejervarya

Hasan¹,

ISLAM²,

KHAN³

et al. 2014

Turk J Zool

Self Cite

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An insight into molecular taxonomy of bufonids, microhylids, and dicroglossid frogs: First genetic records from Pakistan

Akram

Rais

Lopez-Hervas

et al. 2021

Ecology and Evolution

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The current study was focused on documentation of amphibian assemblage in North Punjab and Islamabad Capital Territory, Pakistan, by using mitochondrial gene sequences of 16S rRNA. Our study entailed 37% of the known amphibian species of the country. We provided a phylogenetic analysis based on 74 newly generated mitochondrial 16S rRNAs from nine species of genus Microlyla, Duttaphrynus, Allopaa, Nanorana, Sphaerotheca, Minervarya, Hoplobatrachus, and Euphlyctis. We employed the maximum-likelihood inference and Bayesian analysis to assess the taxonomic status of the samples obtained from Pakistan, with respect to other congeneric species from surrounding regions. Our findings confirmed the taxonomic status of South Asian anuran species Duttaphrynus stomaticus, Duttaphrynus melanostictus, Microhyla nilphamariensis, Allopaa hazarensis, Nanorana vicina, Sphaerotheca maskeyi (synonym: S. pashchima), Minervarya pierrei, Hoplobatrachus tigerinus, and Euphlyctis kalasgramensis in Pakistan. We have reported new country records of genus Minervarya (M. pierrei).Minervarya pierrei was previously misidentified as Fejervarya limnocharis, due to dearth of genetic information. We provided the first genetic records of our endemic species N. vicina. The results revealed the taxonomic placement of N. vicina with respect to its congeners and validated the taxonomic status of N. vicina from its type locality (Murree) for the first time. The findings of the present study also indicated the paraphyletic relationship of A.-hazarensis with Nanorana species. So, based on our phylogenetic inferences, morphological characters, and habitat preferences, validity of generic status of A. hazarensis is undecided. As our data were not enough to resolve this issue, we suggest sequencing of additional mitochondrial and nuclear genes in the future studies to get a better resolution. We recommend carrying out extensive surveys throughout the country for proper scientific documentation of amphibians of Pakistan. Many new species, some of them might be endemic to Pakistan, are expected to be discovered, and taxonomic status of other species would be resolved.

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Postmating isolation and evolutionary relationships among Fejervarya species from Lesser Sunda, Indonesia and other Asian countries revealed by crossing experiments and mtDNA Cytb sequence analyses

Hasan

Kurniawan

Soewondo

et al. 2022

Ecology and Evolution

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To evaluate the degree of postmating isolation and the evolutionary relationships among frog species in the genus Fejervarya from Indonesia (Lesser Sunda), Bangladesh, China, and Japan, crossing experiments and molecular phylogenetic analyses were carried out. Crossing experiments revealed that reciprocal hybrids among F. iskandari , F. verruculosa , and F . sp. large type and between F. multistriata and F. kawamurai are viable through metamorphosis, while those between the F. iskandari group and F. limnocharis group were completely or partially inviable at the tadpole stage and those between Southeast Asian and South Asian Fejervarya groups were completely inviable at the embryonic stage. The mature reciprocal hybrids between F. iskandari and F. verruculosa from Lesser Sunda, Indonesia, showed some degree of abnormality in spermatogenesis. In phylogenetic analyses based on mtDNA Cytb sequences, F. iskandari formed a sister clade with F. verruculosa from Lesser Sunda, Indonesia, with 8.1% sequence divergence. F. multistriata from China formed a clade with populations of F. limnocharis in Thailand, Malaysia, and Indonesia (topotype), and these taxa showed sister relationships to F. kawamurai from Japan with 8.9% sequence divergence. Fejervarya sp. small type from Bangladesh formed a clade with the other South Asian members of the Fejervarya group and formed a sister clade with the Southeast Asian Fejervarya group, with 23.1% sequence divergence in the Cytb gene. These results showed that the degree of postmating isolation reflects molecular phylogenetic relationships and that F. iskandari and F. verruculosa from Indonesia (Lesser Sunda) are reproductively isolated by abnormalities in spermatogenesis and show genetic differentiation.

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Cryptic Anuran Biodiversity in Bangladesh Revealed by Mitochondrial 16S rRNA Gene Sequences

Cited by 45 publications

References 50 publications

Genetic divergences of South and Southeast Asian frogs: a case study of several taxa based on 16S ribosomal RNA gene data with notes on the generic name Fejervarya

Genetic divergences of South and Southeast Asian frogs: a case study of several taxa based on 16S ribosomal RNA gene data with notes on the generic name Fejervarya

An insight into molecular taxonomy of bufonids, microhylids, and dicroglossid frogs: First genetic records from Pakistan

Postmating isolation and evolutionary relationships among Fejervarya species from Lesser Sunda, Indonesia and other Asian countries revealed by crossing experiments and mtDNA Cytb sequence analyses

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