Crypthecodinium cohnii Growth and Omega Fatty Acid Production in Mediums Supplemented with Extract from Recycled Biomass

Didrihsone, Elina; Dubencovs, Konstantīns; Grūbe, Māra; Shvirksts, Karlis; Šuleiko, Anastasija; Šuleiko, Artūrs; Vanags, Juris

doi:10.3390/md20010068

Cited by 10 publications

(7 citation statements)

References 47 publications

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“…Additionally, ethanol could be taken up simultaneously with glycerol (Figure 2). The early-stage accumulation of PUFAs in the C. cohnii biomass, cultivated on each of the three carbon sources, was monitored by FTIR spectroscopy, following the approach used in Didrihsone et al [21]. FTIR was chosen as a rapid analytical method, requiring a small sample size and no complex pretreatment steps.…”

Section: Comparison Of Growth Substrate Consumption and Accumulation ...mentioning

confidence: 99%

Kinetic and Stoichiometric Modeling-Based Analysis of Docosahexaenoic Acid (DHA) Production Potential by Crypthecodinium cohnii from Glycerol, Glucose and Ethanol

et al. 2022

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Docosahexaenoic acid (DHA) is one of the most important long-chain polyunsaturated fatty acids (LC-PUFAs), with numerous health benefits. Crypthecodinium cohnii, a marine heterotrophic dinoflagellate, is successfully used for the industrial production of DHA because it can accumulate DHA at high concentrations within the cells. Glycerol is an interesting renewable substrate for DHA production since it is a by-product of biodiesel production and other industries, and is globally generated in large quantities. The DHA production potential from glycerol, ethanol and glucose is compared by combining fermentation experiments with the pathway-scale kinetic modeling and constraint-based stoichiometric modeling of C. cohnii metabolism. Glycerol has the slowest biomass growth rate among the tested substrates. This is partially compensated by the highest PUFAs fraction, where DHA is dominant. Mathematical modeling reveals that glycerol has the best experimentally observed carbon transformation rate into biomass, reaching the closest values to the theoretical upper limit. In addition to our observations, the published experimental evidence indicates that crude glycerol is readily consumed by C. cohnii, making glycerol an attractive substrate for DHA production.

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Section: Comparison Of Growth Substrate Consumption and Accumulation ...mentioning

confidence: 99%

Kinetic and Stoichiometric Modeling-Based Analysis of Docosahexaenoic Acid (DHA) Production Potential by Crypthecodinium cohnii from Glycerol, Glucose and Ethanol

et al. 2022

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“…Crypthecodinium cohnii CCMP 316 was obtained from the Provasoli-Guillard National Center for Marine Algae and Microbiota (NCMA) (East Boothbay, ME, USA). Inoculum cultures were grown as previously described by Didrihsone et al [14] in 250 mL bottles with a working volume of 150 mL, a medium containing 5 g glucose L −1 (Sigma-Aldrich Co., Ltd., St. Louis, MO, USA, Prod. # 49159), 2 g yeast extract L −1 (Sigma-Aldrich Co., Ltd., St. Louis, MO, USA, Prod.…”

Section: Inoculum Preparationmentioning

confidence: 99%

“…Sample aliquots were pipetted on a 384-well microplate, dried, and recorded in the wavenumber range of 4000-600 cm −1 with a spectral resolution of 4 cm −1 ; 64 scans were co-added. Omega FAs were identified based on absorption bands at ~1743 cm −1 and ~3012 cm −1 [14,18,19]. Only spectra with absorbance within absorption limits between 0.25 and 0.80 (where the concentration of a component is proportional to the intensity of the absorption band) were used for data analysis.…”

Section: Fourier Transform Infrared Spectrometrymentioning

confidence: 99%

Investigation of Crypthecodinium cohnii High-Cell-Density Fed-Batch Cultivations

Dubencovs,

Suleiko,

Suleiko

et al. 2024

Fermentation

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Crypthecodinium cohnii is a marine microalga that can accumulate high amounts of polyunsaturated fatty acids (PUFAs) and thus replace conventional routes of fish oil production. They are associated with the destruction of marine resources and multiple downstream/purification complications. The major drawbacks of using C. cohnii for industrial-scale production are associated with low PUFA productivity. One of the means of increasing the PUFA synthesis rate is to maintain the medium component concentrations at optimal values throughout cultivation, thus increasing PUFA production efficiency, which can result in the successful transfer of the process to pilot and/or industrial scale. The goal of the present research was to develop techniques for increasing the efficiency of PUFA production via C. cohnii cultivation. Multiple experiments were carried out to test and fine-tune the cultivation medium composition and oxygen transfer factors. The biomass yields from individual components, yeast extract, sea salts, and glucose amounted to 5.5, 0.65, and 0.61 g·g−1, respectively. C. cohnii cell susceptibility to mechanical damage was experimentally evaluated. Power inputs of <276.5 W/m3 did not seem to promote cell destruction when Pitched-blade impellers were used. The obtained cultivation conditions were shown to be efficient in terms of increasing the biomass productivity and the omega-3 fatty acid content in C. cohnii. By using the applied methods, the maximal biomass productivity reached 8.0 g·L−1·day−1, while the highest obtained biomass concentration reached 110 g·L−1. A steady increase in the concentration of PUFAs during cultivation was observed from the FTIR data.

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“…These values can be potentially achieved by means of genetic engineering [71]. A more realistic scenario of 0.06 g/L/h and 0.14 g/L/h and 50% oil content without genetic engineering [72] is also depicted in Figure 16. CAPEX and OPEX is shown in Figure 17.…”

Section: Cohnii Biorefinery Techno-economic Analysismentioning

confidence: 99%

Cascading Crypthecodinium cohnii Biorefinery: Global Warming Potential and Techno-Economic Assessment

et al. 2022

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Prior to the commissioning of a new industrial biorefinery it is deemed necessary to evaluate if the new project will be beneficial or detrimental to climate change, one of the main drivers for the sustainable development goals (SDG) of the United Nations. In particular, how SDG 7, Clean and Efficient Energy, SDG 3, Good Health and Well Being, SDG 9, Industry Innovation and Infrastructure, and SDG 12, Responsible Production and Consumption, would engage in a new biorefinery design, beneficial to climate change, i.e., fostering SDG 13, Climate Action. This study uses life cycle assessment methodology (LCA) to delve in detail into the Global Warming Impact category, project scenario GHG savings, using a conventional and a dynamic emission flux approach until 2060 (30-year lifetime). Water, heat and electricity circularity are in place by using a water recirculation process and a combined heat and power unit (CHP). A new historical approach to derive low and higher-end commodity prices (chemicals, electricity, heat, jet/maritime fuel, DHA, N-fertilizer) is used for the calculation of the economic indicators: Return of investment (ROI) and inflation-adjusted return (IAR), based upon the consumer price index (CPI). Main conclusions are: supercritical fluid extraction is the hotspot of energy consumption; C. cohnii bio-oil without DHA has higher sulfur concentration than crude oil based jet fuel requiring desulfurization, however the sulfur levels are compatible with maritime fuels; starting its operation in 2030, by 2100 an overall GHG savings of 73% (conventional LCA approach) or 85% (dynamic LCA approach) is projected; economic feasibility for oil productivity and content of 0.14 g/L/h and 27% (w/w) oil content, respectively (of which 31% is DHA), occurs for DHA-cost 100 times higher than reference fish oil based DHA; however future genetic engineering achieving 0.4 g/L/h and 70% (w/w) oil content (of which 31% is DHA), reduces the threshold to 20 times higher cost than reference fish oil based DHA; N-fertilizer, district heating and jet fuel may have similar values then their fossil counterparts.

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Crypthecodinium cohnii Growth and Omega Fatty Acid Production in Mediums Supplemented with Extract from Recycled Biomass

Cited by 10 publications

References 47 publications

Kinetic and Stoichiometric Modeling-Based Analysis of Docosahexaenoic Acid (DHA) Production Potential by Crypthecodinium cohnii from Glycerol, Glucose and Ethanol

Kinetic and Stoichiometric Modeling-Based Analysis of Docosahexaenoic Acid (DHA) Production Potential by Crypthecodinium cohnii from Glycerol, Glucose and Ethanol

Investigation of Crypthecodinium cohnii High-Cell-Density Fed-Batch Cultivations

Cascading Crypthecodinium cohnii Biorefinery: Global Warming Potential and Techno-Economic Assessment

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