Cryosurvival after exposure of IVF-derived Nellore embryos to different cryoprotectants and exposure times during vitrification

Souza, J. F.; Lienou, Landry Lienou; Rodrigues, Ana Paula Ribeiro; Alexandrino, Emerson; Cavalcante, Tânia Vasconcelos; Santos, Regiane R.; Figueiredo, J.R.; Dias, Francisca Elda Ferreira

doi:10.1016/j.cryobiol.2018.08.009

Cited by 4 publications

(2 citation statements)

References 13 publications

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“…Moreover, the type of cryoprotectants used for vitrification may also impact embryo implantation potential. While sucrose, dimethyl sulfoxide (DMSO) and 1,2-propanediol (PROH), are ubiquitously used and have been reported to have no detrimental impact on the viability and morphology of oocytes and embryos [32][33][34] , there are contradictory studies that suggest that DMSO and PROH lead to premature centromere separation in murine oocytes and consequent embryo aneuploidy [35][36][37][38] . Furthermore, the concentration of cryoprotectants employed in clinical settings varies depending on the commercial vitrification kits used, such as: Vitrification Kit (Kitazato BioPharma, Shizuoka, Japan) 39 , Sage Vitrification Kit (Origio, Denmark) 40 and Rapid-I devices (Vitrolife) 41 .…”

Section: Discussionmentioning

confidence: 99%

Impact of repeated cryopreservation on embryo health and implantation potential

Li,

Chow

et al. 2023

Preprint

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In IVF clinics, preimplantation genetic testing (PGT) is a common practice that involves a biopsy and cryopreservation of embryos for genetic evaluation. When testing fails or is required for already cryopreserved embryos, multiple freeze-thaw cycles occur. Though known to impact live birth rates, the exclusive influence of cryopreservation has not been elucidated. Here, we evaluate the effect of repeated cryopreservation on embryo health and implantation potential. Blastocyst-stage murine embryos were subjected to one, two or three freeze-thaw cycles with fresh embryos serving as a control. Outcomes assessed included post-thaw survival rate, allocation of cells to the inner cell mass (ICM) vs. trophectoderm cell lineages, implantation potential and offspring health. Post-thawing, embryos that were subjected to three freeze-thaw cycles had a significantly lower survival rates compared to embryos that had undergone one cycle (P<0.001). Additionally, the number of ICM cells was significantly reduced in embryos subjected to two or three freeze-thaw cycles compared to fresh or single-cycle embryos (P<0.001). No statistically significant differences were found for pregnancy rate, number of implantations, viable fetuses or resorption sites between treatment groups. We did however, find a non-significant yet interesting trend: three freeze-thaw cycles were associated with a 20% decrease in viable fetuses and a 20% increase in resorption sites compared to one freeze-thaw cycle group. These findings demonstrate that repeated cryopreservation adversely affects embryo health and may decrease implantation potential. Consequently, caution is advised regarding the repeated application of cryopreservation in IVF clinics, underscoring the need for further research to optimise cryopreservation protocols.

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Section: Discussionmentioning

confidence: 99%

Impact of repeated cryopreservation on embryo health and implantation potential

Li,

Chow

et al. 2023

Preprint

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“…For this, ovaries were collected at a local slaughterhouse and transported to the laboratory at 36°C in saline solution. At the laboratory, oocyte aspiration was performed, and the viable ones were selected for in vitro maturation, in vitro fertilization and in vitro culture of the embryos, applying a protocol previously described (Souza et al, 2018a). In vitro fertilization was performed with the use of semen from a Senepol steer.…”

Section: Estrous Synchronization Oocyte Collection and In Vitro Embmentioning

confidence: 99%

Managing embryonic and calves losses after twin pregnancies induced by transfer of in vitro-produced Nellore embryos

Souza¹,

Lubov

Paiva

et al. 2020

Zygote

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Summary Two farms applying reproductive technology for the Nellore beef cattle were selected. Both farms had the same technology programme of oestrous synchronization and embryo transfer, but management was different, especially regarding twins pregnancies. In the present study, we followed the farms from the moment of oestrous synchronization, embryo transfer (two per cow), until delivery and first care of the calves. In farm A, cows presenting twin pregnancies (5 from 13) were submitted to delivery induction, as well as calves and cows were monitored after birth. In farm B, such management was not followed with the twin pregnant cows (31 from 49). In both farms, freemartinism was detected, but this was not a problem as none of the animals would be selected for breeding. No dystocia was observed in farm A, while 48% of the twin pregnancies in farm B ended up in dystocia. Furthermore, the mortality rate of new-born calves in farm A was 10%, while in farm B it reached 32%. Although twin pregnancies remain a concern, we showed here that proper management during and after delivery minimizes animal and economic losses.

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