Cryoprotective Effect of Egg Yolk in Cryopreservation of Porcine Embryos

Fujino, Yukihiro; Ujisato, Y.; Endo, Katsuya; Tomizuka, Tsuneo; Kojima, Toshiyuki; Oguri, N.

doi:10.1006/cryo.1993.1028

Cited by 24 publications

(8 citation statements)

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“…urvival of porcine embryos after cryopreservation in liquid nitrogen has been confined to stage [1,2,[4][5][6] with limited embryonic development after transfer [7][8][9][10][11][12][13]. The absence of effective embryo cryopreservation technologies may not only result in a major loss of genetic diversity in this species, but also hinder application of reproductive technologies (e.g., embryo transfer, in vitro fertilization, embryo cloning) to the pig industry.…”

mentioning

confidence: 99%

Survival of Porcine Delipated Oocytes and Embryos after Cryopreservation by Freezing or Vitrification.

Nagashima

Cameron

Kuwayama

et al. 1999

Journal of Reproduction and Development

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Abstract. The present study examined whether delipated porcine oocytes and embryos at various stages of development can be cryopreserved by conventional slow cooling or vitrification. Most (93%) of the 27 delipated morulae developed to blastocysts after freezing with 1.5 M propanediol + 0.1 M sucrose. Late morulae and early blastocysts delipated at 2-4 cell stage and cultured in vitro survived freezing either with 1.5 M glycerol + 0.25 M sucrose (10/18, 56%) or 1.8 M ethylene glycol + 0.25 M sucrose (14/19, 74%). Delipated 2-4 cell stage embryos and oocytes could be cryopreserved by vitrification with 40% ethylene glycol, 1 M sucrose and 20% fetal calf serum. Half (7/14) of the vitrified, delipated embryos developed to blastocysts after thawing. Of 48 delipated oocytes, 27 (56%) maintained an intact outline of the ooplasm after vitrification and underwent subzonal sperm injection. Fertilization was confirmed in 12 (25%) of these oocytes and 3 (6%) developed to morula stage. This study also aimed at developing a non-invasive method for cryopreserving porcine embryos after reducing their cytoplasmic lipid content without micromanipulation. Morulae and early blastocysts were centrifuged in the presence of cytochalasin B and cryoprotectants and then frozen immediately. More than half (14/24, 58%) of the centrifuged morulae developed to blastocycts after freezing with 1.5 M propanediol + 0.1 M sucrose. Greater than 70% of centrifuged early blastocysts survived freezing either with 1.5 M propanediol (30/31, 97%), 1.5 M glycerol (22/29, 76%) or 1.8 M ethylene glycol (21/29, 72%). These results demonstrated that delipation (lipid removal) from porcine oocytes and embryos at various stages enables their cryopreservation. A new insight into the development of a non-invasive method for cryopreserving porcine embryos was also provided.

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confidence: 99%

Survival of Porcine Delipated Oocytes and Embryos after Cryopreservation by Freezing or Vitrification.

Nagashima

Cameron

Kuwayama

et al. 1999

Journal of Reproduction and Development

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“…The post thaw survival of porcine embryos has been investigated by several workers previously (reviewed by Nagashima et al, 1994) [8]. In these studies 1.4-1.5 M glycerol has been used exclusively as a cryoprotectant for freezing at the peri-hatching stage [4][5][6][7][12][13][14]. In the present study hatched blastocysts were also selected for freezing according to their size as the efficiency with which these can be frozen is known to decrease rapidly following hatching [4].…”

Section: Discussionmentioning

confidence: 99%

Improved Survival of Porcine Hatched Blastocysts Cryopreserved with Glycerol and Sucrose.

Nagashima¹,

Kashiwazaki²,

Rodney³

et al. 1995

Journal of Reproduction and Development

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“…Ce principe est couramment utilisé pour la congélation de la semence. Une vingtaine de porcelets est née dans le monde après transfert d'embryons ainsi congelés/ décongelés mais aucune des méthodes proposées n'a donné de résultats satisfaisants (Tableau 2) [12][13][14]. La présence de lipides a souvent été évoquée comme un obstacle à la congéla-tion de l'embryon porcin [15].…”

Section: Congélation Lenteunclassified

Cryopréservation et transfert des embryons porcins produits in vivo : état des lieux

Martinat-Botté

Berthelot

Plat

et al. 2006

Gynécologie Obstétrique & Fertilité

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Cryoprotective Effect of Egg Yolk in Cryopreservation of Porcine Embryos

Cited by 24 publications

References 0 publications

Survival of Porcine Delipated Oocytes and Embryos after Cryopreservation by Freezing or Vitrification.

Survival of Porcine Delipated Oocytes and Embryos after Cryopreservation by Freezing or Vitrification.

Improved Survival of Porcine Hatched Blastocysts Cryopreserved with Glycerol and Sucrose.

Cryopréservation et transfert des embryons porcins produits in vivo : état des lieux

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