Cryoprotective effect of collagen hydrolysates from squid skin on frozen shrimp and characterizations of its antifreeze peptides

Cao, Shuo; Cai, Jinxiu; Wang, Xiuzi; Zhou, Kenan; Liu, Liang; HE, Luyao; Xiang-yang, QI; Yang, Hua

doi:10.1016/j.lwt.2023.114443

Cited by 6 publications

(5 citation statements)

References 39 publications

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“…AFPTs are primarily derived from food proteins from different sources and can be efficiently prepared through specific enzyme digestion [ 15 ]. Initial new materials include meat/fish processing leftovers, such as animal/fish skin and others [ 17 , 18 , 19 ]. The critical proteases/enzymes responsible for the origin of Bacillus subtilis and Aspergillus oryzae , alcalase, flavourzyme, as well as papain, were subjected to enzymatic hydrolysis [ 20 ].…”

Section: Origin Of Antifreeze Proteins and Antifreeze Peptidesmentioning

confidence: 99%

“…They undergo hydrolysis or enzymatic hydrolysis to yield small-molecule AFPs or AFPTs. Generally, these AFPTs have molecular weights below 2000~3000 Da with specific amino acid residues, such as glycine and glutamic acid [ 17 ]. In a previous study, low molecular weight (<3000 kDa) fractions assumed a conspicuously preservative effect, with them having a higher THA value, fewer ice nuclei, and more prominent recrystallization inhibition activity [ 43 ].…”

Section: Freezing Protection Of Aquatic Products By Afps and Afptsmentioning

confidence: 99%

“…Based on the origin of these AFP and AFPT sources, their application on the aquatic products proved more compatible compared with others. To identify the antifreeze properties of these hydrolysates, the F-T cycle procedure was applied to evaluate the protein and lipid oxidation degree of muscle-rich products, such as fish/shrimp muscle or scallop adductor muscle [ 16 , 17 , 48 ]. Most protein oxidation indices, including sulfhydryl content, carbonyl content, salt-solubility, Ca 2+ -ATPase activity, and MP structure changes, have been found to demonstrate effective antifreeze properties by scavenging vitro free radicals and inhibiting ice crystal transformation [ 46 , 49 ].…”

Section: Freezing Protection Of Aquatic Products By Afps and Afptsmentioning

confidence: 99%

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Cryoprotective Effects and Quality Maintenance of Antifreeze Proteins and Peptides on Aquatic Products: A Review

Fan,

Geng,

et al. 2024

Foods

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Aquatic products are gaining popularity due to their delicacy and high nutrient value. However, they are perishable, with a short shelf-life. Frozen storage is associated with adverse effects, leading to protein oxidation and degradation, thereby altering the protein’s structural integrity and subsequently influencing the palatability of protein-based food products. To address these challenges, novel antifreeze peptides have gained significant attention. Antifreeze peptides are a class of small molecular weight proteins or protein hydrolysates that offer protection to organisms in frozen or sub-frozen environments. They offer distinct advantages over conventional commercial antifreeze agents and natural antifreeze proteins. This review provides an overview of the current state of research on antifreeze agents, elucidates their characteristics and mechanisms, and examines their applications in aquatic products. Furthermore, the article offers insights into the prospective development and application prospects of antifreeze peptides.

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Section: Origin Of Antifreeze Proteins and Antifreeze Peptidesmentioning

confidence: 99%

Section: Freezing Protection Of Aquatic Products By Afps and Afptsmentioning

confidence: 99%

Section: Freezing Protection Of Aquatic Products By Afps and Afptsmentioning

confidence: 99%

See 1 more Smart Citation

Cryoprotective Effects and Quality Maintenance of Antifreeze Proteins and Peptides on Aquatic Products: A Review

Fan,

Geng,

et al. 2024

Foods

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show abstract

“…Antifreeze peptides have a wide range of applications in the world of comestibles due to their economic viability and consistent ice-modulating ability. As evidenced by materials such as pigskin, 130 Takifugu obscurus skins, 18 squid skins, 131 and silver carp skin, researchers have recently become intensely interested in finding food antifreeze peptides. 132 Takifugu obscurus skins were enzymatically hydrolyzed by Yang et al (2023), 18 who meticulously collected the antifreeze peptide components using ice-affinity adsorption equipment.…”

Section: Absorption Of Ice Prisms�antifreezementioning

confidence: 99%

Coexisting with Ice Crystals: Cryogenic Preservation of Muscle Food─Mechanisms, Challenges, and Cutting-Edge Strategies

Xie,

Zhou,

Tan

et al. 2023

J. Agric. Food Chem.

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Cryopreservation, one of the most effective preservation methods, is essential for maintaining the safety and quality of food. However, there is no denying the fact that the quality of muscle food deteriorates as a result of the unavoidable production of ice. Advancements in cryoregulatory materials and techniques have effectively mitigated the adverse impacts of ice, thereby enhancing the standard of freezing preservation. The first part of this overview explains how ice forms, including the theoretical foundations of nucleation, growth, and recrystallization as well as the key influencing factors that affect each process. Subsequently, the impact of ice formation on the eating quality and nutritional value of muscle food is delineated. A systematic explanation of cutting-edge strategies based on nucleation intervention, growth control, and recrystallization inhibition is offered. These methods include antifreeze proteins, ice-nucleating proteins, antifreeze peptides, natural deep eutectic solvents, polysaccharides, amino acids, and their derivatives. Furthermore, advanced physical techniques such as electrostatic fields, magnetic fields, acoustic fields, liquid nitrogen, and supercooling preservation techniques are expounded upon, which effectively hinder the formation of ice crystals during cryopreservation. The paper outlines the difficulties and potential directions in ice inhibition for effective cryopreservation.

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“…As a result, it displays excellent growth attributes, including a short growth cycle and rapid reproduction rate, which enhances its potential for producing novel bioactive compounds. Collagens have been extracted from the skins of various squid species such as Doryteuthis singhalensis , Dosidicus gigas , and Loligo formosana [ 10 , 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

Stem-Cell-Regenerative and Protective Effects of Squid (Symplectoteuthis oualaniensis) Skin Collagen Peptides against H2O2-Induced Fibroblast Injury

Wei,

Jeevithan,

et al. 2024

Marine Drugs

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Recently, there has been a growing interest in collagen peptides derived from marine sources for their notable ability to protect skin cells against apoptosis induced by oxidants. Therefore, the current study aimed to investigate the fundamental properties of collagen peptides, including their physicochemical, thermal, structural, stem-cell-regenerative, and skin-cell-protective effects, in comparison to commercial collagen peptides. The acid-soluble (ASC) and pepsin-soluble (PSC) collagens exhibited three distinct bands on SDS-PAGE, namely α (α1 and α2), β, and γ chains, confirming a type I pattern. The thermal profiles obtained from TG and DSC analyses confirmed the denaturation of PSC and ASC at temperatures ranging from 51.94 to 56.4 °C and from 52.07 to 56.53 °C, respectively. The purified collagen peptides were analyzed using SDS-PAGE and MALDI-TOF mass spectrometry, revealing a mass range of 900–15,000 Da. Furthermore, the de novo peptide sequence analysis confirmed the presence of the Gly-X-Y repeating sequence in collagen peptides. Collagen peptide treatments significantly enhanced HFF-1 cell proliferation and migration compared to the control group. ELISA results confirmed the potential interactions between collagen peptides and HFF-1 cells through α2β1, α10β1, and α11β1 integrin receptors. Notably, collagen peptide treatment effectively restored the proliferation of HFF-1 cells damaged by H2O2. Consequently, the advantageous characteristics of squid skin collagen peptides highlight their promising role in regenerative medicine.

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Cryoprotective effect of collagen hydrolysates from squid skin on frozen shrimp and characterizations of its antifreeze peptides

Cited by 6 publications

References 39 publications

Cryoprotective Effects and Quality Maintenance of Antifreeze Proteins and Peptides on Aquatic Products: A Review

Cryoprotective Effects and Quality Maintenance of Antifreeze Proteins and Peptides on Aquatic Products: A Review

Coexisting with Ice Crystals: Cryogenic Preservation of Muscle Food─Mechanisms, Challenges, and Cutting-Edge Strategies

Stem-Cell-Regenerative and Protective Effects of Squid (Symplectoteuthis oualaniensis) Skin Collagen Peptides against H2O2-Induced Fibroblast Injury

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