2003
DOI: 10.1046/j.1365-2621.2003.00694.x
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Cryoprotection of protein by highly concentrated branched oligosaccharides

Abstract: Cryoprotection of protein by highly concentrated branched oligosaccharides (HBOS) was investigated by using a model solution containing bovine serum albumin (BSA). The glass transition temperature of the BSA solution with HBOS, determined by modulated differential scanning calorimetry, was )16.1°C, which is higher than that of sucrose at either 4 or 8% concentration by weight ()27.2°C). Also, changes in the unfrozen water fraction and their mobilities, determined by nuclear magnetic resonance, were used as ind… Show more

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Cited by 15 publications
(9 citation statements)
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References 44 publications
(43 reference statements)
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“…As presented in Table 4, the experimental and predicted values had no statistically significant difference (P<0.05) between them, and indicating that the freezing-point regulators (glycine, sodium chloride and D-sorbitol) interacted strongly with free water and participated in the water lattice through hydrogen bonds, and this presumably led to formation of extended region of hydrogen-bonded structured water in the vicinity of the cryoprotective molecule, which may stabilize surface hydration of protein and protect them from freeze-denaturation during storage (Auh et al 2003;Herrera and Mackie 2004;Kovačević et al 2007), and in the meantime the thawing phase transition range of Pacific white shrimp shifted to the lower temperature, and the content of unfreezable water increased while the initial freezing point linearly decreased, and corresponding peak value of latent heat and apparent specific heat also decreased.…”
Section: Parameters Optimization and Verificationmentioning
confidence: 94%
See 1 more Smart Citation
“…As presented in Table 4, the experimental and predicted values had no statistically significant difference (P<0.05) between them, and indicating that the freezing-point regulators (glycine, sodium chloride and D-sorbitol) interacted strongly with free water and participated in the water lattice through hydrogen bonds, and this presumably led to formation of extended region of hydrogen-bonded structured water in the vicinity of the cryoprotective molecule, which may stabilize surface hydration of protein and protect them from freeze-denaturation during storage (Auh et al 2003;Herrera and Mackie 2004;Kovačević et al 2007), and in the meantime the thawing phase transition range of Pacific white shrimp shifted to the lower temperature, and the content of unfreezable water increased while the initial freezing point linearly decreased, and corresponding peak value of latent heat and apparent specific heat also decreased.…”
Section: Parameters Optimization and Verificationmentioning
confidence: 94%
“…As clearly displayed in Table 3, the interaction effect between glycine and D-sorbitol content was very significant (P<0.01). These substances interact strongly with water and participate in the water lattice through hydrogen bonds, which lead to formation of extended region of hydrogen-bonded structured water in the vicinity of the cryoprotective molecule and increase of unfreezable water (Smolinska et al 1995;Auh et al 2003;Kovačević et al 2007). …”
Section: Unfreezable Water Mass Fraction (W U )mentioning
confidence: 99%
“…Surimi protein can be an excellent source for making noodles with high nutritional properties, well-balanced amino acid and acceptable flavor (Auh et al, 2003). Surimi protein is rich in lysine, which is usually deficient in cereal grains.…”
Section: Introductionmentioning
confidence: 99%
“…Surimi is a paste of fish meat frozen tasteless, odorless, highly viscous and elastic, but unstable to freezing in the absence of cryoprotectives such as sorbitol and glucose, mainly made of white muscle crushed, washed, drained and stabilized by the addition of cryoprotectives (Auh et al 2003). In addition carbohydrates, in particular sucrose was reported to inhibit the formation of biogenic amines in dry cured fish at a low storage temperature (4°C) (Zhang et al 2011).…”
Section: Introductionmentioning
confidence: 99%