Cryoprotectants and X-ray analysis on Passiflora seeds cryopreserved

Abstract

The study aimed to evaluate the internal morphology of Passiflora eichleriana Mast., Passiflora nitida Kunth. and Passiflora mucronata Lam. seeds cryopreserved with different cryoprotectants. The treatment were control (without cryoprotectant or cryopreservation), seeds cryopreserved without cryoprotectant, 1.73, 2.28, 2.60 or 2.71 M glycerol, 0.37, 0.46, 0.54 or 0.61 M sucrose and 0.37, 0.72, 1.04 or 1.35 M dimethylsulfoxide. The seeds were cryopreserved in liquid nitrogen (-196 °C) for seven days. The cryopreserved seeds were dipped in water bath (37 °C) for 5 minutes to defrost. The percentage of filled, empty, malformed and damaged seeds was determined. The seeds were analyzed by X-ray to verify damages caused by the cryopreservation or defrost. Our results demonstrate that X-ray is an efficient method to analyses cryopreserved seeds and the cryopreservation technique did not cause mechanical damage of P. eichleriana, P. nitida and P. mucronata seeds.

Advances in micropropagation, somatic embryogenesis, somatic hybridizations, genetic transformation and cryopreservation for Passiflora improvement

“…Furthermore, the occurrence of oxidative stress was analyzed at various phases of the protocol by measuring oxidative damage and the antioxidant defense enzymes Shoot tip V-Cryo-plate technique [ 102 ] P. pinnatistipula , P. tarminiana , P. mollissima To develop cryopreservation protocols for recalcitrant or intermediate seeds which provide a viable method of long-term germplasm conservation Seed Encapsulation-cryopreservation technique [ 104 ] P. alata , P. cincinnata , P. coriacea , P. edulis , P. edulis f. flavicarpa , P. foetida , P. giberti , P. micropetala , P. morifolia , P. nitida ) To test germination percentage of 10 species Seed Liquid nitrogen (− 196 °C) [ 107 ] P. pohlii To the development of in vitro preservation using axenic plant nodal segments Nodal segments Encapsulation-vitrification and vitrification techniques [ 108 ] P. edulis to achieve the efficient cryopreservation of plant embryos by providing their rapid (5 min), uniform permeation by Plant Vitrification Solution cryoprotectant Zygotic embryo Vacuum infiltration vitrification (VIV), [ 109 ] P. eichleriana Mast., P. nitida Kunth., P. mucronata Lam. To examine X-rays to check for any damage brought over by cryopreservation or defrosting Seed Liquid nitrogen (− 196 °C) [ 111 ] …”

“…Considering recent progress, prospects include improving the existing cryopreservation techniques and developing new ones. To examine damage causedd by cryopreservation or defrosting, X-rays are used to check for any damage (Table 3 ) [ 111 ].…”

X-RAY AND IMAGE ANALYSES OF SEEDS OF Passiflora cincinnata AND Passiflora morifolia

“…Automated methods allow the categorization of seeds through images, being efficient to permit the identification of seeds of various colors, the presence of mechanical damages, and the classification of different sizes. Literature has documented many studies about image analysis, in order to establish a pattern of vigor in seeds (KOBORI et al, 2012;FARIA et al, 2021).…”