Cryoprotectant transport through articular cartilage for long-term storage: experimental and modeling studies

Mukherjee, Indra N.; Li, Yang; Song, Yang; Long, Robert C.; Sambanis, Athanassios

doi:10.1016/j.joca.2008.03.027

Cited by 30 publications

(27 citation statements)

References 38 publications

(55 reference statements)

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“…Diffusion of Me 2 SO, formamide and 1,2-propanediol was the main limitation to mass transfer. The diffusivity measured by Mukherjee et al [30] for Me 2 SO, formamide, and 1,2-propanediol into cartilage tissue at room temperature was 4.63 × 10 −10 m 2 /s, 3.47 × 10 −10 m 2 /s, and 6.67 × 10 −10 m 2 /s, respectively. Based on Fick's second law of diffusion, the time it took for VS55 to permeate native cartilage and equilibrate with the surrounding bulk VS55 solution was approximately 2-3 hours.…”

Section: Discussionmentioning

confidence: 88%

Protocol Development for Vitrification of Tissue-Engineered Cartilage

Farooque

Chen²,

Schwartz³

et al. 2010

BioProcess J

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Section: Discussionmentioning

confidence: 88%

Protocol Development for Vitrification of Tissue-Engineered Cartilage

Farooque

Chen²,

Schwartz³

et al. 2010

BioProcess J

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“…Due to the importance of the CPA permeation characteristics to the cryopreservation of tissues, many methods have been developed to investigate the penetration rates of CPA in tissue samples, such as using nuclear magnetic resonance (NMR) techniques (Muldrew et al, 1996;Carsi et al, 2004;Mukherjee et al, 2008), high performance liquid chromatography (HPLC) (Carpenter and Dawson, 1991;Hu and Wolfinbarger, 1994;Pegg, 2006), radiotracer ( 35 S) (Elford, 1970), osmometer (Sharma et al, 2007;Jomha et al, 2009;Yu et al, 2010), conductivity meter (Chen et al, 2009), or electronic microbalance (Pegg, 2006). In this study, the quantity of Me 2 SO in ovine AC was measured using a spectrophotometer.…”

Section: Resultsmentioning

confidence: 99%

“…Investigation of Me 2 SO permeation into AC has been studied with ovine (Pegg et al, 2006b), porcine (Muldrew et al, 1996;Sharma et al, 2007;Jomha et al, 2009), bovine (Mukherjee et al, 2008), and human AC (Carsi et al, 2004). In these studies, the temperature levels were between approximately 0 and 37 °C.…”

Section: Resultsmentioning

confidence: 99%

“…Therefore, the surface concentration is equal to Kc s , where c s is the concentration of the bathing solution, and K is the partition coefficient of Me 2 SO between the tissue and the solution. In this research, K is set to 0.9 (Mukherjee et al, 2008). The partial differential equation was solved with MATLAB software.…”

Section: Diffusion Modelmentioning

confidence: 99%

“…The CPA permeation in AC at above zero temperatures has been reported in a few studies. Mukherjee et al (2008) studied the diffusions of high concentration single CPA [6.9 mol/L, dimethyl sulfoxide (Me 2 SO)] and multi-component CPA mixture (VS55, 3.1 mol/L Me 2 SO+2.2 mol/L 1,2-propanediol+3.1 mol/L formamide) in immature bovine AC. Sharma et al (2007) and Jomha et al (2009) examined the time-dependent permeations of Me 2 SO, propylene glycol (PG), ethylene glycol (EG), and glycerol into intact porcine AC at concentrations suitable for vitrification.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Permeation of dimethyl sulfoxide into articular cartilage at subzero temperatures

Zhang

Chen

2012

J. Zhejiang Univ. Sci. B

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Abstract:Osteochondral allografting has been proved to be a useful method to treat diseased or damaged areas of joint surfaces. Operational long-term stocks of grafts which supply a buffer between procurement and utilization would contribute to the commercialization or industrialization of this technology. Vitrification has been thought to be a promising method for successful preservation of articular cartilage (AC), but high concentration cryoprotectants (CPAs) are used which may cause high cellular toxicity. An effective way to reduce CPA toxicity is to increase CPA concentration gradually while the temperature is lowered. Understanding the mechanism of CPA permeation at subzero temperatures is important for designing the cryopreservation protocol. In this research, the permeation of dimethyl sulfoxide (Me 2 SO) in ovine AC at subzero temperatures was studied experimentally. Pretreated AC discs were exposed in Me 2 SO solutions for different time (0, 5, 15, 30, 50, 80, and 120 min) at three temperature levels (−10, −20, and −30 °C). The Me 2 SO concentration within the tissue was determined by ultraviolet (UV) spectrophotometry. The diffusion coefficients were estimated to be 0.85×10 −6 , 0.48×10 −6 , and 0.27×10 −6 cm 2 /s at −10, −20, and −30 °C, respectively, and the corresponding activation energy was 29.23 kJ/mol. Numerical simulation was performed to compare two Me 2 SO addition protocols, and the results demonstrated that the total loading duration could be effectively reduced with the knowledge of permeation kinetics.

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Imaging the distribution of iron oxide nanoparticles in hypothermic perfused tissues

Ring

Gao

Sharma

et al. 2019

Magnetic Resonance in Med

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PurposeHerein, we evaluate the use of MRI as a tool for assessing iron oxide nanoparticle (IONP) distribution within IONP perfused organs and vascularized composite allografts (VCAs) (i.e., hindlimbs) prepared for cryopreservation.MethodsMagnetic resonance imaging was performed on room‐temperature organs and VCAs perfused with IONPs and were assessed at 9.4 T. Quantitative T1 mapping and ‐weighted images were acquired using sweep imaging with Fourier transformation and gradient‐echo sequences, respectively. Verification of IONP localization was performed through histological assessment and microcomputer tomography.ResultsQuantitative imaging was achieved for organs and VCAs perfused with up to 642 mMFe (36 mgFe/mL), which is above previous demonstrations of upper limit detection in agarose (35.7mMFe [2 mgFe/mL]). The stability of IONPs in the perfusate had an effect on the quality of distribution and imaging within organs or VCA. Finally, MRI provided more accurate IONP localization than Prussian blue histological staining in this system, wherein IONPs remain primarily in the vasculature.ConclusionUsing MRI, we were able to assess the distribution of IONPs throughout organs and VCAs varying in complexity. Additional studies are necessary to better understand this system and validate the calibration between T1 measurements and IONP concentration.

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Cryoprotectant transport through articular cartilage for long-term storage: experimental and modeling studies

Abstract: This study provides a foundation for designing CPA addition and removal protocols for effective long-term storage of cartilage tissue using a novel approach to measure CPA permeation.

Cited by 30 publications

References 38 publications

Protocol Development for Vitrification of Tissue-Engineered Cartilage

Protocol Development for Vitrification of Tissue-Engineered Cartilage

Permeation of dimethyl sulfoxide into articular cartilage at subzero temperatures

Imaging the distribution of iron oxide nanoparticles in hypothermic perfused tissues

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